Restriction map for pF1KB8231
##################################
# NO CUT ENZYMES (  151 enzymes) #
##################################

AatII       AccI        AccII       AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BalI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsgI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
Ksp632I     KspI        MaeII       MaeIII      MluI        
MluNI       MscI        MseI        MspA1I      MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tru9I       Tsp45I      Van91I      XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

Acc65I        335  GGTACC               G^GTACC
AccIII        453  TCCGGA               T^CCGGA
AciI          534  GCGG                 CCGC(-3/-1)
AfaI          336  GTAC                 GT^AC
AocI          310  CCTAAGG              CC^T[ACGT]AGG
ApaI          207  GGGCCC               GGGCC^C
Asp700I       238  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       335  GGTACC               G^GTACC
AspHI         230  GAGCTC               G[AT]GC[AT]^C
AspI           97  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          167  CCCGAG               C^[CT]CG[GA]G
BamHI         624  GGATCC               G^GATCC
BanI          335  GGTACC               G^G[CT][GA]CC
BbvI          126  GCTGC                GCAGC(8/12)
BfaI          388  CTAG                 C^TAG
BsaBI         619  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI          85  CCCCGG               C^C[ACGT][ACGT]GG
BsaWI         453  TCCGGA               [AT]^CCGG[AT]
BseAI         453  TCCGGA               T^CCGGA
BsiHKAI       230  GAGCTC               G[AT]GC[AT]^C
BsmBI         525  GAGACG               CGTCTC(1/5)
BsmFI         598  GTCCC                GGGAC(10/14)
BsoBI         167  CCCGAG               C^[CT]CG[GA]G
BspEI         453  TCCGGA               T^CCGGA
BspHI         234  TCATGA               T^CATGA
BsrDI         156  CATTGC               GCAATG(2/0)
BstXI         275  CCAACATCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         624  GGATCC               [GA]^GATC[CT]
Bsu36I        310  CCTAAGG              CC^T[ACGT]AGG
Cac8I          48  GCTGGC               GC[ACGT]^[ACGT]GC
Eco81I        310  CCTAAGG              CC^T[ACGT]AGG
EcoRV         115  GATATC               GAT^ATC
Fnu4HI        126  GCTGC                GC^[ACGT]GC
HincII        291  GTTGAC               GT[CT]^[GA]AC
HindII        291  GTTGAC               GT[CT]^[GA]AC
HphI          186  TCACC                GGTGA(8/7)
ItaI          126  GCTGC                GC^[ACGT]GC
KpnI          335  GGTACC               GGTAC^C
MaeI          388  CTAG                 C^TAG
MamI          619  GATAAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         507  GAAGA                GAAGA(8/7)
MfeI          148  CAATTG               C^AATTG
MflI          624  GGATCC               [GA]^GATC[CT]
MroI          453  TCCGGA               T^CCGGA
MslI           53  CATCTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          148  CAATTG               C^AATTG
PflFI          97  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           63  GACTC                GAGTC(4/5)
PpuMI         255  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI          407  CTGCAG               CTGCA^G
RcaI          234  TCATGA               T^CATGA
RsaI          336  GTAC                 GT^AC
SacI          230  GAGCTC               GAGCT^C
SfcI          407  CTGCAG               C^T[GA][CT]AG
TaqI          420  TCGA                 T^CGA
TfiI          198  GAATC                G^A[AT]TC
TseI          126  GCTGC                G^C[AT]GC
Tsp509I       149  AATT                 ^AATT
TspRI         405  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        97  GACCGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       420  TCGA                 T^CGA
XbaI          387  TCTAGA               T^CTAGA
XhoII         624  GGATCC               [GA]^GATC[CT]
XmnI          238  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          183  AGCT                 AG^CT
              231  AGCT                
AlwNI         121  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              565  CAGAGACTG           
AvaII          89  GGACC                G^G[AT]CC
              256  GGTCC               
BanII         207  GGGCCC               G[GA]GC[CT]^C
              230  GAGCTC              
BcnI           86  CCCGG                CC^[GC]GG
               99  CCGGG               
BmyI          207  GGGCCC               G[AGT]GC[ACT]^C
              230  GAGCTC              
BseRI         518  GAGGAG               GAGGAG(10/8)
              537  GAGGAG              
Bsp1286I      207  GGGCCC               G[AGT]GC[ACT]^C
              230  GAGCTC              
DdeI          105  CTCAG                C^T[ACGT]AG
              311  CTAAG               
HinfI          63  GACTC                G^A[ACGT]TC
              198  GAATC               
MwoI          184  GCTCACCAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              209  GCCCCCAGAGC         
NciI           86  CCCGG                CC^[GC]GG
               99  CCGGG               
SfaNI          52  GCATC                GCATC(5/9)
              441  GCATC               
XcmI          161  CCAGGCCCCGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              194  CCATGAATCTTGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AlwI        BpmI        BsaI        BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
FokI        HaeIII      HapII       HpaII       MboI        
MnlI        MspI        MvaI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       
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