Restriction map for pF1KB8229
 (Linear) (Six-base) MAPSORT of: KIBB8229.seq  Check: 1013  from: 1  to: 504



KIBB8229.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AfeI AGC'GCT

Cuts at:      0     325     504 
   Size:        325     179
 
ApaI G_GGCC'C

Cuts at:      0      70      92     504 
   Size:         70      22     412

  Fragments arranged by size: 

                 412      70      22
 
BanI G'GyrC_C

Cuts at:      0     297     504 
   Size:        297     207
 
BanII G_rGCy'C

Cuts at:      0      70      92     269     341     504 
   Size:         70      22     177      72     163

  Fragments arranged by size: 

                 177     163      72      70      22
 
BbeI G_GCGC'C

Cuts at:      0     301     504 
   Size:        301     203
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      44     441     504 
   Size:         44     397      63

  Fragments arranged by size: 

                 397      63      44
 
Bme1580I G_kGCm'C

Cuts at:      0      70      92     409     504 
   Size:         70      22     317      95

  Fragments arranged by size: 

                 317      95      70      22
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     417     504 
   Size:         27     390      87

  Fragments arranged by size: 

                 390      87      27
 
BsaHI Gr'CG_yC

Cuts at:      0     140     298     504 
   Size:        140     158     206

  Fragments arranged by size: 

                 206     158     140
 
BsaWI w'CCGG_w

Cuts at:      0     206     341     504 
   Size:        206     135     163

  Fragments arranged by size: 

                 206     163     135
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     336     366     504 
   Size:        336      30     138

  Fragments arranged by size: 

                 336     138      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     277     504 
   Size:        277     227
 
BseYI C'CCAG_C

Cuts at:      0      70     190     268     504 
   Size:         70     120      78     236

  Fragments arranged by size: 

                 236     120      78      70
 
BsiHKAI G_wGCw'C

Cuts at:      0     341     504 
   Size:        341     163
 
Bsp1286I G_dGCh'C

Cuts at:      0      70      92     269     341     409     504 
   Size:         70      22     177      72      68      95

  Fragments arranged by size: 

                 177      95      72      70      68      22
 
BspEI T'CCGG_A

Cuts at:      0     206     341     504 
   Size:        206     135     163

  Fragments arranged by size: 

                 206     163     135
 
BsrBI CCG'CTC

Cuts at:      0     287     504 
   Size:        287     217
 
BstYI r'GATC_y

Cuts at:      0       8     203     504 
   Size:          8     195     301

  Fragments arranged by size: 

                 301     195       8
 
Bsu36I CC'TnA_GG

Cuts at:      0      93     504 
   Size:         93     411
 
BtgI C'CryG_G

Cuts at:      0     230     504 
   Size:        230     274
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      97     504 
   Size:         97     407
 
EaeI y'GGCC_r

Cuts at:      0     330     504 
   Size:        330     174
 
EarI CTCTTCn'nnn_

Cuts at:      0     391     504 
   Size:        391     113
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     480     504 
   Size:        480      24
 
EcoICRI GAG'CTC

Cuts at:      0     339     504 
   Size:        339     165
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     417     504 
   Size:         27     390      87

  Fragments arranged by size: 

                 390      87      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60      67      89     120     504 
   Size:         60       7      22      31     384

  Fragments arranged by size: 

                 384      60      31      22       7
 
HaeII r_GCGC'y

Cuts at:      0     191     301     327     504 
   Size:        191     110      26     177

  Fragments arranged by size: 

                 191     177     110      26
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63      76     108     195     227     504 
   Size:         31      32      13      32      87      32     277

  Fragments arranged by size: 

                 277      87      32      32      32      31      13
 
KasI G'GCGC_C

Cuts at:      0     297     504 
   Size:        297     207
 
MspA1I CmG'CkG

Cuts at:      0     232     437     504 
   Size:        232     205      67

  Fragments arranged by size: 

                 232     205      67
 
NarI GG'CG_CC

Cuts at:      0     298     504 
   Size:        298     206
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     311     504 
   Size:        311     193
 
PshAI GACnn'nnGTC

Cuts at:      0     143     445     504 
   Size:        143     302      59

  Fragments arranged by size: 

                 302     143      59
 
PspOMI G'GGCC_C

Cuts at:      0      66      88     504 
   Size:         66      22     416

  Fragments arranged by size: 

                 416      66      22
 
PstI C_TGCA'G

Cuts at:      0      54     504 
   Size:         54     450
 
PvuII CAG'CTG

Cuts at:      0     437     504 
   Size:        437      67
 
SacI G_AGCT'C

Cuts at:      0     341     504 
   Size:        341     163
 
SacII CC_GC'GG

Cuts at:      0     233     504 
   Size:        233     271
 
SapI GCTCTTCn'nnn_

Cuts at:      0     391     504 
   Size:        391     113
 
SfcI C'TryA_G

Cuts at:      0      50     504 
   Size:         50     454
 
SfoI GGC'GCC

Cuts at:      0     299     504 
   Size:        299     205
 
StuI AGG'CCT

Cuts at:      0     128     504 
   Size:        128     376
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     336     368     504 
   Size:        336      32     136

  Fragments arranged by size: 

                 336     136      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     131     504 
   Size:        131     373
 

 Enzymes that do cut:

     AfeI     ApaI     BanI    BanII     BbeI     BbsI Bme1580I     BpmI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI
    BsrBI    BstYI   Bsu36I     BtgI    BtgZI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I     KasI   MspA1I     NarI
    PflMI    PshAI   PspOMI     PstI    PvuII     SacI    SacII     SapI
     SfcI     SfoI     StuI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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