Restriction map for pF1KB8196
 (Linear) (Six-base) MAPSORT of: KIBB8196.seq  Check: 4248  from: 1  to: 1374



KIBB8196.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     483    1374 
   Size:        483     891
 
AatII G_ACGT'C

Cuts at:      0     121     220    1374 
   Size:        121      99    1154

  Fragments arranged by size: 

                1154     121      99
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1292    1374 
   Size:       1292      82
 
AflIII A'CryG_T

Cuts at:      0     641    1374 
   Size:        641     733
 
AhdI GACnn_n'nnGTC

Cuts at:      0     164     230    1106    1374 
   Size:        164      66     876     268

  Fragments arranged by size: 

                 876     268     164      66
 
AleI CACnn'nnGTG

Cuts at:      0     664    1374 
   Size:        664     710
 
AlwNI CAG_nnn'CTG

Cuts at:      0     170     475    1374 
   Size:        170     305     899

  Fragments arranged by size: 

                 899     305     170
 
ApaI G_GGCC'C

Cuts at:      0     111     144     177    1374 
   Size:        111      33      33    1197

  Fragments arranged by size: 

                1197     111      33      33
 
ApoI r'AATT_y

Cuts at:      0    1044    1374 
   Size:       1044     330
 
AvaI C'yCGr_G

Cuts at:      0     137     143     203     228     236     275    1374 
   Size:        137       6      60      25       8      39    1099

  Fragments arranged by size: 

                1099     137      60      39      25       8       6
 
BamHI G'GATC_C

Cuts at:      0     669    1374 
   Size:        669     705
 
BanI G'GyrC_C

Cuts at:      0     506    1374 
   Size:        506     868
 
BanII G_rGCy'C

Cuts at:      0     111     144     177     575    1198    1374 
   Size:        111      33      33     398     623     176

  Fragments arranged by size: 

                 623     398     176     111      33      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     472    1003    1374 
   Size:        472     531     371

  Fragments arranged by size: 

                 531     472     371
 
BbvCI CC'TCA_GC

Cuts at:      0     694    1374 
   Size:        694     680
 
BlpI GC'TnA_GC

Cuts at:      0     631     741     833    1374 
   Size:        631     110      92     541

  Fragments arranged by size: 

                 631     541     110      92
 
Bme1580I G_kGCm'C

Cuts at:      0     111     144     177     243    1374 
   Size:        111      33      33      66    1131

  Fragments arranged by size: 

                1131     111      66      33      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     158    1299    1374 
   Size:        158    1141      75

  Fragments arranged by size: 

                1141     158      75
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     938    1374 
   Size:         54     884     436

  Fragments arranged by size: 

                 884     436      54
 
Bpu10I CC'TnA_GC

Cuts at:      0     546     694    1374 
   Size:        546     148     680

  Fragments arranged by size: 

                 680     546     148
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75     842    1374 
   Size:         75     767     532

  Fragments arranged by size: 

                 767     532      75
 
BsaAI yAC'GTr

Cuts at:      0     644    1374 
   Size:        644     730
 
BsaHI Gr'CG_yC

Cuts at:      0     118     217    1374 
   Size:        118      99    1157

  Fragments arranged by size: 

                1157     118      99
 
BsaWI w'CCGG_w

Cuts at:      0    1054    1374 
   Size:       1054     320
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      72     102    1374 
   Size:         72      30    1272

  Fragments arranged by size: 

                1272      72      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      93    1041    1374 
   Size:         93     948     333

  Fragments arranged by size: 

                 948     333      93
 
BseYI C'CCAG_C

Cuts at:      0      71     330     337    1070    1111    1374 
   Size:         71     259       7     733      41     263

  Fragments arranged by size: 

                 733     263     259      71      41       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47    1187    1336    1374 
   Size:         47    1140     149      38

  Fragments arranged by size: 

                1140     149      47      38
 
BsiEI CG_ry'CG

Cuts at:      0     389    1374 
   Size:        389     985
 
BsmI GAATG_Cn'

Cuts at:      0      65    1374 
   Size:         65    1309
 
Bsp1286I G_dGCh'C

Cuts at:      0     111     144     177     243     575    1198    1374 
   Size:        111      33      33      66     332     623     176

  Fragments arranged by size: 

                 623     332     176     111      66      33      33
 
BspEI T'CCGG_A

Cuts at:      0    1054    1374 
   Size:       1054     320
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     483    1374 
   Size:        483     891
 
BsrFI r'CCGG_y

Cuts at:      0     503    1374 
   Size:        503     871
 
BssHII G'CGCG_C

Cuts at:      0     309    1374 
   Size:        309    1065
 
BstBI TT'CG_AA

Cuts at:      0    1293    1374 
   Size:       1293      81
 
BstEII G'GTnAC_C

Cuts at:      0     255    1374 
   Size:        255    1119
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     852    1374 
   Size:        852     522
 
BstYI r'GATC_y

Cuts at:      0     494     669    1319    1374 
   Size:        494     175     650      55

  Fragments arranged by size: 

                 650     494     175      55
 
Bsu36I CC'TnA_GG

Cuts at:      0    1361    1374 
   Size:       1361      13
 
BtgI C'CryG_G

Cuts at:      0     479     653     674     776    1374 
   Size:        479     174      21     102     598

  Fragments arranged by size: 

                 598     479     174     102      21
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     432     669    1374 
   Size:        432     237     705

  Fragments arranged by size: 

                 705     432     237
 
BtsI GCAGTG_nn'

