Restriction map for pF1KB8284
 (Linear) (Six-base) MAPSORT of: KIBB8284.seq  Check: 6756  from: 1  to: 1236



KIBB8284.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AccI GT'mk_AC

Cuts at:      0    1172    1236 
   Size:       1172      64
 
Acc65I G'GTAC_C

Cuts at:      0     556    1236 
   Size:        556     680
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     445     517     974    1219    1236 
   Size:        244     201      72     457     245      17

  Fragments arranged by size: 

                 457     245     244     201      72      17
 
AflIII A'CryG_T

Cuts at:      0      95     442    1236 
   Size:         95     347     794

  Fragments arranged by size: 

                 794     347      95
 
AhdI GACnn_n'nnGTC

Cuts at:      0     498    1236 
   Size:        498     738
 
AleI CACnn'nnGTG

Cuts at:      0     689    1039    1236 
   Size:        689     350     197

  Fragments arranged by size: 

                 689     350     197
 
AlwNI CAG_nnn'CTG

Cuts at:      0     468     997    1236 
   Size:        468     529     239

  Fragments arranged by size: 

                 529     468     239
 
ApaI G_GGCC'C

Cuts at:      0     940    1236 
   Size:        940     296
 
AvaI C'yCGr_G

Cuts at:      0     449     488     687    1236 
   Size:        449      39     199     549

  Fragments arranged by size: 

                 549     449     199      39
 
BamHI G'GATC_C

Cuts at:      0       8    1236 
   Size:          8    1228
 
BanI G'GyrC_C

Cuts at:      0     556    1236 
   Size:        556     680
 
BanII G_rGCy'C

Cuts at:      0     940    1236 
   Size:        940     296
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     494    1236 
   Size:        494     742
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1040    1236 
   Size:       1040     196
 
BclI T'GATC_A

Cuts at:      0     333     917    1236 
   Size:        333     584     319

  Fragments arranged by size: 

                 584     333     319
 
BglI GCCn_nnn'nGGC

Cuts at:      0     171    1236 
   Size:        171    1065
 
BglII A'GATC_T

Cuts at:      0    1109    1236 
   Size:       1109     127
 
Bme1580I G_kGCm'C

Cuts at:      0     450     940    1236 
   Size:        450     490     296

  Fragments arranged by size: 

                 490     450     296
 
BmgBI CAC'GTC

Cuts at:      0     193    1236 
   Size:        193    1043
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     546    1036    1236 
   Size:         79     467     490     200

  Fragments arranged by size: 

                 490     467     200      79
 
Bpu10I CC'TnA_GC

Cuts at:      0     429    1236 
   Size:        429     807
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      62     145     540    1236 
   Size:         62      83     395     696

  Fragments arranged by size: 

                 696     395      83      62
 
BsaWI w'CCGG_w

Cuts at:      0     197     869    1236 
   Size:        197     672     367

  Fragments arranged by size: 

                 672     367     197
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     752    1236 
   Size:        752     484
 
BseYI C'CCAG_C

Cuts at:      0      13     218     579     613    1236 
   Size:         13     205     361      34     623

  Fragments arranged by size: 

                 623     361     205      34      13
 
BsiHKAI G_wGCw'C

Cuts at:      0      65    1236 
   Size:         65    1171
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     183    1236 
   Size:        183    1053
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     450     940    1236 
   Size:         65     385     490     296

  Fragments arranged by size: 

                 490     385     296      65
 
BspHI T'CATG_A

Cuts at:      0    1094    1236 
   Size:       1094     142
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     791    1013    1236 
   Size:        791     222     223

  Fragments arranged by size: 

                 791     223     222
 
BsrBI CCG'CTC

Cuts at:      0       6     704    1236 
   Size:          6     698     532

  Fragments arranged by size: 

                 698     532       6
 
BsrDI GCAATG_nn'

Cuts at:      0     353    1236 
   Size:        353     883
 
BsrFI r'CCGG_y

Cuts at:      0     170    1236 
   Size:        170    1066
 
BsrGI T'GTAC_A

Cuts at:      0     107    1236 
   Size:        107    1129
 
BssHII G'CGCG_C

Cuts at:      0      17    1236 
   Size:         17    1219
 
BssSI C'ACGA_G

Cuts at:      0     478    1236 
   Size:        478     758
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     594    1236 
   Size:        594     642
 
BstYI r'GATC_y

Cuts at:      0       8     228    1109    1236 
   Size:          8     220     881     127

  Fragments arranged by size: 

                 881     220     127       8
 
BtgI C'CryG_G

Cuts at:      0     792    1236 
   Size:        792     444
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     198    1236 
   Size:        198    1038
 
