Restriction map for pF1KB8225
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
FbaI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MroI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tsp45I      
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflIII        128  ACATGT               A^C[GA][CT]GT
AlwI          315  GGATC                GGATC(4/5)
AspHI         115  GAGCTC               G[AT]GC[AT]^C
AvaI           50  CCCGAG               C^[CT]CG[GA]G
BalI          143  TGGCCA               TGG^CCA
BbsI          181  GAAGAC               GAAGAC(2/6)
BbvCI          76  GCTGAGG              CCTCAGC(-5/-2)
BpmI          415  CTGGAG               CTGGAG(16/14)
BpuAI         181  GAAGAC               GAAGAC(2/6)
BsiHKAI       115  GAGCTC               G[AT]GC[AT]^C
BsiYI         153  CCTGACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          153  CCTGACTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI          53  GAGACG               CGTCTC(1/5)
BsmFI         125  GGGAC                GGGAC(10/14)
BsoBI          50  CCCGAG               C^[CT]CG[GA]G
BspLU11I      128  ACATGT               A^CATGT
BspMI         339  GCAGGT               ACCTGC(4/8)
BstF5I        375  GGATG                GGATG(2/0)
BstNI         195  CCTGG                CC^[AT]GG
DsaI           65  CCGTGG               C^C[GA][CT]GG
EarI          334  GAAGAG               CTCTTC(1/4)
EcoRII        195  CCTGG                ^CC[AT]GG
EcoT22I       377  ATGCAT               ATGCA^T
FauI           46  CCCGC                CCCGC(4/6)
FokI          375  GGATG                GGATG(9/13)
HphI          215  TCACC                GGTGA(8/7)
Ksp632I       334  GAAGAG               CTCTTC(1/4)
MluNI         143  TGGCCA               TGG^CCA
MscI          143  TGGCCA               TGG^CCA
MspA1I         74  CAGCTG               C[AC]G^C[GT]G
MvaI          195  CCTGG                CC^[AT]GG
NlaIII        129  CATG                 CATG^
NlaIV          90  GGAGCC               GG[ACGT]^[ACGT]CC
NsiI          377  ATGCAT               ATGCA^T
NspI          128  ACATGT               [GA]CATG^[CT]
PstI          301  CTGCAG               CTGCA^G
PvuII          74  CAGCTG               CAG^CTG
SacI          115  GAGCTC               GAGCT^C
SapI          334  GAAGAGC              GCTCTTC(1/4)
SfcI          301  CTGCAG               C^T[GA][CT]AG
SmlI          352  CTTGAG               C^T[CT][GA]AG
XcmI          285  CCAGAAATATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI           22  GTAC                 GT^AC
              405  GTAC                
BanII          43  GAGCCC               G[GA]GC[CT]^C
              115  GAGCTC              
BcnI           32  CCCGG                CC^[GC]GG
              123  CCGGG               
BmyI           43  GAGCCC               G[AGT]GC[ACT]^C
              115  GAGCTC              
BsaJI          14  CCGAGG               C^C[ACGT][ACGT]GG
               65  CCGTGG              
BsmAI          53  GAGAC                GTCTC(1/5)
               70  GAGAC               
Bsp1286I       43  GAGCCC               G[AGT]GC[ACT]^C
              115  GAGCTC              
DdeI           77  CTGAG                C^T[ACGT]AG
              113  CTGAG               
DpnII         316  GATC                 ^GATC
              321  GATC                
EaeI          143  TGGCCA               [CT]^GGCC[GA]
              170  CGGCCA              
HaeIII        144  GGCC                 GG^CC
              171  GGCC                
HapII          33  CCGG                 C^CGG
              123  CCGG                
HpaII          33  CCGG                 C^CGG
              123  CCGG                
MboI          316  GATC                 ^GATC
              321  GATC                
MboII         181  GAAGA                GAAGA(8/7)
              334  GAAGA               
MnlI           16  GAGG                 CCTC(7/6)
               79  GAGG                
MseI          271  TTAA                 T^TAA
              280  TTAA                
MspI           33  CCGG                 C^CGG
              123  CCGG                
NciI           32  CCCGG                CC^[GC]GG
              123  CCGGG               
NdeII         316  GATC                 ^GATC
              321  GATC                
RsaI           22  GTAC                 GT^AC
              405  GTAC                
Sau3AI        316  GATC                 ^GATC
              321  GATC                
SfaNI          58  GATGC                GCATC(5/9)
              376  GATGC               
Tru9I         271  TTAA                 T^TAA
              280  TTAA                
TspRI         111  CACTG                CA[GC]TG[ACGT][ACGT]^
              177  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

AciI        AluI        BbvI        BsrI        BssKI       
Cac8I       Fnu4HI      ItaI        MwoI        ScrFI       
TseI        
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