Restriction map for pF1KB8207
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

AccI        AccII       AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
AspHI       AspI        AviII       AvrII       BamHI       
BanII       BbeI        BbrPI       BbsI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaI        BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KspI        
MaeI        MamI        MfeI        MluI        MroI        
MseI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       Tth111I     
Van91I      XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         202  GACGTC               GACGT^C
Acc65I        497  GGTACC               G^GTACC
AcsI          241  GAATTC               [GA]^AATT[CT]
AflIII        551  ACATGT               A^C[GA][CT]GT
AhdI          550  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI          241  GAATTC               [GA]^AATT[CT]
Asp718I       497  GGTACC               G^GTACC
AspEI         550  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          443  CTCGAG               C^[CT]CG[GA]G
BalI          584  TGGCCA               TGG^CCA
BbvCI         478  GCTGAGG              CCTCAGC(-5/-2)
BcgI          614  GCAATGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         152  AGATCT               A^GATCT
BmyI          754  GTGCCC               G[AGT]GC[ACT]^C
BsaWI         507  ACCGGA               [AT]^CCGG[AT]
BseRI         670  GAGGAG               GAGGAG(10/8)
BsgI          208  CTGCAC               GTGCAG(16/14)
BsmAI          73  GAGAC                GTCTC(1/5)
BsoBI         443  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      754  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I      551  ACATGT               A^CATGT
BspMI         392  ACCTGC               ACCTGC(4/8)
BsrFI         562  GCCGGC               [GA]^CCGG[CT]
BstEII        286  GGTGACC              G^GT[ACGT]ACC
BstPI         286  GGTGACC              G^GT[ACGT]ACC
Cfr10I        562  GCCGGC               [GA]^CCGG[CT]
DraIII        489  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      550  GACATGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         159  CCTACTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       286  GGTGACC              G^GT[ACGT]ACC
EcoRI         241  GAATTC               G^AATTC
HgaI          660  GACGC                GACGC(5/10)
KpnI          497  GGTACC               GGTAC^C
MluNI         584  TGGCCA               TGG^CCA
MscI          584  TGGCCA               TGG^CCA
MslI          546  CATCGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          562  GCCGGC               GCC^GGC
NgoMIV        562  GCCGGC               G^CCGGC
NspI          551  ACATGT               [GA]CATG^[CT]
PaeR7I        443  CTCGAG               C^TCGAG
PleI           75  GACTC                GAGTC(4/5)
PshAI         862  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         476  CAGCTG               CAG^CTG
SfaNI         569  GCATC                GCATC(5/9)
XcmI          731  CCACAACATCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          443  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          202  GACGTC               G[GA]^CG[CT]C
              660  GACGCC              
AlwNI         253  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              388  CAGAACCTG           
AvaII         255  GGACC                G^G[AT]CC
              375  GGACC               
BanI           37  GGCACC               G^G[CT][GA]CC
              497  GGTACC              
BsaHI         202  GACGTC               G[GA]^CG[CT]C
              660  GACGCC              
BsaJI         668  CCGAGG               C^C[ACGT][ACGT]GG
              740  CCCTGG              
BsmFI         374  GGGAC                GGGAC(10/14)
              658  GGGAC               
BspHI          78  TCATGA               T^CATGA
              198  TCATGA              
BsrI           18  ACTGG                ACTGG(1/-1)
              630  CCAGT               
Cac8I         562  GCCGGC               GC[ACGT]^[ACGT]GC
              582  GCTGGC              
EaeI          584  TGGCCA               [CT]^GGCC[GA]
              728  CGGCCA              
Hin1I         202  GACGTC               G[GA]^CG[CT]C
              660  GACGCC              
MspA1I        465  CCGCTG               C[AC]G^C[GT]G
              476  CAGCTG              
PpuMI         254  AGGACCT              [GA]G^G[AT]CC[CT]
              374  GGGACCT             
PstI          787  CTGCAG               CTGCA^G
              841  CTGCAG              
RcaI           78  TCATGA               T^CATGA
              198  TCATGA              
SfcI          787  CTGCAG               C^T[GA][CT]AG
              841  CTGCAG              
SmlI          163  CTCAAG               C^T[CT][GA]AG
              443  CTCGAG              
TfiI          344  GATTC                G^A[AT]TC
              456  GATTC               
Tsp509I       242  AATT                 ^AATT
              422  AATT                
TspRI         491  CACTG                CA[GC]TG[ACGT][ACGT]^
              676  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BsiYI       BslI        BsrDI       BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       DraII       EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TaqI        TseI        Tsp45I      TthHB8I     
XhoII       
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