Restriction map for pF1KB8302
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstNI       BstXI       BstYI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvaI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TspRI       TthHB8I     XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwI           50  GATCC                GGATC(4/5)
AlwNI         187  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          133  CCTGAGG              CC^T[ACGT]AGG
BanI           20  GGTGCC               G^G[CT][GA]CC
BanII          27  GAGCCC               G[GA]GC[CT]^C
BbrPI         547  CACGTG               CAC^GTG
BmyI           27  GAGCCC               G[AGT]GC[ACT]^C
BsgI          518  CTGCAC               GTGCAG(16/14)
BsmAI         281  GAGAC                GTCTC(1/5)
Bsp1286I       27  GAGCCC               G[AGT]GC[ACT]^C
BsrFI         128  ACCGGC               [GA]^CCGG[CT]
BstEII        594  GGTGACC              G^GT[ACGT]ACC
BstPI         594  GGTGACC              G^GT[ACGT]ACC
Bsu36I        133  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        128  ACCGGC               [GA]^CCGG[CT]
DdeI          134  CTGAG                C^T[ACGT]AG
DpnII          50  GATC                 ^GATC
DraIII        117  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          307  CTCTTC               CTCTTC(1/4)
Eco81I        133  CCTGAGG              CC^T[ACGT]AGG
EcoNI         432  CCTTAACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       594  GGTGACC              G^GT[ACGT]ACC
EcoRI         607  GAATTC               G^AATTC
Ksp632I       307  CTCTTC               CTCTTC(1/4)
MboI           50  GATC                 ^GATC
NdeII          50  GATC                 ^GATC
PflMI         564  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         547  CACGTG               CAC^GTG
PmlI          547  CACGTG               CAC^GTG
PpuMI         188  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          151  CTGCAG               CTGCA^G
Sau3AI         50  GATC                 ^GATC
SmlI          275  CTCAAG               C^T[CT][GA]AG
Van91I        564  CCAGAAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           60  CCAGCTGGTGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   40 enzymes) #
###################################

AccII          47  CGCG                 CG^CG
              173  CGCG                
AflIII        509  ACACGT               A^C[GA][CT]GT
              546  ACACGT              
AspI           69  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              664  GACAATGTC           
AvaII         189  GGTCC                G^G[AT]CC
              647  GGTCC               
BcnI          198  CCGGG                CC^[GC]GG
              525  CCGGG               
BpmI          285  CTCCAG               CTGGAG(16/14)
              142  CTGGAG              
BsaAI         510  CACGTA               [CT]AC^GT[GA]
              547  CACGTG              
BseRI         238  GAGGAG               GAGGAG(10/8)
              529  GAGGAG              
BsmFI          81  GTCCC                GGGAC(10/14)
              218  GTCCC               
BspHI         476  TCATGA               T^CATGA
              641  TCATGA              
BspMI          17  GCAGGT               ACCTGC(4/8)
              186  GCAGGT              
BsrI           70  ACTGG                ACTGG(1/-1)
              264  CCAGT               
BssKI         198  CCGGG                ^CC[ACGT]GG
              525  CCGGG               
BstF5I        585  GGATG                GGATG(2/0)
              630  GGATG               
BstUI          47  CGCG                 CG^CG
              173  CGCG                
Cac8I          29  GCCCGC               GC[ACGT]^[ACGT]GC
               39  GCAGGC              
CfoI           46  GCGC                 GCG^C
              174  GCGC                
DraII         188  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              533  AGGCCCC             
EcoO109I      188  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              533  AGGCCCC             
EcoT14I       455  CCAAGG               C^C[AT][AT]GG
              538  CCAAGG              
FauI           14  CCCGC                CCCGC(4/6)
               30  CCCGC               
FokI          585  GGATG                GGATG(9/13)
              630  GGATG               
HhaI           46  GCGC                 GCG^C
              174  GCGC                
HinP1I         46  GCGC                 G^CGC
              174  GCGC                
MaeII         511  ACGT                 A^CGT
              548  ACGT                
MseI          407  TTAA                 T^TAA
              434  TTAA                
MvnI           47  CGCG                 CG^CG
              173  CGCG                
MwoI           39  GCAGGCAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              150  GCTGCAGGAGC         
NciI          198  CCGGG                CC^[GC]GG
              525  CCGGG               
PflFI          69  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              664  GACAATGTC           
PleI          283  GACTC                GAGTC(4/5)
              616  GAGTC               
PvuII          61  CAGCTG               CAG^CTG
              148  CAGCTG              
RcaI          476  TCATGA               T^CATGA
              641  TCATGA              
ScrFI         198  CCGGG                CC^[ACGT]GG
              525  CCGGG               
SfaNI           8  GATGC                GCATC(5/9)
              449  GCATC               
SfcI          151  CTGCAG               C^T[GA][CT]AG
              392  CTGTAG              
StyI          455  CCAAGG               C^C[AT][AT]GG
              538  CCAAGG              
TfiI          109  GAATC                G^A[AT]TC
              378  GATTC               
Tru9I         407  TTAA                 T^TAA
              434  TTAA                
Tth111I        69  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              664  GACAATGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsaJI       BsiYI       BslI        Cfr13I      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboII       MnlI        
MspA1I      MspI        NlaIII      NlaIV       RsaI        
Sau96I      TseI        Tsp45I      Tsp509I     
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