Restriction map for pF1KB8230
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaBI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      CpoI        DraI        
DraII       DraIII      DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        Fnu4HI      FseI        FspI        
HaeII       ItaI        KasI        KpnI        KspI        
MaeI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccI         1009  GTCTAC               GT^[AC][GT]AC
AseI          148  ATTAAT               AT^TAAT
AsnI          148  ATTAAT               AT^TAAT
BanI          981  GGCACC               G^G[CT][GA]CC
BbrPI         236  CACGTG               CAC^GTG
BcnI          632  CCCGG                CC^[GC]GG
BpmI          502  CTCCAG               CTGGAG(16/14)
BsaAI         236  CACGTG               [CT]AC^GT[GA]
BsaI         1016  GAGACC               GGTCTC(1/5)
BspDI         136  ATCGAT               AT^CGAT
BssSI         656  CACGAG               CACGAG(-5/-1)
BstAPI         63  GCACGCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1020  CCAGCCATATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           72  GCGC                 GCG^C
ClaI          136  ATCGAT               AT^CGAT
DrdI          276  GACAAAAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          634  CGGCCA               [CT]^GGCC[GA]
HapII         633  CCGG                 C^CGG
HhaI           72  GCGC                 GCG^C
HinP1I         72  GCGC                 G^CGC
HincII        510  GTTAAC               GT[CT]^[GA]AC
HindII        510  GTTAAC               GT[CT]^[GA]AC
HindIII       416  AAGCTT               A^AGCTT
HpaI          510  GTTAAC               GTT^AAC
HpaII         633  CCGG                 C^CGG
MaeII         237  ACGT                 A^CGT
MaeIII        625  GTTAC                ^GT[ACGT]AC
MspI          633  CCGG                 C^CGG
NciI          632  CCCGG                CC^[GC]GG
NdeI         1025  CATATG               CA^TATG
NspI          435  ACATGC               [GA]CATG^[CT]
PmaCI         236  CACGTG               CAC^GTG
PmlI          236  CACGTG               CAC^GTG
PshBI         148  ATTAAT               AT^TAAT
PstI          410  CTGCAG               CTGCA^G
ScaI          356  AGTACT               AGT^ACT
SmlI          969  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          772  CCGC                 CCGC(-3/-1)
               31  GCGG                
AcyI          663  GACGCC               G[GA]^CG[CT]C
             1006  GGCGTC              
Asp700I        94  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              354  GAAGTACTTC          
AvaI           19  CCCGAG               C^[CT]CG[GA]G
              692  CCCGAG              
AvaII          48  GGACC                G^G[AT]CC
              232  GGTCC               
BsaHI         663  GACGCC               G[GA]^CG[CT]C
             1006  GGCGTC              
BsoBI          19  CCCGAG               C^[CT]CG[GA]G
              692  CCCGAG              
BspMI          14  ACCTGC               ACCTGC(4/8)
              408  ACCTGC              
Cac8I          63  GCACGC               GC[ACGT]^[ACGT]GC
              492  GCAAGC              
DdeI          203  CTAAG                C^T[ACGT]AG
              922  CTCAG               
DpnII         376  GATC                 ^GATC
              486  GATC                
EcoT14I       291  CCTTGG               C^C[AT][AT]GG
              320  CCAAGG              
HgaI          663  GACGC                GACGC(5/10)
             1007  GCGTC               
Hin1I         663  GACGCC               G[GA]^CG[CT]C
             1006  GGCGTC              
HphI           78  GGTGA                GGTGA(8/7)
              144  GGTGA               
MboI          376  GATC                 ^GATC
              486  GATC                
NdeII         376  GATC                 ^GATC
              486  GATC                
Sau3AI        376  GATC                 ^GATC
              486  GATC                
StyI          291  CCTTGG               C^C[AT][AT]GG
              320  CCAAGG              
TaqI          137  TCGA                 T^CGA
              558  TCGA                
TfiI          853  GATTC                G^A[AT]TC
              940  GATTC               
Tsp509I       183  AATT                 ^AATT
              244  AATT                
TthHB8I       137  TCGA                 T^CGA
              558  TCGA                
XmnI           94  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              354  GAAGTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsrI        BssKI       
BstF5I      BstNI       Cfr13I      EarI        EcoRII      
FokI        HaeIII      HinfI       Ksp632I     MboII       
MnlI        MseI        MslI        MvaI        MwoI        
NlaIII      NlaIV       PleI        RsaI        Sau96I      
ScrFI       SfcI        Tru9I       TspRI       
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