Restriction map for pF1KB8186
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaBI       BsaHI       BsaI        
BseAI       BsgI        BsiEI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HpaI        KasI        
KpnI        Ksp632I     MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NciI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        TaqI        Tsp45I      Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII           7  CGCG                 CG^CG
AgeI           82  ACCGGT               A^CCGGT
Alw44I        636  GTGCAC               G^TGCAC
AlwNI         766  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         636  GTGCAC               G^TGCAC
Asp700I        36  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         636  GTGCAC               G[AT]GC[AT]^C
BbsI          252  GAAGAC               GAAGAC(2/6)
BfaI          550  CTAG                 C^TAG
BglI          134  GCCTGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         252  GAAGAC               GAAGAC(2/6)
BsaAI         115  TACGTA               [CT]AC^GT[GA]
BsaWI          82  ACCGGT               [AT]^CCGG[AT]
BsiHKAI       636  GTGCAC               G[AT]GC[AT]^C
BsiWI         833  CGTACG               C^GTACG
BsmFI         876  GGGAC                GGGAC(10/14)
BspHI         420  TCATGA               T^CATGA
BspMI         761  ACCTGC               ACCTGC(4/8)
BsrFI          82  ACCGGT               [GA]^CCGG[CT]
BstUI           7  CGCG                 CG^CG
Cfr10I         82  ACCGGT               [GA]^CCGG[CT]
EaeI          776  TGGCCG               [CT]^GGCC[GA]
HapII          83  CCGG                 C^CGG
HindIII       454  AAGCTT               A^AGCTT
HpaII          83  CCGG                 C^CGG
KspI            6  CCGCGG               CCGC^GG
MaeI          550  CTAG                 C^TAG
MslI          159  CATCCAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           83  CCGG                 C^CGG
MvnI            7  CGCG                 CG^CG
NcoI          800  CCATGG               C^CATGG
NdeI          299  CATATG               CA^TATG
PinAI          82  ACCGGT               A^CCGGT
PleI          580  GAGTC                GAGTC(4/5)
PvuII          30  CAGCTG               CAG^CTG
RcaI          420  TCATGA               T^CATGA
SacII           6  CCGCGG               CCGC^GG
SnaBI         115  TACGTA               TAC^GTA
SspI          120  AATATT               AAT^ATT
XcmI          791  CCAATCTTACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI           36  GAAGAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaII          80  GGACC                G^G[AT]CC
              207  GGACC               
BcgI          436  CGAAAGGTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              143  GCATCTTCATCG        
BmyI          385  GTGCCC               G[AGT]GC[ACT]^C
              636  GTGCAC              
BsiYI         730  CCGTACTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              867  CCGTTTTCAGG         
BslI          730  CCGTACTATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              867  CCGTTTTCAGG         
BsmAI         410  GAGAC                GTCTC(1/5)
              589  GAGAC               
Bsp1286I      385  GTGCCC               G[AGT]GC[ACT]^C
              636  GTGCAC              
BstAPI        515  GCAAACCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              765  GCAGCCCCTGC         
BstF5I        159  CATCC                GGATG(2/0)
              354  GGATG               
BstYI         101  AGATCC               [GA]^GATC[CT]
              245  AGATCC              
DsaI            6  CCGCGG               C^C[GA][CT]GG
              800  CCATGG              
FokI          159  CATCC                GGATG(9/13)
              354  GGATG               
MaeII         116  ACGT                 A^CGT
              879  ACGT                
MflI          101  AGATCC               [GA]^GATC[CT]
              245  AGATCC              
MspA1I          6  CCGCGG               C[AC]G^C[GT]G
               30  CAGCTG              
SfaNI         168  GATGC                GCATC(5/9)
              143  GCATC               
XhoII         101  AGATCC               [GA]^GATC[CT]
              245  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BsaJI       BseRI       BsrI        
BssKI       BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HinfI       HphI        ItaI        MboI        
MboII       MnlI        MseI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
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