Restriction map for pF1KB8275
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SbfI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     Van91I      XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          370  GTAGAC               GT^[AC][GT]AC
AccII         153  CGCG                 CG^CG
AcyI          205  GACGCC               G[GA]^CG[CT]C
AfaI          600  GTAC                 GT^AC
AvaII         137  GGACC                G^G[AT]CC
BanI          163  GGCACC               G^G[CT][GA]CC
BciVI         877  GTATCC               GTATCC(6/5)
BfaI          812  CTAG                 C^TAG
BglI           85  GCCATGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         333  AGATCT               A^GATCT
BsaHI         205  GACGCC               G[GA]^CG[CT]C
BsgI          375  CTGCAC               GTGCAG(16/14)
BsrFI         537  GCCGGT               [GA]^CCGG[CT]
BssSI         836  CTCGTG               CACGAG(-5/-1)
BstEII        850  GGTGACC              G^GT[ACGT]ACC
BstF5I        225  GGATG                GGATG(2/0)
BstNI         881  CCAGG                CC^[AT]GG
BstPI         850  GGTGACC              G^GT[ACGT]ACC
BstUI         153  CGCG                 CG^CG
CfoI          179  GCGC                 GCG^C
Cfr10I        537  GCCGGT               [GA]^CCGG[CT]
DraI          433  TTTAAA               TTT^AAA
EcoNI         394  CCTATCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       850  GGTGACC              G^GT[ACGT]ACC
EcoRII        881  CCAGG                ^CC[AT]GG
FokI          225  GGATG                GGATG(9/13)
HaeII         178  AGCGCC               [GA]GCGC^[CT]
HhaI          179  GCGC                 GCG^C
Hin1I         205  GACGCC               G[GA]^CG[CT]C
HinP1I        179  GCGC                 G^CGC
MaeI          812  CTAG                 C^TAG
MaeIII        851  GTGAC                ^GT[ACGT]AC
MvaI          881  CCAGG                CC^[AT]GG
MvnI          153  CGCG                 CG^CG
PleI          184  GAGTC                GAGTC(4/5)
RsaI          600  GTAC                 GT^AC
SapI          830  GAAGAGC              GCTCTTC(1/4)
ScaI          599  AGTACT               AGT^ACT
StuI          306  AGGCCT               AGG^CCT
TaqI          775  TCGA                 T^CGA
Tsp45I        851  GTGAC                ^GT[GC]AC
TthHB8I       775  TCGA                 T^CGA
XbaI          811  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          355  AAATTT               [GA]^AATT[CT]
              548  AAATTT              
ApoI          355  AAATTT               [GA]^AATT[CT]
              548  AAATTT              
AspHI         634  GAGCTC               G[AT]GC[AT]^C
              833  GAGCTC              
BanII         634  GAGCTC               G[GA]GC[CT]^C
              833  GAGCTC              
BcnI          134  CCGGG                CC^[GC]GG
              140  CCGGG               
BmyI          634  GAGCTC               G[AGT]GC[ACT]^C
              833  GAGCTC              
BpmI          569  CTCCAG               CTGGAG(16/14)
              158  CTGGAG              
BsiHKAI       634  GAGCTC               G[AT]GC[AT]^C
              833  GAGCTC              
Bsp1286I      634  GAGCTC               G[AGT]GC[ACT]^C
              833  GAGCTC              
BsrI          209  CCAGT                ACTGG(1/-1)
              703  CCAGT               
BstXI         732  CCACAATGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              855  CCAGCAGAGTGG        
Cac8I          67  GCGGGC               GC[ACGT]^[ACGT]GC
               71  GCGAGC              
Cfr13I        137  GGACC                G^G[ACGT]CC
              512  GGGCC               
DdeI          310  CTTAG                C^T[ACGT]AG
              745  CTTAG               
DsaI           86  CCATGG               C^C[GA][CT]GG
              641  CCATGG              
EcoT14I        86  CCATGG               C^C[AT][AT]GG
              641  CCATGG              
HgaI          205  GACGC                GACGC(5/10)
              154  GCGTC               
NciI          134  CCGGG                CC^[GC]GG
              140  CCGGG               
NcoI           86  CCATGG               C^CATGG
              641  CCATGG              
SacI          634  GAGCTC               GAGCT^C
              833  GAGCTC              
Sau96I        137  GGACC                G^G[ACGT]CC
              512  GGGCC               
StyI           86  CCATGG               C^C[AT][AT]GG
              641  CCATGG              
TfiI          439  GAATC                G^A[AT]TC
              625  GAATC               
TspRI         704  CAGTG                CA[GC]TG[ACGT][ACGT]^
              802  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       BssKI       
BstYI       DpnII       EarI        FauI        Fnu4HI      
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      ScrFI       
Tru9I       TseI        Tsp509I     XhoII       
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