Restriction map for pF1KB8185
 (Linear) (Six-base) MAPSORT of: KIBB8185.seq  Check: 4187  from: 1  to: 1383



KIBB8185.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655    1150    1383 
   Size:        655     495     233

  Fragments arranged by size: 

                 655     495     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     789    1383 
   Size:        789     594
 
BanI G'GyrC_C

Cuts at:      0     560     733    1383 
   Size:        560     173     650

  Fragments arranged by size: 

                 650     560     173
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     661     938    1383 
   Size:        661     277     445

  Fragments arranged by size: 

                 661     445     277
 
BglI GCCn_nnn'nGGC

Cuts at:      0     376    1383 
   Size:        376    1007
 
Bme1580I G_kGCm'C

Cuts at:      0     326     427     736    1383 
   Size:        326     101     309     647

  Fragments arranged by size: 

                 647     326     309     101
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     302     692    1132    1383 
   Size:        254      48     390     440     251

  Fragments arranged by size: 

                 440     390     254     251      48
 
Bpu10I CC'TnA_GC

Cuts at:      0      94    1383 
   Size:         94    1289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     553     628     646    1294    1383 
   Size:        553      75      18     648      89

  Fragments arranged by size: 

                 648     553      89      75      18
 
BsaBI GATnn'nnATC

Cuts at:      0    1162    1383 
   Size:       1162     221
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     948     978    1383 
   Size:        948      30     405

  Fragments arranged by size: 

                 948     405      30
 
BseYI C'CCAG_C

Cuts at:      0     325     389     552    1383 
   Size:        325      64     163     831

  Fragments arranged by size: 

                 831     325     163      64
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1327    1383 
   Size:       1327      56
 
BsiEI CG_ry'CG

Cuts at:      0     654    1383 
   Size:        654     729
 
Bsp1286I G_dGCh'C

Cuts at:      0     326     427     736    1383 
   Size:        326     101     309     647

  Fragments arranged by size: 

                 647     326     309     101
 
BspHI T'CATG_A

Cuts at:      0     863    1383 
   Size:        863     520
 
BsrBI CCG'CTC

Cuts at:      0     183    1142    1299    1383 
   Size:        183     959     157      84

  Fragments arranged by size: 

                 959     183     157      84
 
BsrDI GCAATG_nn'

Cuts at:      0     610     720    1383 
   Size:        610     110     663

  Fragments arranged by size: 

                 663     610     110
 
BsrFI r'CCGG_y

Cuts at:      0     557    1383 
   Size:        557     826
 
BstYI r'GATC_y

Cuts at:      0      89     200    1115    1383 
   Size:         89     111     915     268

  Fragments arranged by size: 

                 915     268     111      89
 
BtgI C'CryG_G

Cuts at:      0     574    1383 
   Size:        574     809
 
BtsI GCAGTG_nn'

Cuts at:      0     386    1383 
   Size:        386     997
 
DraIII CAC_nnn'GTG

Cuts at:      0     897    1383 
   Size:        897     486
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     517    1383 
   Size:        517     866
 
EaeI y'GGCC_r

Cuts at:      0      83     114     651     800    1290    1383 
   Size:         83      31     537     149     490      93

  Fragments arranged by size: 

                 537     490     149      93      83      31
 
EagI C'GGCC_G

Cuts at:      0     651    1383 
   Size:        651     732
 
EarI CTCTTCn'nnn_

Cuts at:      0     859    1383 
   Size:        859     524
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19      66    1383 
   Size:         19      47    1317

  Fragments arranged by size: 

                1317      47      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     302     655     692    1132    1150    1383 
   Size:        254      48     353      37     440      18     233

  Fragments arranged by size: 

                 440     353     254     233      48      37      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0     254    1043    1383 
   Size:        254     789     340

  Fragments arranged by size: 

                 789     340     254
 
HaeII r_GCGC'y

Cuts at:      0     978    1383 
   Size:        978     405
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453     907     939    1195    1227    1383 
   Size:        421      32     454      32     256      32     156

  Fragments arranged by size: 

                 454     421     256     156      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     121    1383 
   Size:        121    1262
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1115    1383 
   Size:       1115     268
 
MscI TGG'CCA

Cuts at:      0     802    1292    1383 
   Size:        802     490      91

  Fragments arranged by size: 

                 802     490      91
 
MslI CAynn'nnrTG

Cuts at:      0     526    1383 
   Size:        526     857
 
NaeI GCC'GGC

Cuts at:      0     559    1383 
   Size:        559     824
 
NgoMIV G'CCGG_C

Cuts at:      0     557    1383 
   Size:        557     826
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     897    1361    1383 
   Size:        897     464      22

  Fragments arranged by size: 

                 897     464      22
 
PfoI T'CCnGG_A

Cuts at:      0    1109    1383 
   Size:       1109     274
 
PpuMI rG'GwC_Cy

Cuts at:      0     254    1043    1383 
   Size:        254     789     340

  Fragments arranged by size: 

                 789     340     254
 
PstI C_TGCA'G

Cuts at:      0    1223    1383 
   Size:       1223     160
 
SanDI GG'GwC_CC

Cuts at:      0     254    1043    1383 
   Size:        254     789     340

  Fragments arranged by size: 

                 789     340     254
 
SfcI C'TryA_G

Cuts at:      0    1219    1383 
   Size:       1219     164
 
SmlI C'TyrA_G

Cuts at:      0     568     643     661    1309    1383 
   Size:        568      75      18     648      74

  Fragments arranged by size: 

                 648     568      75      74      18
 
StyI C'CwwG_G

Cuts at:      0     911    1068    1355    1383 
   Size:        911     157     287      28

  Fragments arranged by size: 

                 911     287     157      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     159    1383 
   Size:        159    1224
 
TatI w'GTAC_w

Cuts at:      0     830    1256    1383 
   Size:        830     426     127

  Fragments arranged by size: 

                 830     426     127
 
Tth111I GACn'n_nGTC

Cuts at:      0     517    1383 
   Size:        517     866
 
XmnI GAAnn'nnTTC

Cuts at:      0     124    1383 
   Size:        124    1259
 

 Enzymes that do cut:

     AcuI    AlwNI     BanI     BbsI     BglI Bme1580I     BpmI   Bpu10I
    BpuEI    BsaBI    BsaXI    BseYI     BsgI    BsiEI Bsp1286I    BspHI
    BsrBI    BsrDI    BsrFI    BstYI     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I
  HindIII     MmeI     MscI     MslI     NaeI   NgoMIV    PflMI     PfoI
    PpuMI     PstI    SanDI     SfcI     SmlI     StyI    TaqII     TatI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI
    BseRI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII     HpaI     KasI     KpnI
     MfeI     MluI   MspA1I     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     ZraI
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