Restriction map for pF1KB8242
 (Linear) (Six-base) MAPSORT of: KIBB8242.seq  Check: 7652  from: 1  to: 594



KIBB8242.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AccI GT'mk_AC

Cuts at:      0     359     594 
   Size:        359     235
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     164     594 
   Size:        164     430
 
ApoI r'AATT_y

Cuts at:      0     280     423     552     594 
   Size:        280     143     129      42

  Fragments arranged by size: 

                 280     143     129      42
 
AvaI C'yCGr_G

Cuts at:      0       9     594 
   Size:          9     585
 
BamHI G'GATC_C

Cuts at:      0      96     594 
   Size:         96     498
 
BanI G'GyrC_C

Cuts at:      0      22     594 
   Size:         22     572
 
BanII G_rGCy'C

Cuts at:      0      63     504     594 
   Size:         63     441      90

  Fragments arranged by size: 

                 441      90      63
 
BbeI G_GCGC'C

Cuts at:      0      26     594 
   Size:         26     568
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     528     594 
   Size:        528      66
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     190     522     594 
   Size:        190     332      72

  Fragments arranged by size: 

                 332     190      72
 
BlpI GC'TnA_GC

Cuts at:      0     536     594 
   Size:        536      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     235     333     594 
   Size:        212      23      98     261

  Fragments arranged by size: 

                 261     212      98      23
 
BsaHI Gr'CG_yC

Cuts at:      0      23     594 
   Size:         23     571
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     504     594 
   Size:         63     441      90

  Fragments arranged by size: 

                 441      90      63
 
BspHI T'CATG_A

Cuts at:      0     584     594 
   Size:        584      10
 
BsrDI GCAATG_nn'

Cuts at:      0     185     594 
   Size:        185     409
 
BsrFI r'CCGG_y

Cuts at:      0      25     224     594 
   Size:         25     199     370

  Fragments arranged by size: 

                 370     199      25
 
BstBI TT'CG_AA

Cuts at:      0     519     594 
   Size:        519      75
 
BstYI r'GATC_y

Cuts at:      0      96     594 
   Size:         96     498
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     462     594 
   Size:        462     132
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     353     388     594 
   Size:        353      35     206

  Fragments arranged by size: 

                 353     206      35
 
DraI TTT'AAA

Cuts at:      0     285     594 
   Size:        285     309
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     212     235     333     594 
   Size:        212      23      98     261

  Fragments arranged by size: 

                 261     212      98      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0      70     381     594 
   Size:         70     311     213

  Fragments arranged by size: 

                 311     213      70
 
EcoRI G'AATT_C

Cuts at:      0     423     594 
   Size:        423     171
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     480     594 
   Size:        480     114
 
HaeII r_GCGC'y

Cuts at:      0      26     594 
   Size:         26     568
 
KasI G'GCGC_C

Cuts at:      0      22     594 
   Size:         22     572
 
MfeI C'AATT_G

Cuts at:      0     428     594 
   Size:        428     166
 
MspA1I CmG'CkG

Cuts at:      0     102     594 
   Size:        102     492
 
NaeI GCC'GGC

Cuts at:      0      27     226     594 
   Size:         27     199     368

  Fragments arranged by size: 

                 368     199      27
 
NarI GG'CG_CC

Cuts at:      0      23     594 
   Size:         23     571
 
NdeI CA'TA_TG

Cuts at:      0     561     594 
   Size:        561      33
 
NgoMIV G'CCGG_C

Cuts at:      0      25     224     594 
   Size:         25     199     370

  Fragments arranged by size: 

                 370     199      25
 
PfoI T'CCnGG_A

Cuts at:      0      63      81     594 
   Size:         63      18     513

  Fragments arranged by size: 

                 513      63      18
 
SfcI C'TryA_G

Cuts at:      0     122     594 
   Size:        122     472
 
SfoI GGC'GCC

Cuts at:      0      24     594 
   Size:         24     570
 
SgrAI Cr'CCGG_yG

Cuts at:      0      25     594 
   Size:         25     569
 
SmaI CCC'GGG

Cuts at:      0      11     594 
   Size:         11     583
 
TatI w'GTAC_w

Cuts at:      0     300     594 
   Size:        300     294
 
Tth111I GACn'n_nGTC

Cuts at:      0     177     594 
   Size:        177     417
 
XmaI C'CCGG_G

Cuts at:      0       9     594 
   Size:          9     585
 

 Enzymes that do cut:

     AccI     AlfI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BlpI     BpmI    BsaHI Bsp1286I    BspHI    BsrDI
    BsrFI    BstBI    BstYI    BtgZI    CspCI     DraI  Eco57MI EcoO109I
    EcoRI     FalI    HaeII     KasI     MfeI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     PfoI     SfcI     SfoI    SgrAI     SmaI     TatI
  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MluI     MmeI     MscI
     MslI     NcoI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SgfI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI
     XmnI     ZraI
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