Restriction map for pF1KB8201
##################################
# NO CUT ENZYMES (  161 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstEII      BstPI       BstUI       
BstXI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MluI        
MroI        MseI        MslI        MspA1I      MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfcI        SfiI        SfoI        
SgrAI       SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI          259  GTATAC               GT^[AC][GT]AC
AfaI          557  GTAC                 GT^AC
AlwI          138  GATCC                GGATC(4/5)
Asp700I       279  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         597  GTGCTC               G[AT]GC[AT]^C
BbvI           33  GCTGC                GCAGC(8/12)
BcgI          444  CGAAACTAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI           71  TGATCA               T^GATCA
BmyI          597  GTGCTC               G[AGT]GC[ACT]^C
BsgI          391  GTGCAG               GTGCAG(16/14)
BsiHKAI       597  GTGCTC               G[AT]GC[AT]^C
BsmI          324  GAATGC               GAATGC(1/-1)
Bsp1286I      597  GTGCTC               G[AGT]GC[ACT]^C
BsrBI         202  GAGCGG               CCGCTC(-3/-3)
Bst1107I      259  GTATAC               GTA^TAC
BstBI         442  TTCGAA               TT^CGAA
BstYI         137  AGATCC               [GA]^GATC[CT]
BstZ17I       259  GTATAC               GTA^TAC
CfoI          345  GCGC                 GCG^C
Cfr13I        495  GGCCC                G^G[ACGT]CC
EarI           22  CTCTTC               CTCTTC(1/4)
EcoT14I       242  CCATGG               C^C[AT][AT]GG
FbaI           71  TGATCA               T^GATCA
Fnu4HI         33  GCTGC                GC^[ACGT]GC
HaeII         344  AGCGCC               [GA]GCGC^[CT]
HhaI          345  GCGC                 GCG^C
HinP1I        345  GCGC                 G^CGC
ItaI           33  GCTGC                GC^[ACGT]GC
Ksp632I        22  CTCTTC               CTCTTC(1/4)
MflI          137  AGATCC               [GA]^GATC[CT]
NcoI          242  CCATGG               C^CATGG
NlaIV         238  GGAGCC               GG[ACGT]^[ACGT]CC
NspV          442  TTCGAA               TT^CGAA
PaeR7I        419  CTCGAG               C^TCGAG
PleI           46  GACTC                GAGTC(4/5)
RsaI          557  GTAC                 GT^AC
Sau96I        495  GGCCC                G^G[ACGT]CC
ScaI          556  AGTACT               AGT^ACT
SfaNI         314  GCATC                GCATC(5/9)
SfuI          442  TTCGAA               TT^CGAA
SmaI          497  CCCGGG               CCC^GGG
SpeI          448  ACTAGT               A^CTAGT
SspI          292  AATATT               AAT^ATT
StyI          242  CCATGG               C^C[AT][AT]GG
TfiI          431  GAATC                G^A[AT]TC
TseI           33  GCTGC                G^C[AT]GC
Tsp509I       126  AATT                 ^AATT
TspRI         144  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          461  CCAGTATGAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          419  CTCGAG               C^TCGAG
XhoII         137  AGATCC               [GA]^GATC[CT]
XmaCI         497  CCCGGG               C^CCGGG
XmaI          497  CCCGGG               C^CCGGG
XmnI          279  GAAATCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          141  CCGC                 CCGC(-3/-1)
              204  GCGG                
BalI            2  TGGCCA               TGG^CCA
              195  TGGCCA              
BcnI          497  CCCGG                CC^[GC]GG
              498  CCGGG               
BfaI          256  CTAG                 C^TAG
              449  CTAG                
BstNI         492  CCTGG                CC^[AT]GG
              603  CCTGG               
Cac8I         193  GCTGGC               GC[ACGT]^[ACGT]GC
              402  GCAGGC              
DsaI          234  CCGTGG               C^C[GA][CT]GG
              242  CCATGG              
EaeI            2  TGGCCA               [CT]^GGCC[GA]
              195  TGGCCA              
EcoRII        492  CCTGG                ^CC[AT]GG
              603  CCTGG               
HapII         498  CCGG                 C^CGG
              525  CCGG                
HinfI          46  GACTC                G^A[ACGT]TC
              431  GAATC               
HpaII         498  CCGG                 C^CGG
              525  CCGG                
MaeI          256  CTAG                 C^TAG
              449  CTAG                
MaeIII        368  GTGAC                ^GT[ACGT]AC
              575  GTGAC               
MluNI           2  TGGCCA               TGG^CCA
              195  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              195  TGGCCA              
MspI          498  CCGG                 C^CGG
              525  CCGG                
MvaI          492  CCTGG                CC^[AT]GG
              603  CCTGG               
NciI          497  CCCGG                CC^[GC]GG
              498  CCGGG               
SmlI          419  CTCGAG               C^T[CT][GA]AG
              511  CTCAAG              
Tsp45I        368  GTGAC                ^GT[GC]AC
              575  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AvaI        BsaJI       BsiYI       BslI        BsmFI       
BsoBI       BsrI        BssKI       BstF5I      DpnII       
FokI        HaeIII      MboI        MboII       MnlI        
MwoI        NdeII       NlaIII      Sau3AI      ScrFI       
TaqI        TthHB8I     
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