Restriction map for pF1KB8196
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AviII       
AvrII       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaBI       BsiHKAI     BsiWI       BsmBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrGI       BssSI       Bst1107I    BstAPI      BstZ17I     
ClaI        CpoI        DraI        DraIII      DrdI        
EarI        Eco47III    EcoNI       EcoRV       EcoT22I     
FbaI        FseI        FspI        KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccIII       1054  TCCGGA               T^CCGGA
AcsI         1044  GAATTC               [GA]^AATT[CT]
AflIII        641  ACACGT               A^C[GA][CT]GT
AocI         1360  CCTGAGG              CC^T[ACGT]AGG
ApoI         1044  GAATTC               [GA]^AATT[CT]
BalI         1241  TGGCCA               TGG^CCA
BamHI         669  GGATCC               G^GATCC
BanI          506  GGCACC               G^G[CT][GA]CC
BbvCI         693  GCTGAGG              CCTCAGC(-5/-2)
BfaI          407  CTAG                 C^TAG
BsaAI         642  CACGTA               [CT]AC^GT[GA]
BsaWI        1054  TCCGGA               [AT]^CCGG[AT]
BseAI        1054  TCCGGA               T^CCGGA
BsiEI         386  CGGCCG               CG[GA][CT]^CG
BsmI           65  GCATTC               GAATGC(1/-1)
BspEI        1054  TCCGGA               T^CCGGA
BspMI         474  ACCTGC               ACCTGC(4/8)
BsrFI         503  ACCGGC               [GA]^CCGG[CT]
BssHII        309  GCGCGC               G^CGCGC
BstBI        1292  TTCGAA               TT^CGAA
BstEII        255  GGTGACC              G^GT[ACGT]ACC
BstPI         255  GGTGACC              G^GT[ACGT]ACC
BstXI         845  CCAGAGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1360  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        503  ACCGGC               [GA]^CCGG[CT]
EagI          386  CGGCCG               C^GGCCG
EclXI         386  CGGCCG               C^GGCCG
Eco52I        386  CGGCCG               C^GGCCG
Eco81I       1360  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       255  GGTGACC              G^GT[ACGT]ACC
EcoRI        1044  GAATTC               G^AATTC
EcoT14I       776  CCATGG               C^C[AT][AT]GG
HincII        931  GTTAAC               GT[CT]^[GA]AC
HindII        931  GTTAAC               GT[CT]^[GA]AC
HindIII      1146  AAGCTT               A^AGCTT
HpaI          931  GTTAAC               GTT^AAC
MaeI          407  CTAG                 C^TAG
MluNI        1241  TGGCCA               TGG^CCA
MroI         1054  TCCGGA               T^CCGGA
MscI         1241  TGGCCA               TGG^CCA
MseI          932  TTAA                 T^TAA
NcoI          776  CCATGG               C^CATGG
NspI          208  GCATGC               [GA]CATG^[CT]
NspV         1292  TTCGAA               TT^CGAA
PstI          796  CTGCAG               CTGCA^G
SfuI         1292  TTCGAA               TT^CGAA
SphI          208  GCATGC               GCATG^C
StuI          281  AGGCCT               AGG^CCT
StyI          776  CCATGG               C^C[AT][AT]GG
Tru9I         932  TTAA                 T^TAA
Tsp509I      1045  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AatII         117  GACGTC               GACGT^C
              216  GACGTC              
AcyI          117  GACGTC               G[GA]^CG[CT]C
              216  GACGTC              
AlwNI         165  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              470  CAGCACCTG           
BbsI          465  GAAGAC               GAAGAC(2/6)
             1010  GTCTTC              
BpmI           33  CTGGAG               CTGGAG(16/14)
              917  CTGGAG              
BpuAI         465  GAAGAC               GAAGAC(2/6)
             1010  GTCTTC              
BsaHI         117  GACGTC               G[GA]^CG[CT]C
              216  GACGTC              
BsaI           81  GAGACC               GGTCTC(1/5)
              848  GAGACC              
BseRI        1050  CTCCTC               GAGGAG(10/8)
               78  GAGGAG              
BsrI          164  CCAGT                ACTGG(1/-1)
             1305  CCAGT               
EaeI          386  CGGCCG               [CT]^GGCC[GA]
             1241  TGGCCA              
HgaI          737  GACGC                GACGC(5/10)
              837  GCGTC               
Hin1I         117  GACGTC               G[GA]^CG[CT]C
              216  GACGTC              
MslI          660  CACAGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1083  CATTGGGGTG          
PflMI         164  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              591  CCATGTGGTGG         
SfcI          796  CTGCAG               C^T[GA][CT]AG
             1129  CTACAG              
Van91I        164  CCAGTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              591  CCATGTGGTGG         
XcmI          584  CCAACGCCCATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              722  CCACGCTGCCTCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   87 enzymes) #
#########################################

AccII       AciI        AfaI        AhdI        AluI        
AlwI        ApaI        Asp700I     AspEI       AvaI        
AvaII       BanII       BbvI        BcnI        BlpI        
BmyI        Bpu1102I    BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        Eam1105I    EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmaI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     XhoII       XmaCI       
XmaI        XmnI        
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