(Circular) (Six-base) MAPSORT of: pF1KB8293.seq Check: 3024 from: 1 to: 4019 pF1KB8293 4019 bp With 182 enzymes: SgfI * June 20, 2008 10:28 .. AccI GT'mk_AC Cuts at: 621 1057 621 Size: 436 3583 Acc65I G'GTAC_C Cuts at: 1035 1035 Size: 4019 AclI AA'CG_TT Cuts at: 780 3859 780 Size: 3079 940 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1844 2276 3023 3291 1844 Size: 432 747 268 2572 Fragments arranged by size: 2572 747 432 268 AflII C'TTAA_G Cuts at: 817 817 Size: 4019 AflIII A'CryG_T Cuts at: 1371 2475 3452 1371 Size: 1104 977 1938 Fragments arranged by size: 1938 1104 977 AgeI A'CCGG_T Cuts at: 2407 2407 Size: 4019 AlwNI CAG_nnn'CTG Cuts at: 862 1324 2891 862 Size: 462 1567 1990 Fragments arranged by size: 1990 1567 462 ApaLI G'TGCA_C Cuts at: 2789 2789 Size: 4019 ApoI r'AATT_y Cuts at: 377 927 1023 3372 3628 377 Size: 550 96 2349 256 768 Fragments arranged by size: 2349 768 550 256 96 AseI AT'TA_AT Cuts at: 20 20 Size: 4019 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4019 AvaI C'yCGr_G Cuts at: 1010 1039 1010 Size: 29 3990 BamHI G'GATC_C Cuts at: 190 1044 190 Size: 854 3165 BanI G'GyrC_C Cuts at: 1035 1449 1697 1732 1035 Size: 414 248 35 3322 Fragments arranged by size: 3322 414 248 35 BanII G_rGCy'C Cuts at: 277 1033 2063 277 Size: 756 1030 2233 Fragments arranged by size: 2233 1030 756 BbeI G_GCGC'C Cuts at: 1453 1701 1453 Size: 248 3771 BbsI GAAGACnn'nnnn_ Cuts at: 803 841 803 Size: 38 3981 BciVI GTATCCnnnnn_n' Cuts at: 1917 2678 4019 1917 Size: 761 1341 1917 Fragments arranged by size: 1917 1341 761 BclI T'GATC_A Cuts at: 1539 1539 Size: 4019 BglII A'GATC_T Cuts at: 363 726 1534 3571 363 Size: 363 808 2037 811 Fragments arranged by size: 2037 811 808 363 BlpI GC'TnA_GC Cuts at: 1131 1131 Size: 4019 Bme1580I G_kGCm'C Cuts at: 1644 1737 2793 1644 Size: 93 1056 2870 Fragments arranged by size: 2870 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1231 1312 1647 3791 1231 Size: 81 335 2144 1459 Fragments arranged by size: 2144 1459 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 4019 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 901 901 Size: 4019 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 198 1195 2074 2566 2864 198 Size: 997 879 492 298 1353 Fragments arranged by size: 1353 997 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3723 3723 Size: 4019 BsaAI yAC'GTr Cuts at: 1372 1372 Size: 4019 BsaBI GATnn'nnATC Cuts at: 6 1533 3570 6 Size: 1527 2037 455 Fragments arranged by size: 2037 1527 455 BsaHI Gr'CG_yC Cuts at: 1450 1698 2400 3896 1450 Size: 248 702 1496 1573 Fragments arranged by size: 1573 1496 702 248 BsaWI w'CCGG_w Cuts at: 1208 1729 2407 2681 2828 3239 1208 Size: 521 678 274 147 411 1988 Fragments arranged by size: 1988 678 521 411 274 147 BseYI C'CCAG_C Cuts at: 1444 2202 2779 3275 1444 Size: 758 577 496 2188 Fragments arranged by size: 2188 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 425 529 425 Size: 104 3915 BsiEI CG_ry'CG Cuts at: 104 1561 1607 2815 104 Size: 1457 46 