Restriction map for pF1KB8221
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AfaI        AflII       AflIII      AgeI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BalI        BbrPI       BbvCI       BcgI        BclI        
BfrI        BglII       BlnI        BpmI        BsaAI       
BsaBI       BsaI        BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CpoI        DraI        DrdI        DsaI        
EagI        EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
HincII      HindII      HpaI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MscI        MslI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StyI        SwaI        TfiI        
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII          51  CGCG                 CG^CG
AccIII        213  TCCGGA               T^CCGGA
AhdI          607  GACGAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        739  GTGCAC               G^TGCAC
ApaI          181  GGGCCC               GGGCC^C
ApaLI         739  GTGCAC               G^TGCAC
AspEI         607  GACGAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         739  GTGCAC               G[AT]GC[AT]^C
AvaI          178  CCCGGG               C^[CT]CG[GA]G
BamHI          12  GGATCC               G^GATCC
BanII         181  GGGCCC               G[GA]GC[CT]^C
BbsI           85  GAAGAC               GAAGAC(2/6)
BciVI         326  GTATCC               GTATCC(6/5)
BglI          100  GCCTTCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI            4  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I        4  GCTGAGC              GC^T[ACGT]AGC
BpuAI          85  GAAGAC               GAAGAC(2/6)
BsaWI         213  TCCGGA               [AT]^CCGG[AT]
BseAI         213  TCCGGA               T^CCGGA
BsiHKAI       739  GTGCAC               G[AT]GC[AT]^C
BsmAI         691  GTCTC                GTCTC(1/5)
BsoBI         178  CCCGGG               C^[CT]CG[GA]G
BspDI         302  ATCGAT               AT^CGAT
BspEI         213  TCCGGA               T^CCGGA
BstUI          51  CGCG                 CG^CG
BstYI          12  GGATCC               [GA]^GATC[CT]
CelII           4  GCTGAGC              GC^T[ACGT]AGC
ClaI          302  ATCGAT               AT^CGAT
DraIII        624  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI           68  TGGCCG               [CT]^GGCC[GA]
Eam1105I      607  GACGAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII       497  AAGCTT               A^AGCTT
HinfI         610  GAGTC                G^A[ACGT]TC
HphI          241  GGTGA                GGTGA(8/7)
MaeII         524  ACGT                 A^CGT
MflI           12  GGATCC               [GA]^GATC[CT]
MroI          213  TCCGGA               T^CCGGA
MseI          659  TTAA                 T^TAA
MspA1I        733  CCGCTG               C[AC]G^C[GT]G
MvnI           51  CGCG                 CG^CG
NlaIII        699  CATG                 CATG^
NspI          698  GCATGC               [GA]CATG^[CT]
PleI          610  GAGTC                GAGTC(4/5)
SgrAI          75  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          178  CCCGGG               CCC^GGG
SmlI          721  CTCAAG               C^T[CT][GA]AG
SphI          698  GCATGC               GCATG^C
StuI          149  AGGCCT               AGG^CCT
Tru9I         659  TTAA                 T^TAA
Tsp509I       379  AATT                 ^AATT
TspRI         624  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII          12  GGATCC               [GA]^GATC[CT]
XmaCI         178  CCCGGG               C^CCGGG
XmaI          178  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwNI         314  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              620  CAGGCACTG           
BmyI          181  GGGCCC               G[AGT]GC[ACT]^C
              739  GTGCAC              
BsmFI          47  GTCCC                GGGAC(10/14)
              615  GTCCC               
Bsp1286I      181  GGGCCC               G[AGT]GC[ACT]^C
              739  GTGCAC              
DpnII          13  GATC                 ^GATC
              301  GATC                
DraII         181  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              728  AGGCCCC             
EcoNI         567  CCTAGAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              720  CCTCAAGCAGG         
EcoO109I      181  GGGCCCC              [GA]G^G[ACGT]CC[CT]
              728  AGGCCCC             
MaeIII        538  GTCAC                ^GT[ACGT]AC
              653  GTGAC               
MboI           13  GATC                 ^GATC
              301  GATC                
NdeII          13  GATC                 ^GATC
              301  GATC                
Sau3AI         13  GATC                 ^GATC
              301  GATC                
SfcI          460  CTACAG               C^T[GA][CT]AG
              561  CTACAG              
TaqI          137  TCGA                 T^CGA
              303  TCGA                
Tsp45I        538  GTCAC                ^GT[GC]AC
              653  GTGAC               
TthHB8I       137  TCGA                 T^CGA
              303  TCGA                
XcmI          252  CCAGGAAAGTAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              619  CCAGGCACTGAGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        AvaII       
BanI        BbeI        BbvI        BcnI        BfaI        
BsaHI       BsaJI       BsiYI       BslI        BsrFI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        Hin1I       HinP1I      HpaII       ItaI        
KasI        MaeI        MboII       MnlI        MspI        
MvaI        MwoI        NaeI        NarI        NciI        
NgoMIV      NlaIV       Sau96I      ScrFI       SfaNI       
SfoI        TseI        
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