Restriction map for pF1KB8169
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BbeI        BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmI        BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HapII       HhaI        HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MspI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmeI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacII       SalI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI          853  GTCTAC               GT^[AC][GT]AC
AccII         764  CGCG                 CG^CG
AcsI           25  AAATTT               [GA]^AATT[CT]
AcyI          361  GACGCC               G[GA]^CG[CT]C
AhdI          583  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         112  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           25  AAATTT               [GA]^AATT[CT]
AspEI         583  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          486  CCCGAG               C^[CT]CG[GA]G
BalI          879  TGGCCA               TGG^CCA
BanI          941  GGTGCC               G^G[CT][GA]CC
BbrPI         787  CACGTG               CAC^GTG
BbsI          285  GAAGAC               GAAGAC(2/6)
BlpI          637  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      637  GCTCAGC              GC^T[ACGT]AGC
BpuAI         285  GAAGAC               GAAGAC(2/6)
BsaAI         787  CACGTG               [CT]AC^GT[GA]
BsaHI         361  GACGCC               G[GA]^CG[CT]C
BsaI           39  GGTCTC               GGTCTC(1/5)
BseRI         970  GAGGAG               GAGGAG(10/8)
BsmBI         359  GAGACG               CGTCTC(1/5)
BsoBI         486  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      713  TGTACA               T^GTACA
BspHI         890  TCATGA               T^CATGA
BsrGI         713  TGTACA               T^GTACA
BstAPI        370  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         764  CGCG                 CG^CG
BstXI         135  CCAGGTCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         637  GCTCAGC              GC^T[ACGT]AGC
DrdI          130  GACACCCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          141  CCATGG               C^C[GA][CT]GG
EaeI          879  TGGCCA               [CT]^GGCC[GA]
Eam1105I      583  GACATCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          555  CTCTTC               CTCTTC(1/4)
Hin1I         361  GACGCC               G[GA]^CG[CT]C
HinfI         340  GATTC                G^A[ACGT]TC
Ksp632I       555  CTCTTC               CTCTTC(1/4)
MaeII         788  ACGT                 A^CGT
MluNI         879  TGGCCA               TGG^CCA
MscI          879  TGGCCA               TGG^CCA
MseI          698  TTAA                 T^TAA
MslI          502  CACTTTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        276  CAGCGG               C[AC]G^C[GT]G
MvnI          764  CGCG                 CG^CG
NcoI          141  CCATGG               C^CATGG
PmaCI         787  CACGTG               CAC^GTG
PmlI          787  CACGTG               CAC^GTG
RcaI          890  TCATGA               T^CATGA
SapI          554  GCTCTTC              GCTCTTC(1/4)
SbfI          367  CCTGCAGG             CCTGCA^GG
SfaNI         376  GATGC                GCATC(5/9)
Sse8387I      367  CCTGCAGG             CCTGCA^GG
SspBI         713  TGTACA               T^GTACA
TaqI          606  TCGA                 T^CGA
TfiI          340  GATTC                G^A[AT]TC
Tru9I         698  TTAA                 T^TAA
TthHB8I       606  TCGA                 T^CGA
XbaI          171  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AfaI          714  GTAC                 GT^AC
              803  GTAC                
ApaI          187  GGGCCC               GGGCC^C
              907  GGGCCC              
Asp700I       150  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              429  GAACGATTTC          
AspHI         316  GAGCTC               G[AT]GC[AT]^C
              552  GAGCTC              
BamHI         298  GGATCC               G^GATCC
              725  GGATCC              
BsgI          499  CTGCAC               GTGCAG(16/14)
              232  GTGCAG              
BsiHKAI       316  GAGCTC               G[AT]GC[AT]^C
              552  GAGCTC              
BspMI          60  GCAGGT               ACCTGC(4/8)
              261  GCAGGT              
BstYI         298  GGATCC               [GA]^GATC[CT]
              725  GGATCC              
DraIII        787  CACGTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              933  CACCTGGTG           
EcoT14I       141  CCATGG               C^C[AT][AT]GG
              828  CCAAGG              
HgaI          361  GACGC                GACGC(5/10)
              765  GCGTC               
MflI          298  GGATCC               [GA]^GATC[CT]
              725  GGATCC              
NlaIII        142  CATG                 CATG^
              891  CATG                
PpuMI         476  GGGACCC              [GA]G^G[AT]CC[CT]
              773  AGGTCCT             
RsaI          714  GTAC                 GT^AC
              803  GTAC                
SacI          316  GAGCTC               GAGCT^C
              552  GAGCTC              
SexAI         884  ACCAGGT              A^CC[AT]GGT
              934  ACCTGGT             
StyI          141  CCATGG               C^C[AT][AT]GG
              828  CCAAGG              
Tsp45I        568  GTCAC                ^GT[GC]AC
              581  GTGAC               
XhoII         298  GGATCC               [GA]^GATC[CT]
              725  GGATCC              
XmnI          150  GAAATTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              429  GAACGATTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   50 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BanII       
BbvI        BfaI        BglI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HphI        ItaI        MaeI        MaeIII      
MboI        MboII       MnlI        MvaI        MwoI        
NdeII       NlaIV       PstI        Sau3AI      Sau96I      
ScrFI       SfcI        TseI        Tsp509I     TspRI       
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