Restriction map for pF1KB8255
 (Linear) (Six-base) MAPSORT of: KIBB8255.seq  Check: 734  from: 1  to: 951



KIBB8255.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     921     951 
   Size:        921      30
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     705     829     951 
   Size:        571     134     124     122

  Fragments arranged by size: 

                 571     134     124     122
 
AleI CACnn'nnGTG

Cuts at:      0     852     951 
   Size:        852      99
 
AlwNI CAG_nnn'CTG

Cuts at:      0     891     951 
   Size:        891      60
 
ApaLI G'TGCA_C

Cuts at:      0     855     951 
   Size:        855      96
 
ApoI r'AATT_y

Cuts at:      0     158     951 
   Size:        158     793
 
AvaI C'yCGr_G

Cuts at:      0     104     371     951 
   Size:        104     267     580

  Fragments arranged by size: 

                 580     267     104
 
BamHI G'GATC_C

Cuts at:      0     610     951 
   Size:        610     341
 
BanI G'GyrC_C

Cuts at:      0      25     283     951 
   Size:         25     258     668

  Fragments arranged by size: 

                 668     258      25
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     559     951 
   Size:        559     392
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     575     951 
   Size:         44     531     376

  Fragments arranged by size: 

                 531     376      44
 
BglII A'GATC_T

Cuts at:      0     770     951 
   Size:        770     181
 
Bme1580I G_kGCm'C

Cuts at:      0     286     859     951 
   Size:        286     573      92

  Fragments arranged by size: 

                 573     286      92
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     846     951 
   Size:        846     105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     456     951 
   Size:        456     495
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     587     647     951 
   Size:        587      60     304

  Fragments arranged by size: 

                 587     304      60
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      16     951 
   Size:         16     935
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     139     951 
   Size:        139     812
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     585     615     951 
   Size:        585      30     336

  Fragments arranged by size: 

                 585     336      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     204     330     951 
   Size:        204     126     621

  Fragments arranged by size: 

                 621     204     126
 
BseYI C'CCAG_C

Cuts at:      0      47     506     760     951 
   Size:         47     459     254     191

  Fragments arranged by size: 

                 459     254     191      47
 
BsiHKAI G_wGCw'C

Cuts at:      0     174     859     951 
   Size:        174     685      92

  Fragments arranged by size: 

                 685     174      92
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     501     951 
   Size:        501     450
 
Bsp1286I G_dGCh'C

Cuts at:      0     174     286     859     951 
   Size:        174     112     573      92

  Fragments arranged by size: 

                 573     174     112      92
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     921     951 
   Size:        921      30
 
BsrFI r'CCGG_y

Cuts at:      0     350     951 
   Size:        350     601
 
BssSI C'ACGA_G

Cuts at:      0     291     951 
   Size:        291     660
 
BstEII G'GTnAC_C

Cuts at:      0      51     933     951 
   Size:         51     882      18

  Fragments arranged by size: 

                 882      51      18
 
BstYI r'GATC_y

Cuts at:      0     270     367     610     657     770     951 
   Size:        270      97     243      47     113     181

  Fragments arranged by size: 

                 270     243     181     113      97      47
 
DraIII CAC_nnn'GTG

Cuts at:      0     169     951 
   Size:        169     782
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     517     951 
   Size:        517     434
 
EaeI y'GGCC_r

Cuts at:      0     222     567     639     882     951 
   Size:        222     345      72     243      69

  Fragments arranged by size: 

                 345     243     222      72      69
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      87     951 
   Size:         87     864
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     587     647     705     829     951 
   Size:        571      16      60      58     124     122

  Fragments arranged by size: 

                 571     124     122      60      58      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     757     951 
   Size:        757     194
 
EcoRI G'AATT_C

Cuts at:      0     158     951 
   Size:        158     793
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     436     468     585     617     951 
   Size:        436      32     117      32     334

  Fragments arranged by size: 

                 436     334     117      32      32
 
MscI TGG'CCA

Cuts at:      0     224     569     641     884     951 
   Size:        224     345      72     243      67

  Fragments arranged by size: 

                 345     243     224      72      67
 
MslI CAynn'nnrTG

Cuts at:      0     121     852     951 
   Size:        121     731      99

  Fragments arranged by size: 

                 731     121      99
 
PasI CC'CwG_GG

Cuts at:      0     402     951 
   Size:        402     549
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     653     951 
   Size:        653     298
 
PfoI T'CCnGG_A

Cuts at:      0      80     425     951 
   Size:         80     345     526

  Fragments arranged by size: 

                 526     345      80
 
SexAI A'CCwGG_T

Cuts at:      0     914     951 
   Size:        914      37
 
SmlI C'TyrA_G

Cuts at:      0      31     104     371     951 
   Size:         31      73     267     580

  Fragments arranged by size: 

                 580     267      73      31
 
StyI C'CwwG_G

Cuts at:      0     314     951 
   Size:        314     637
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     394     438     951 
   Size:        394      44     513

  Fragments arranged by size: 

                 513     394      44
 
XhoI C'TCGA_G

Cuts at:      0     104     371     951 
   Size:        104     267     580

  Fragments arranged by size: 

                 580     267     104
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI    ApaLI     ApoI     AvaI    BamHI
     BanI     BbsI     BglI    BglII Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BsrFI    BssSI   BstEII    BstYI   DraIII     DrdI     EaeI
     EciI  Eco57MI EcoO109I    EcoRI    Hin4I     MscI     MslI     PasI
    PflMI     PfoI    SexAI     SmlI     StyI    TaqII     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     EagI     EarI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI
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