1208 1308 Fragments arranged by size: 1457 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 227 1033 1811 2001 2793 227 Size: 806 778 190 792 1453 Fragments arranged by size: 1453 806 792 778 190 BsmI GAATG_Cn' Cuts at: 767 987 767 Size: 220 3799 Bsp1286I G_dGCh'C Cuts at: 227 277 1033 1644 1737 1811 2001 2063 2793 Size: 50 756 611 93 74 190 62 730 Cuts at: 2793 227 Size: 1453 Fragments arranged by size: 1453 756 730 611 190 93 74 62 50 BspEI T'CCGG_A Cuts at: 3239 3239 Size: 4019 BspHI T'CATG_A Cuts at: 3195 3195 Size: 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1069 1283 1585 1966 1069 Size: 214 302 381 3122 Fragments arranged by size: 3122 381 302 214 BsrBI CCG'CTC Cuts at: 50 2311 2365 3849 4014 50 Size: 2261 54 1484 165 55 Fragments arranged by size: 2261 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 344 1931 344 Size: 1587 2432 BsrFI r'CCGG_y Cuts at: 2017 2198 2407 2017 Size: 181 209 3629 Fragments arranged by size: 3629 209 181 BssSI C'ACGA_G Cuts at: 2290 2648 2290 Size: 358 3661 BstAPI GCAn_nnn'nTGC Cuts at: 1396 1396 Size: 4019 BstBI TT'CG_AA Cuts at: 569 612 2380 569 Size: 43 1768 2208 Fragments arranged by size: 2208 1768 43 BstXI CCAn_nnnn'nTGG Cuts at: 879 879 Size: 4019 BstYI r'GATC_y Cuts at: 1 190 363 726 1044 1511 1534 1869 2115 Size: 189 173 363 318 467 23 335 246 Cuts at: 2115 3127 3213 3225 3571 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 467 449 363 346 335 318 246 189 173 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4019 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1969 2150 2313 1969 Size: 181 163 3675 Fragments arranged by size: 3675 181 163 BtsI GCAGTG_nn' Cuts at: 136 1262 136 Size: 1126 2893 DraI TTT'AAA Cuts at: 1018 1018 Size: 4019 DraIII CAC_nnn'GTG Cuts at: 448 448 Size: 4019 DrdI GACnn_nn'nnGTC Cuts at: 1725 2583 1725 Size: 858 3161 EaeI y'GGCC_r Cuts at: 1604 1778 2169 2196 3541 1604 Size: 174 391 27 1345 2082 Fragments arranged by size: 2082 1345 391 174 27 EagI C'GGCC_G Cuts at: 1604 1604 Size: 4019 EarI CTCTTCn'nnn_ Cuts at: 398 2042 2252 398 Size: 1644 210 2165 Fragments arranged by size: 2165 1644 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 200 2227 2537 2683 3829 200 Size: 2027 310 146 1146 390 Fragments arranged by size: 2027 1146 390 310 146 EcoICRI GAG'CTC Cuts at: 1031 1031 Size: 4019 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 901 1844 2276 3023 3291 901 Size: 943 432 747 268 1629 Fragments arranged by size: 1629 943 747 432 268 EcoO109I rG'GnC_Cy Cuts at: 1158 1158 Size: 4019 EcoRI G'AATT_C Cuts at: 1023 1023 Size: 4019 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2038 2038 Size: 4019 FspI TGC'GCA Cuts at: 1800 1800 Size: 4019 HaeII r_GCGC'y Cuts at: 1453 1701 2723 3702 1453 Size: 248 1022 979 1770 Fragments arranged by size: 1770 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 556 588 703 735 1861 1891 1893 1923 556 Size: 32 115 32 1126 30 2 30 2652 Fragments arranged by size: 2652 1126 115 32 32 30 30 2 HincII