Restriction map for pF1KB8271
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
AlwI        AocI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AvrII       BalI        BamHI       BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstXI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        Eam1105I    EarI        
Eco81I      EcoRI       EcoRV       EcoT22I     FbaI        
HincII      HindII      HindIII     HinfI       HpaI        
KpnI        Ksp632I     MaeI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        MunI        NcoI        
NdeI        NdeII       NheI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SalI        SapI        
Sau3AI      ScaI        SexAI       SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspBI       
SspI        SwaI        TfiI        Tru9I       Tsp509I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AcyI          559  GGCGCC               G[GA]^CG[CT]C
AflIII        383  ACGTGT               A^C[GA][CT]GT
Aor51HI       125  AGCGCT               AGC^GCT
AspHI         438  GAGCAC               G[AT]GC[AT]^C
AspI          177  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           88  CCCGAG               C^[CT]CG[GA]G
AvaII         725  GGACC                G^G[AT]CC
AviII         476  TGCGCA               TGC^GCA
BbeI          559  GGCGCC               GGCGC^C
BbsI          862  GAAGAC               GAAGAC(2/6)
BbvCI         573  CCTCAGC              CCTCAGC(-5/-2)
BglI          820  GCCTTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          207  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      207  GCTCAGC              GC^T[ACGT]AGC
BpuAI         862  GAAGAC               GAAGAC(2/6)
BsaHI         559  GGCGCC               G[GA]^CG[CT]C
BsaI          250  GAGACC               GGTCTC(1/5)
BsiHKAI       438  GAGCAC               G[AT]GC[AT]^C
BsmBI         316  GAGACG               CGTCTC(1/5)
BsoBI          88  CCCGAG               C^[CT]CG[GA]G
BsrFI         649  GCCGGC               [GA]^CCGG[CT]
BstAPI        420  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        219  GGTCACC              G^GT[ACGT]ACC
BstPI         219  GGTCACC              G^GT[ACGT]ACC
CelII         207  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        649  GCCGGC               [GA]^CCGG[CT]
EagI          390  CGGCCG               C^GGCCG
EclXI         390  CGGCCG               C^GGCCG
Eco47III      125  AGCGCT               AGC^GCT
Eco52I        390  CGGCCG               C^GGCCG
EcoNI         738  CCTGGACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       219  GGTCACC              G^GT[ACGT]ACC
FseI          648  GGCCGGCC             GGCCGG^CC
FspI          476  TGCGCA               TGC^GCA
HgaI          318  GACGC                GACGC(5/10)
Hin1I         559  GGCGCC               G[GA]^CG[CT]C
KasI          559  GGCGCC               G^GCGCC
KspI          408  CCGCGG               CCGC^GG
MaeII         383  ACGT                 A^CGT
MboII         862  GAAGA                GAAGA(8/7)
MspA1I        408  CCGCGG               C[AC]G^C[GT]G
NaeI          649  GCCGGC               GCC^GGC
NarI          559  GGCGCC               GG^CGCC
NgoMIV        649  GCCGGC               G^CCGGC
NotI          389  GCGGCCGC             GC^GGCCGC
NspI          866  ACATGC               [GA]CATG^[CT]
PflFI         177  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacII         408  CCGCGG               CCGC^GG
SbfI          807  CCTGCAGG             CCTGCA^GG
SfiI          819  GGCCTTCCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          559  GGCGCC               GGC^GCC
SmlI          835  CTCAAG               C^T[CT][GA]AG
Sse8387I      807  CCTGCAGG             CCTGCA^GG
TaqI          848  TCGA                 T^CGA
Tth111I       177  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       848  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI          405  GTAC                 GT^AC
              537  GTAC                
AlwNI         175  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
              226  CAGGAACTG           
BanI          559  GGCGCC               G^G[CT][GA]CC
              916  GGCACC              
BpmI          286  CTGGAG               CTGGAG(16/14)
              781  CTGGAG              
BsiEI         390  CGGCCG               CG[GA][CT]^CG
              732  CGACCG              
DsaI          408  CCGCGG               C^C[GA][CT]GG
              911  CCGTGG              
EcoT14I        53  CCAAGG               C^C[AT][AT]GG
              335  CCAAGG              
HphI          747  GGTGA                GGTGA(8/7)
              221  TCACC               
MaeIII         28  GTCAC                ^GT[ACGT]AC
              220  GTCAC               
RsaI          405  GTAC                 GT^AC
              537  GTAC                
SfaNI          44  GATGC                GCATC(5/9)
              505  GATGC               
StyI           53  CCAAGG               C^C[AT][AT]GG
              335  CCAAGG              
Tsp45I         28  GTCAC                ^GT[GC]AC
              220  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        BanII       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrBI       BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DraII       EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MnlI        MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PstI        
Sau96I      ScrFI       SfcI        StuI        TseI        
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com