Restriction map for pF1KB8255
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaII       AviII       AvrII       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MroI        MseI        
MspA1I      MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     XbaI        XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AcsI          158  GAATTC               [GA]^AATT[CT]
AfaI          827  GTAC                 GT^AC
Alw44I        855  GTGCAC               G^TGCAC
AlwNI         886  CAGACTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         855  GTGCAC               G^TGCAC
ApoI          158  GAATTC               [GA]^AATT[CT]
BamHI         610  GGATCC               G^GATCC
BbsI          552  GAAGAC               GAAGAC(2/6)
BcnI           81  CCCGG                CC^[GC]GG
BfaI          688  CTAG                 C^TAG
BglII         770  AGATCT               A^GATCT
BpuAI         552  GAAGAC               GAAGAC(2/6)
BsaI          145  GAGACC               GGTCTC(1/5)
BsmBI         495  CGTCTC               CGTCTC(1/5)
BspMI         930  GCAGGT               ACCTGC(4/8)
BsrFI         350  ACCGGC               [GA]^CCGG[CT]
BssSI         291  CACGAG               CACGAG(-5/-1)
CfoI          261  GCGC                 GCG^C
Cfr10I        350  ACCGGC               [GA]^CCGG[CT]
DraII         756  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DraIII        164  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          511  GACAAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      756  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         158  GAATTC               G^AATTC
EcoT14I       314  CCAAGG               C^C[AT][AT]GG
HhaI          261  GCGC                 GCG^C
HinP1I        261  GCGC                 G^CGC
HinfI         888  GACTC                G^A[ACGT]TC
MaeI          688  CTAG                 C^TAG
MboII         552  GAAGA                GAAGA(8/7)
NciI           81  CCCGG                CC^[GC]GG
PflMI         647  CCATGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          888  GACTC                GAGTC(4/5)
RsaI          827  GTAC                 GT^AC
SexAI         914  ACCTGGT              A^CC[AT]GGT
SfaNI         764  GCATC                GCATC(5/9)
StyI          314  CCAAGG               C^C[AT][AT]GG
Tsp509I       159  AATT                 ^AATT
Van91I        647  CCATGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AspHI         170  GTGCTC               G[AT]GC[AT]^C
              855  GTGCAC              
AvaI          104  CTCGAG               C^[CT]CG[GA]G
              371  CTCGAG              
BanI           25  GGCACC               G^G[CT][GA]CC
              283  GGCACC              
BglI           38  GCCACAACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              569  GCCACACAGGC         
BpmI          602  CTCCAG               CTGGAG(16/14)
              626  CTGGAG              
BseRI         213  CTCCTC               GAGGAG(10/8)
              339  CTCCTC              
BsiHKAI       170  GTGCTC               G[AT]GC[AT]^C
              855  GTGCAC              
BsmFI         509  GGGAC                GGGAC(10/14)
              698  GGGAC               
BsoBI         104  CTCGAG               C^[CT]CG[GA]G
              371  CTCGAG              
BstEII         51  GGTAACC              G^GT[ACGT]ACC
              933  GGTGACC             
BstPI          51  GGTAACC              G^GT[ACGT]ACC
              933  GGTGACC             
EcoO65I        51  GGTAACC              G^GT[ACGT]ACC
              933  GGTGACC             
FauI           72  CCCGC                CCCGC(4/6)
              177  GCGGG               
HapII          82  CCGG                 C^CGG
              351  CCGG                
HpaII          82  CCGG                 C^CGG
              351  CCGG                
HphI          591  GGTGA                GGTGA(8/7)
              933  GGTGA               
MslI          117  CATCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              848  CACCCCAGTG          
MspI           82  CCGG                 C^CGG
              351  CCGG                
PaeR7I        104  CTCGAG               C^TCGAG
              371  CTCGAG              
TaqI          105  TCGA                 T^CGA
              372  TCGA                
TspRI         420  CACTG                CA[GC]TG[ACGT][ACGT]^
              853  CAGTG               
TthHB8I       105  TCGA                 T^CGA
              372  TCGA                
XhoI          104  CTCGAG               C^TCGAG
              371  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AluI        AlwI        BalI        BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EaeI        EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        MaeIII      MboI        MflI        MluNI       
MnlI        MscI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
SmlI        TseI        Tsp45I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com