BtsI GCAGTG_nn'

Cuts at:      0     287     771     915    1236 
   Size:        287     484     144     321

  Fragments arranged by size: 

                 484     321     287     144
 
DraI TTT'AAA

Cuts at:      0    1217    1236 
   Size:       1217      19
 
DraIII CAC_nnn'GTG

Cuts at:      0     531    1236 
   Size:        531     705
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1180    1236 
   Size:       1180      56
 
EaeI y'GGCC_r

Cuts at:      0     470     623    1236 
   Size:        470     153     613

  Fragments arranged by size: 

                 613     470     153
 
EarI CTCTTCn'nnn_

Cuts at:      0    1182    1236 
   Size:       1182      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     244     445     517     546     974    1036    1219 
   Size:         79     165     201      72      29     428      62     183

Cuts at:   1219    1236 
   Size:         17

  Fragments arranged by size: 

                 428     201     183     165      79      72      62      29
                  17
 
EcoO109I rG'GnC_Cy

Cuts at:      0     492     640    1236 
   Size:        492     148     596

  Fragments arranged by size: 

                 596     492     148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     774    1236 
   Size:        774     462
 
FspI TGC'GCA

Cuts at:      0     763    1236 
   Size:        763     473
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     484     516     666     698     806     838    1096    1101 
   Size:        484      32     150      32     108      32     258       5

Cuts at:   1101    1128    1133    1236 
   Size:         27       5     103

  Fragments arranged by size: 

                 484     258     150     108     103      32      32      32
                  27       5       5
 
HincII GTy'rAC

Cuts at:      0    1173    1236 
   Size:       1173      63
 
KpnI G_GTAC'C

Cuts at:      0     560    1236 
   Size:        560     676
 
MscI TGG'CCA

Cuts at:      0     472    1236 
   Size:        472     764
 
MslI CAynn'nnrTG

Cuts at:      0     689    1039    1236 
   Size:        689     350     197

  Fragments arranged by size: 

                 689     350     197
 
MspA1I CmG'CkG

Cuts at:      0     222     672    1236 
   Size:        222     450     564

  Fragments arranged by size: 

                 564     450     222
 
NspI r_CATG'y

Cuts at:      0      99     310     446    1236 
   Size:         99     211     136     790

  Fragments arranged by size: 

                 790     211     136      99
 
PciI A'CATG_T

Cuts at:      0      95     442    1236 
   Size:         95     347     794

  Fragments arranged by size: 

                 794     347      95
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     531    1236 
   Size:        531     705
 
PfoI T'CCnGG_A

Cuts at:      0     562    1236 
   Size:        562     674
 
PpuMI rG'GwC_Cy

Cuts at:      0     492     640    1236 
   Size:        492     148     596

  Fragments arranged by size: 

                 596     492     148
 
PspOMI G'GGCC_C

Cuts at:      0     936    1236 
   Size:        936     300
 
PspXI vC'TCGA_Gb

Cuts at:      0     687    1236 
   Size:        687     549
 
PstI C_TGCA'G

Cuts at:      0     365     551    1236 
   Size:        365     186     685

  Fragments arranged by size: 

                 685     365     186
 
PvuII CAG'CTG

Cuts at:      0     222    1236 
   Size:        222    1014
 
SalI G'TCGA_C

Cuts at:      0    1171    1236 
   Size:       1171      65
 
SfcI C'TryA_G

Cuts at:      0     361     547    1236 
   Size:        361     186     689

  Fragments arranged by size: 

                 689     361     186
 
SmaI CCC'GGG

Cuts at:      0     451    1236 
   Size:        451     785
 
SmlI C'TyrA_G

Cuts at:      0     687    1236 
   Size:        687     549
 
StuI AGG'CCT

Cuts at:      0    1061    1236 
   Size:       1061     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     532     959    1236 
   Size:        532     427     277

  Fragments arranged by size: 

                 532     427     277
 
TatI w'GTAC_w

Cuts at:      0     107    1236 
   Size:        107    1129
 
XhoI C'TCGA_G

Cuts at:      0     687    1236 
   Size:        687     549
 
XmaI C'CCGG_G

Cuts at:      0     449    1236 
   Size:        449     787
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbsI    BciVI     BclI     BglI
    BglII Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BsaWI    BseRI
    BseYI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EarI  Eco57MI EcoO109I
     FalI     FspI    Hin4I   HincII     KpnI     MscI     MslI   MspA1I
     NspI     PciI    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI
    PvuII     SalI     SfcI     SmaI     SmlI     StuI    TaqII     TatI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BfrBI     BlpI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI
    BspEI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PpiI    PshAI     PsiI     PsrI
     PvuI    RsrII     SacI    SacII    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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