Restriction map for pF1KB8244
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BanII       
BbrPI       BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       FauI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MaeIII      MamI        MfeI        MflI        
MluI        MroI        MslI        MunI        MvnI        
NaeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AvaI          314  CCCGAG               C^[CT]CG[GA]G
AvaII         595  GGACC                G^G[AT]CC
BalI           44  TGGCCA               TGG^CCA
BanI          550  GGCGCC               G^G[CT][GA]CC
BbeI          550  GGCGCC               GGCGC^C
BbsI          889  GAAGAC               GAAGAC(2/6)
BclI          269  TGATCA               T^GATCA
BcnI           11  CCGGG                CC^[GC]GG
BglI          416  GCCACCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          303  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         889  GAAGAC               GAAGAC(2/6)
BsmFI         840  GGGAC                GGGAC(10/14)
BsoBI         314  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      303  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      645  TGTACA               T^GTACA
BspDI          73  ATCGAT               AT^CGAT
BsrBI          15  GAGCGG               CCGCTC(-3/-3)
BsrGI         645  TGTACA               T^GTACA
BstF5I        255  GGATG                GGATG(2/0)
CfoI          551  GCGC                 GCG^C
ClaI           73  ATCGAT               AT^CGAT
DraII         594  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI          945  CCATGG               C^C[GA][CT]GG
EcoNI         586  CCTGATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      594  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       945  CCATGG               C^C[AT][AT]GG
EcoT22I       199  ATGCAT               ATGCA^T
FbaI          269  TGATCA               T^GATCA
FokI          255  GGATG                GGATG(9/13)
HaeII         550  GGCGCC               [GA]GCGC^[CT]
HapII          11  CCGG                 C^CGG
HgaI            7  GCGTC                GACGC(5/10)
HhaI          551  GCGC                 GCG^C
HinP1I        551  GCGC                 G^CGC
HinfI         543  GAATC                G^A[ACGT]TC
HpaII          11  CCGG                 C^CGG
KasI          550  GGCGCC               G^GCGCC
MaeII         745  ACGT                 A^CGT
MluNI          44  TGGCCA               TGG^CCA
MscI           44  TGGCCA               TGG^CCA
MspI           11  CCGG                 C^CGG
NarI          550  GGCGCC               GG^CGCC
NciI           11  CCGGG                CC^[GC]GG
NcoI          945  CCATGG               C^CATGG
NdeI          338  CATATG               CA^TATG
NsiI          199  ATGCAT               ATGCA^T
PpuMI         594  AGGACCT              [GA]G^G[AT]CC[CT]
SfoI          550  GGCGCC               GGC^GCC
SmlI          712  CTTGAG               C^T[CT][GA]AG
SphI          201  GCATGC               GCATG^C
SspBI         645  TGTACA               T^GTACA
SspI          659  AATATT               AAT^ATT
StyI          945  CCATGG               C^C[AT][AT]GG
TaqI           74  TCGA                 T^CGA
TfiI          543  GAATC                G^A[AT]TC
TthHB8I        74  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI            6  GGCGTC               G[GA]^CG[CT]C
              550  GGCGCC              
BfaI          244  CTAG                 C^TAG
              758  CTAG                
BsaHI           6  GGCGTC               G[GA]^CG[CT]C
              550  GGCGCC              
Cfr13I         24  GGCCC                G^G[ACGT]CC
              595  GGACC               
EaeI           44  TGGCCA               [CT]^GGCC[GA]
              381  TGGCCG              
Hin1I           6  GGCGTC               G[GA]^CG[CT]C
              550  GGCGCC              
MaeI          244  CTAG                 C^TAG
              758  CTAG                
MspA1I        835  CAGCTG               C[AC]G^C[GT]G
              918  CAGCTG              
NlaIV         486  GGAACC               GG[ACGT]^[ACGT]CC
              550  GGCGCC              
NspI           53  ACATGC               [GA]CATG^[CT]
              201  GCATGC              
PvuII         835  CAGCTG               CAG^CTG
              918  CAGCTG              
Sau96I         24  GGCCC                G^G[ACGT]CC
              595  GGACC               
ScaI          158  AGTACT               AGT^ACT
              457  AGTACT              
Tsp509I       707  AATT                 ^AATT
              990  AATT                
XcmI          266  CCATGATCATTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              417  CCACCCTGGCTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstNI       Cac8I       DdeI        DpnII       
EcoRII      Fnu4HI      HaeIII      HphI        ItaI        
MboI        MboII       MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
ScrFI       SfaNI       Tru9I       TseI        TspRI       
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