GTy'rAC Cuts at: 318 1058 318 Size: 740 3279 HindIII A'AGCT_T Cuts at: 428 428 Size: 4019 KasI G'GCGC_C Cuts at: 1449 1697 1449 Size: 248 3771 KpnI G_GTAC'C Cuts at: 1039 1039 Size: 4019 MluI A'CGCG_T Cuts at: 3452 3452 Size: 4019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 551 2690 2874 551 Size: 2139 184 1696 Fragments arranged by size: 2139 1696 184 MscI TGG'CCA Cuts at: 1780 1780 Size: 4019 MslI CAynn'nnrTG Cuts at: 2008 2135 2008 Size: 127 3892 MspA1I CmG'CkG Cuts at: 1130 1444 1804 2817 3062 1130 Size: 314 360 1013 245 2087 Fragments arranged by size: 2087 1013 360 314 245 NaeI GCC'GGC Cuts at: 2200 2200 Size: 4019 NarI GG'CG_CC Cuts at: 1450 1698 1450 Size: 248 3771 NcoI C'CATG_G Cuts at: 107 107 Size: 4019 NgoMIV G'CCGG_C Cuts at: 2198 2198 Size: 4019 NspI r_CATG'y Cuts at: 212 1072 1572 2479 212 Size: 860 500 907 1752 Fragments arranged by size: 1752 907 860 500 PciI A'CATG_T Cuts at: 2475 2475 Size: 4019 PmeI GTTT'AAAC Cuts at: 1018 1018 Size: 4019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 765 797 3158 3190 765 Size: 32 2361 32 1594 Fragments arranged by size: 2361 1594 32 32 PspXI vC'TCGA_Gb Cuts at: 1010 1010 Size: 4019 PstI C_TGCA'G Cuts at: 170 1066 1751 170 Size: 896 685 2438 Fragments arranged by size: 2438 896 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4019 PvuII CAG'CTG Cuts at: 1444 1804 1444 Size: 360 3659 RsrII CG'GwC_CG Cuts at: 2214 2214 Size: 4019 SacI G_AGCT'C Cuts at: 1033 1033 Size: 4019 SalI G'TCGA_C Cuts at: 1056 1056 Size: 4019 SapI GCTCTTCn'nnn_ Cuts at: 2042 2252 2042 Size: 210 3809 SbfI CC_TGCA'GG Cuts at: 1066 1066 Size: 4019 ScaI AGT'ACT Cuts at: 3536 3536 Size: 4019 SfcI C'TryA_G Cuts at: 33 166 638 1062 1747 2740 2931 33 Size: 133 472 424 685 993 191 1121 Fragments arranged by size: 1121 993 685 472 424 191 133 SfoI GGC'GCC Cuts at: 1451 1699 1451 Size: 248 3771 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4019 SmaI CCC'GGG Cuts at: 1041 1041 Size: 4019 SmlI C'TyrA_G Cuts at: 213 817 1010 1174 2089 2581 2843 213 Size: 604 193 164 915 492 262 1389 Fragments arranged by size: 1389 915 604 492 262 193 164 SpeI A'CTAG_T Cuts at: 67 697 67 Size: 630 3389 SphI G_CATG'C Cuts at: 1072 1572 1072 Size: 500 3519 StuI AGG'CCT Cuts at: 294 294 Size: 4019 StyI C'CwwG_G Cuts at: 107 1153 1507 107 Size: 1046 354 2619 Fragments arranged by size: 2619 1046 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1602 1735 2194 2403 1602 Size: 133 459 209 3218 Fragments arranged by size: 3218 459 209 133 TatI w'GTAC_w Cuts at: 3534 3534 Size: 4019 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3536 3568 3536 Size: 32 3987 Tth111I GACn'n_nGTC Cuts at: 1816 1816 Size: 4019 XbaI T'CTAG_A Cuts at: 729 1050 729 Size: 321 3698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1514 1514 Size: 4019 XhoI C'TCGA_G Cuts at: 1010 1010 Size: 4019 XmaI C'CCGG_G Cuts at: 1039 1039 Size: 4019 XmnI GAAnn'nnTTC Cuts at: 608 800 608 Size: 192 3827 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI ZraI