Restriction map for pF1KB8197
##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HapII       Hin1I       HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        TfiI        Tru9I       TseI        
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


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# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI           34  GTCGAC               GT^[AC][GT]AC
AccII          55  CGCG                 CG^CG
AciI           54  CCGC                 CCGC(-3/-1)
AluI           68  AGCT                 AG^CT
Aor51HI       428  AGCGCT               AGC^GCT
Asp700I       100  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI           79  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII          43  GAGCCC               G[GA]GC[CT]^C
BglI          206  GCCATAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          122  CTGGAG               CTGGAG(16/14)
BsgI          342  CTGCAC               GTGCAG(16/14)
BsiYI         230  CCATCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          230  CCATCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          58  GTCTC                GTCTC(1/5)
BsmBI          57  CGTCTC               CGTCTC(1/5)
BsmFI          85  GTCCC                GGGAC(10/14)
BstUI          55  CGCG                 CG^CG
CfoI          429  GCGC                 GCG^C
DpnII         465  GATC                 ^GATC
DrdI           25  GACATTGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      428  AGCGCT               AGC^GCT
Fnu4HI         53  GCCGC                GC^[ACGT]GC
HaeII         428  AGCGCT               [GA]GCGC^[CT]
HgaI           56  GCGTC                GACGC(5/10)
HhaI          429  GCGC                 GCG^C
HinP1I        429  GCGC                 G^CGC
ItaI           53  GCCGC                GC^[ACGT]GC
MaeIII        194  GTGAC                ^GT[ACGT]AC
MboI          465  GATC                 ^GATC
MnlI          418  GAGG                 CCTC(7/6)
MvnI           55  CGCG                 CG^CG
NdeII         465  GATC                 ^GATC
NspI          375  GCATGT               [GA]CATG^[CT]
PflFI          79  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SalI           34  GTCGAC               G^TCGAC
Sau3AI        465  GATC                 ^GATC
SfaNI         287  GCATC                GCATC(5/9)
SspI          409  AATATT               AAT^ATT
Tsp45I        194  GTGAC                ^GT[GC]AC
Tth111I        79  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          207  CCATAATGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          100  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          102  AAATTT               [GA]^AATT[CT]
              244  AAATTT              
ApoI          102  AAATTT               [GA]^AATT[CT]
              244  AAATTT              
AspHI         110  GTGCTC               G[AT]GC[AT]^C
              117  GAGCAC              
BsiHKAI       110  GTGCTC               G[AT]GC[AT]^C
              117  GAGCAC              
BsrDI          27  CATTGC               GCAATG(2/0)
              471  CATTGC              
BstF5I        264  CATCC                GGATG(2/0)
              366  GGATG               
Cac8I          41  GCGAGC               GC[ACGT]^[ACGT]GC
              371  GCAAGC              
DdeI          115  CTGAG                C^T[ACGT]AG
              356  CTGAG               
DraII          83  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              279  GGGTCCT             
DsaI          296  CCATGG               C^C[GA][CT]GG
              422  CCATGG              
EcoO109I       83  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              279  GGGTCCT             
FokI          264  CATCC                GGATG(9/13)
              366  GGATG               
HaeIII         52  GGCC                 GG^CC
              420  GGCC                
HincII          4  GTCAAC               GT[CT]^[GA]AC
               34  GTCGAC              
HindII          4  GTCAAC               GT[CT]^[GA]AC
               34  GTCGAC              
HphI          193  GGTGA                GGTGA(8/7)
              467  TCACC               
NcoI          296  CCATGG               C^CATGG
              422  CCATGG              
PpuMI          83  AGGTCCC              [GA]G^G[AT]CC[CT]
              279  GGGTCCT             


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AvaII       BmyI        BsaJI       Bsp1286I    BsrI        
BssKI       BstNI       Cfr13I      EcoRII      EcoT14I     
MboII       MvaI        MwoI        NlaIII      NlaIV       
Sau96I      ScrFI       StyI        TaqI        Tsp509I     
TspRI       TthHB8I     
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