Restriction map for pF1KB8230
 (Linear) (Six-base) MAPSORT of: KIBB8230.seq  Check: 9259  from: 1  to: 1038



KIBB8230.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     417    1038 
   Size:        417     621
 
AccI GT'mk_AC

Cuts at:      0    1010    1038 
   Size:       1010      28
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     971    1038 
   Size:        271     700      67

  Fragments arranged by size: 

                 700     271      67
 
AseI AT'TA_AT

Cuts at:      0     149    1038 
   Size:        149     889
 
AvaI C'yCGr_G

Cuts at:      0      19     692    1038 
   Size:         19     673     346

  Fragments arranged by size: 

                 673     346      19
 
BanI G'GyrC_C

Cuts at:      0     981    1038 
   Size:        981      57
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     764    1038 
   Size:        764     274
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      57     739    1038 
   Size:         57     682     299

  Fragments arranged by size: 

                 682     299      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487    1038 
   Size:        487     551
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     954    1038 
   Size:        954      84
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1010    1038 
   Size:       1010      28
 
BsaAI yAC'GTr

Cuts at:      0     238    1038 
   Size:        238     800
 
BsaHI Gr'CG_yC

Cuts at:      0     664    1007    1038 
   Size:        664     343      31

  Fragments arranged by size: 

                 664     343      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      25     635     654     665     684    1038 
   Size:         25     610      19      11      19     354

  Fragments arranged by size: 

                 610     354      25      19      19      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     635     692     755     943    1038 
   Size:         19     616      57      63     188      95

  Fragments arranged by size: 

                 616     188      95      63      57      19
 
BseYI C'CCAG_C

Cuts at:      0     165     597     879    1038 
   Size:        165     432     282     159

  Fragments arranged by size: 

                 432     282     165     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      23     417    1038 
   Size:         23     394     621

  Fragments arranged by size: 

                 621     394      23
 
BssSI C'ACGA_G

Cuts at:      0     656    1038 
   Size:        656     382
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      69    1038 
   Size:         69     969
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1027    1038 
   Size:       1027      11
 
BtsI GCAGTG_nn'

Cuts at:      0     827    1038 
   Size:        827     211
 
ClaI AT'CG_AT

Cuts at:      0     137    1038 
   Size:        137     901
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     940     975    1038 
   Size:        940      35      63

  Fragments arranged by size: 

                 940      63      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     282    1038 
   Size:        282     756
 
EaeI y'GGCC_r

Cuts at:      0     634    1038 
   Size:        634     404
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     708     950    1038 
   Size:        323     385     242      88

  Fragments arranged by size: 

                 385     323     242      88
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      46    1038 
   Size:         46     992
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     487     971    1038 
   Size:        271     216     484      67

  Fragments arranged by size: 

                 484     271     216      67
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     228    1038 
   Size:        228     810
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     654     686     905     937    1038 
   Size:        654      32     219      32     101

  Fragments arranged by size: 

                 654     219     101      32      32
 
HincII GTy'rAC

Cuts at:      0     512    1038 
   Size:        512     526
 
HindIII A'AGCT_T

Cuts at:      0     416    1038 
   Size:        416     622
 
HpaI GTT'AAC

Cuts at:      0     512    1038 
   Size:        512     526
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     621    1038 
   Size:        621     417
 
MslI CAynn'nnrTG

Cuts at:      0     139     368     394    1038 
   Size:        139     229      26     644

  Fragments arranged by size: 

                 644     229     139      26
 
NdeI CA'TA_TG

Cuts at:      0    1026    1038 
   Size:       1026      12
 
NspI r_CATG'y

Cuts at:      0     439    1038 
   Size:        439     599
 
PfoI T'CCnGG_A

Cuts at:      0     503    1038 
   Size:        503     535
 
PmlI CAC'GTG

Cuts at:      0     238    1038 
   Size:        238     800
 
PsiI TTA'TAA

Cuts at:      0     476    1038 
   Size:        476     562
 
PstI C_TGCA'G

Cuts at:      0     414    1038 
   Size:        414     624
 
ScaI AGT'ACT

Cuts at:      0     358    1038 
   Size:        358     680
 
SfcI C'TryA_G

Cuts at:      0     410     870    1011    1038 
   Size:        410     460     141      27

  Fragments arranged by size: 

                 460     410     141      27
 
SmlI C'TyrA_G

Cuts at:      0     969    1038 
   Size:        969      69
 
StyI C'CwwG_G

Cuts at:      0     291     320    1038 
   Size:        291      29     718

  Fragments arranged by size: 

                 718     291      29
 
TatI w'GTAC_w

Cuts at:      0     131     356    1038 
   Size:        131     225     682

  Fragments arranged by size: 

                 682     225     131
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     161     193    1038 
   Size:        161      32     845

  Fragments arranged by size: 

                 845     161      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      98     358    1038 
   Size:         98     260     680

  Fragments arranged by size: 

                 680     260      98
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AseI     AvaI     BanI     BmrI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
    BspMI    BssSI   BstAPI    BstXI     BtsI     ClaI    CspCI     DrdI
     EaeI     EarI     EciI  Eco57MI     FalI    Hin4I   HincII  HindIII
     HpaI     MmeI     MslI     NdeI     NspI     PfoI     PmlI     PsiI
     PstI     ScaI     SfcI     SmlI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI   Bpu10I    BsaBI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     DraI   DraIII     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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