Restriction map for pF1KB8199
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AvaII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmFI       
BsmI        Bsp1286I    Bsp1407I    BspDI       BspEI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HapII       
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MaeII       
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp45I      Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         103  CGCG                 CG^CG
AcsI          585  GAATTT               [GA]^AATT[CT]
AlwI          345  GATCC                GGATC(4/5)
ApoI          585  GAATTT               [GA]^AATT[CT]
Asp700I       387  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           81  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         185  TGCGCA               TGC^GCA
BfaI          479  CTAG                 C^TAG
BglI           65  GCCCTGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         573  AGATCT               A^GATCT
BpmI          545  CTGGAG               CTGGAG(16/14)
BsmBI         415  GAGACG               CGTCTC(1/5)
BspHI         146  TCATGA               T^CATGA
BsrBI          20  CCGCTC               CCGCTC(-3/-3)
BstAPI        532  GCACCACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         103  CGCG                 CG^CG
BstYI         573  AGATCT               [GA]^GATC[CT]
EaeI          494  TGGCCG               [CT]^GGCC[GA]
EarI          310  GAAGAG               CTCTTC(1/4)
EcoT14I       333  CCAAGG               C^C[AT][AT]GG
FauI           19  CCCGC                CCCGC(4/6)
FspI          185  TGCGCA               TGC^GCA
HgaI           81  GACGC                GACGC(5/10)
HinfI          31  GACTC                G^A[ACGT]TC
Ksp632I       310  GAAGAG               CTCTTC(1/4)
MaeI          479  CTAG                 C^TAG
MflI          573  AGATCT               [GA]^GATC[CT]
MspA1I        424  CAGCTG               C[AC]G^C[GT]G
MvnI          103  CGCG                 CG^CG
PflFI          81  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           31  GACTC                GAGTC(4/5)
PstI          172  CTGCAG               CTGCA^G
PvuII         424  CAGCTG               CAG^CTG
RcaI          146  TCATGA               T^CATGA
SfcI          172  CTGCAG               C^T[GA][CT]AG
SpeI          478  ACTAGT               A^CTAGT
StyI          333  CCAAGG               C^C[AT][AT]GG
TaqI           48  TCGA                 T^CGA
Tsp509I       586  AATT                 ^AATT
TspRI         341  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        81  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        48  TCGA                 T^CGA
XcmI          509  CCAAACAATCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         573  AGATCT               [GA]^GATC[CT]
XmnI          387  GAACACTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AvaI           35  CTCGGG               C^[CT]CG[GA]G
              113  CCCGAG              
BsoBI          35  CTCGGG               C^[CT]CG[GA]G
              113  CCCGAG              
BsrI          492  ACTGG                ACTGG(1/-1)
              544  ACTGG               
BstF5I        522  GGATG                GGATG(2/0)
              552  GGATG               
Cac8I         174  GCAGGC               GC[ACGT]^[ACGT]GC
              208  GCTCGC              
CfoI           14  GCGC                 GCG^C
              186  GCGC                
DpnII         345  GATC                 ^GATC
              574  GATC                
FokI          522  GGATG                GGATG(9/13)
              552  GGATG               
HaeIII        406  GGCC                 GG^CC
              495  GGCC                
HhaI           14  GCGC                 GCG^C
              186  GCGC                
HinP1I         14  GCGC                 G^CGC
              186  GCGC                
HphI          315  GGTGA                GGTGA(8/7)
              395  TCACC               
MboI          345  GATC                 ^GATC
              574  GATC                
MboII         219  GAAGA                GAAGA(8/7)
              310  GAAGA               
NdeII         345  GATC                 ^GATC
              574  GATC                
NlaIII        147  CATG                 CATG^
              409  CATG                
NlaIV         109  GGAACC               GG[ACGT]^[ACGT]CC
              123  GGAGCC              
Sau3AI        345  GATC                 ^GATC
              574  GATC                
SfaNI         373  GCATC                GCATC(5/9)
              446  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AciI        AluI        BbvI        BsaJI       BsiYI       
BslI        BsmAI       BssKI       BstNI       DdeI        
EcoRII      Fnu4HI      ItaI        MnlI        MseI        
MvaI        MwoI        ScrFI       Tru9I       TseI        
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