Restriction map for pF1KB8236
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsgI        BsiEI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       BspDI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HphI        
KasI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MluNI       MscI        MspA1I      
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AccIII        827  TCCGGA               T^CCGGA
AciI          423  GCGG                 CCGC(-3/-1)
AcsI          799  AAATTC               [GA]^AATT[CT]
AfaI          247  GTAC                 GT^AC
AocI           33  CCTGAGG              CC^T[ACGT]AGG
ApoI          799  AAATTC               [GA]^AATT[CT]
Asp700I       819  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         115  GAGCAC               G[AT]GC[AT]^C
AvaII         718  GGACC                G^G[AT]CC
BanII          27  GAGCCC               G[GA]GC[CT]^C
BbsI          733  GAAGAC               GAAGAC(2/6)
BbvI          475  GCTGC                GCAGC(8/12)
BfaI          722  CTAG                 C^TAG
BglII         855  AGATCT               A^GATCT
BpuAI         733  GAAGAC               GAAGAC(2/6)
BsaWI         827  TCCGGA               [AT]^CCGG[AT]
BseAI         827  TCCGGA               T^CCGGA
BsiHKAI       115  GAGCAC               G[AT]GC[AT]^C
BsiYI         433  CCTGGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          433  CCTGGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         876  GTCTC                GTCTC(1/5)
Bsp1407I      246  TGTACA               T^GTACA
BspEI         827  TCCGGA               T^CCGGA
BsrDI         190  GCAATG               GCAATG(2/0)
BsrGI         246  TGTACA               T^GTACA
BssKI         433  CCTGG                ^CC[ACGT]GG
BstNI         433  CCTGG                CC^[AT]GG
BstYI         855  AGATCT               [GA]^GATC[CT]
Bsu36I         33  CCTGAGG              CC^T[ACGT]AGG
Cac8I         753  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          568  GCGC                 GCG^C
Eco81I         33  CCTGAGG              CC^T[ACGT]AGG
EcoRII        433  CCTGG                ^CC[AT]GG
EcoT14I       264  CCTTGG               C^C[AT][AT]GG
Fnu4HI        475  GCTGC                GC^[ACGT]GC
HaeII         567  AGCGCC               [GA]GCGC^[CT]
HapII         828  CCGG                 C^CGG
HhaI          568  GCGC                 GCG^C
HinP1I        568  GCGC                 G^CGC
HpaII         828  CCGG                 C^CGG
ItaI          475  GCTGC                GC^[ACGT]GC
MaeI          722  CTAG                 C^TAG
MaeIII        220  GTAAC                ^GT[ACGT]AC
MflI          855  AGATCT               [GA]^GATC[CT]
MroI          827  TCCGGA               T^CCGGA
MspI          828  CCGG                 C^CGG
MvaI          433  CCTGG                CC^[AT]GG
MwoI          601  GCCAAAAATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PpuMI         717  AGGACCT              [GA]G^G[AT]CC[CT]
RsaI          247  GTAC                 GT^AC
ScrFI         433  CCTGG                CC^[ACGT]GG
SfaNI         364  GATGC                GCATC(5/9)
SfcI          728  CTGTAG               C^T[GA][CT]AG
SspBI         246  TGTACA               T^GTACA
StyI          264  CCTTGG               C^C[AT][AT]GG
TaqI           10  TCGA                 T^CGA
TseI          475  GCTGC                G^C[AT]GC
TthHB8I        10  TCGA                 T^CGA
XhoII         855  AGATCT               [GA]^GATC[CT]
XmnI          819  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflII         297  CTTAAG               C^TTAAG
              405  CTTAAG              
AluI          351  AGCT                 AG^CT
              555  AGCT                
BfrI          297  CTTAAG               C^TTAAG
              405  CTTAAG              
BmyI           27  GAGCCC               G[AGT]GC[ACT]^C
              115  GAGCAC              
BpmI          596  CTCCAG               CTGGAG(16/14)
              434  CTGGAG              
BsaJI         264  CCTTGG               C^C[ACGT][ACGT]GG
              432  CCCTGG              
BseRI         385  GAGGAG               GAGGAG(10/8)
              388  GAGGAG              
Bsp1286I       27  GAGCCC               G[AGT]GC[ACT]^C
              115  GAGCAC              
DdeI           34  CTGAG                C^T[ACGT]AG
              345  CTTAG               
DpnII         662  GATC                 ^GATC
              856  GATC                
DraII          37  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              717  AGGACCT             
EcoO109I       37  AGGCCCC              [GA]G^G[ACGT]CC[CT]
              717  AGGACCT             
MboI          662  GATC                 ^GATC
              856  GATC                
MslI           18  CATGGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              328  CATATTAGTG          
NdeII         662  GATC                 ^GATC
              856  GATC                
NlaIII         18  CATG                 CATG^
               24  CATG                
NlaIV          38  GGCCCC               GG[ACGT]^[ACGT]CC
              450  GGTTCC              
PleI          637  GACTC                GAGTC(4/5)
              370  GAGTC               
Sau3AI        662  GATC                 ^GATC
              856  GATC                
SmlI          297  CTTAAG               C^T[CT][GA]AG
              405  CTTAAG              
TfiI            7  GATTC                G^A[AT]TC
              676  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

BsrI        BstF5I      Cfr13I      EarI        FokI        
HaeIII      HinfI       Ksp632I     MboII       MnlI        
MseI        Sau96I      Tru9I       Tsp509I     TspRI       
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