Cuts at:      0     353     793    1319    1374 
   Size:        353     440     526      55

  Fragments arranged by size: 

                 526     440     353      55
 
EaeI y'GGCC_r

Cuts at:      0     386    1241    1374 
   Size:        386     855     133

  Fragments arranged by size: 

                 855     386     133
 
EagI C'GGCC_G

Cuts at:      0     386    1374 
   Size:        386     988
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      54     938    1292    1374 
   Size:         54     884     354      82

  Fragments arranged by size: 

                 884     354      82      54
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108     158     224     232     272     379     528     922 
   Size:        108      50      66       8      40     107     149     394

Cuts at:    922    1067    1374 
   Size:        145     307

  Fragments arranged by size: 

                 394     307     149     145     108     107      66      50
                  40       8
 
EcoRI G'AATT_C

Cuts at:      0    1044    1374 
   Size:       1044     330
 
HaeII r_GCGC'y

Cuts at:      0     331     338    1080    1374 
   Size:        331       7     742     294

  Fragments arranged by size: 

                 742     331     294       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     150     182     216     248    1092    1124    1374 
   Size:        150      32      34      32     844      32     250

  Fragments arranged by size: 

                 844     250     150      34      32      32      32
 
HincII GTy'rAC

Cuts at:      0     933    1374 
   Size:        933     441
 
HindIII A'AGCT_T

Cuts at:      0    1146    1374 
   Size:       1146     228
 
HpaI GTT'AAC

Cuts at:      0     933    1374 
   Size:        933     441
 
MscI TGG'CCA

Cuts at:      0    1243    1374 
   Size:       1243     131
 
MslI CAynn'nnrTG

Cuts at:      0     664    1087    1374 
   Size:        664     423     287

  Fragments arranged by size: 

                 664     423     287
 
MspA1I CmG'CkG

Cuts at:      0     664     745     979    1074    1374 
   Size:        664      81     234      95     300

  Fragments arranged by size: 

                 664     300     234      95      81
 
NcoI C'CATG_G

Cuts at:      0     776    1374 
   Size:        776     598
 
NspI r_CATG'y

Cuts at:      0     212    1374 
   Size:        212    1162
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     170     597    1374 
   Size:        170     427     777

  Fragments arranged by size: 

                 777     427     170
 
PfoI T'CCnGG_A

Cuts at:      0     497    1374 
   Size:        497     877
 
PpuMI rG'GwC_Cy

Cuts at:      0     158     224     232     272     379     528     922    1067 
   Size:        158      66       8      40     107     149     394     145

Cuts at:   1067    1374 
   Size:        307

  Fragments arranged by size: 

                 394     307     158     149     145     107      66      40
                   8
 
PspOMI G'GGCC_C

Cuts at:      0     107     140     173    1374 
   Size:        107      33      33    1201

  Fragments arranged by size: 

                1201     107      33      33
 
PstI C_TGCA'G

Cuts at:      0     800    1374 
   Size:        800     574
 
PvuII CAG'CTG

Cuts at:      0     664     745     979    1374 
   Size:        664      81     234     395

  Fragments arranged by size: 

                 664     395     234      81
 
SanDI GG'GwC_CC

Cuts at:      0     158     224     232    1067    1374 
   Size:        158      66       8     835     307

  Fragments arranged by size: 

                 835     307     158      66       8
 
SfcI C'TryA_G

Cuts at:      0     796    1129    1374 
   Size:        796     333     245

  Fragments arranged by size: 

                 796     333     245
 
SmaI CCC'GGG

Cuts at:      0     139     230     238     277    1374 
   Size:        139      91       8      39    1097

  Fragments arranged by size: 

                1097     139      91      39       8
 
SphI G_CATG'C

Cuts at:      0     212    1374 
   Size:        212    1162
 
StuI AGG'CCT

Cuts at:      0     283    1374 
   Size:        283    1091
 
StyI C'CwwG_G

Cuts at:      0     776    1374 
   Size:        776     598
 
TatI w'GTAC_w

Cuts at:      0     437    1170    1374 
   Size:        437     733     204

  Fragments arranged by size: 

                 733     437     204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     542     574    1374 
   Size:        542      32     800

  Fragments arranged by size: 

                 800     542      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     591     729    1374 
   Size:        591     138     645

  Fragments arranged by size: 

                 645     591     138
 
XmaI C'CCGG_G

Cuts at:      0     137     228     236     275    1374 
   Size:        137      91       8      39    1099

  Fragments arranged by size: 

                1099     137      91      39       8
 
XmnI GAAnn'nnTTC

Cuts at:      0     303     881    1289    1374 
   Size:        303     578     408      85

  Fragments arranged by size: 

                 578     408     303      85
 
ZraI GAC'GTC

Cuts at:      0     119     218    1374 
   Size:        119      99    1156

  Fragments arranged by size: 

                1156     119      99
 

 Enzymes that do cut:

     AarI    AatII     AcuI   AflIII     AhdI     AleI    AlwNI     ApaI
     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI     BsmI Bsp1286I    BspEI
    BspMI    BsrFI   BssHII    BstBI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     EaeI     EagI  Eco57MI EcoO109I    EcoRI
    HaeII    Hin4I   HincII  HindIII     HpaI     MscI     MslI   MspA1I
     NcoI     NspI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII
    SanDI     SfcI     SmaI     SphI     StuI     StyI     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI
    BpuEI    BsaBI  BsiHKAI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI
    BsrGI    BssSI   BstAPI  BstZ17I     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI     XhoI
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