Restriction map for pF1KB8169
 (Circular) (Six-base) MAPSORT of: pF1KB8169.seq  Check: 6914  from: 1  to: 4109



pF1KB8169  4109 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    159     159 
   Size:       4109
 
AccI GT'mk_AC

Cuts at:    962    1147     962 
   Size:        185    3924
 
Acc65I G'GTAC_C

Cuts at:   1125    1125 
   Size:       4109
 
AclI AA'CG_TT

Cuts at:   3949    3949 
   Size:       4109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1934    2366    3113    3381    1934 
   Size:        432     747     268    2662

  Fragments arranged by size: 

                2662     747     432     268
 
AflIII A'CryG_T

Cuts at:   1461    2565    3542    1461 
   Size:       1104     977    2028

  Fragments arranged by size: 

                2028    1104     977
 
AgeI A'CCGG_T

Cuts at:   2497    2497 
   Size:       4109
 
AhdI GACnn_n'nnGTC

Cuts at:    696     696 
   Size:       4109
 
AleI CACnn'nnGTG

Cuts at:    614     614 
   Size:       4109
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    529     561     529 
   Size:         32    4077
 
AlwNI CAG_nnn'CTG

Cuts at:    225    1414    2981     225 
   Size:       1189    1567    1353

  Fragments arranged by size: 

                1567    1353    1189
 
ApaI G_GGCC'C

Cuts at:    299    1019     299 
   Size:        720    3389
 
ApaLI G'TGCA_C

Cuts at:   2879    2879 
   Size:       4109
 
ApoI r'AATT_y

Cuts at:    133    1113    3462    3718     133 
   Size:        980    2349     256     524

  Fragments arranged by size: 

                2349     980     524     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4109
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4109
 
AvaI C'yCGr_G

Cuts at:    594    1129     594 
   Size:        535    3574
 
BamHI G'GATC_C

Cuts at:    406     833    1134     406 
   Size:        427     301    3381

  Fragments arranged by size: 

                3381     427     301
 
BanI G'GyrC_C

Cuts at:   1049    1125    1539    1787    1822    1049 
   Size:         76     414     248      35    3336

  Fragments arranged by size: 

                3336     414     248      76      35
 
BanII G_rGCy'C

Cuts at:    299     383     428     664    1019    1123    2153     299 
   Size:         84      45     236     355     104    1030    2255

  Fragments arranged by size: 

                2255    1030     355     236     104      84      45
 
BbeI G_GCGC'C

Cuts at:   1543    1791    1543 
   Size:        248    3861
 
BbsI GAAGACnn'nnnn_

Cuts at:    400     400 
   Size:       4109
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4075
 
BciVI GTATCCnnnnn_n'

Cuts at:   2007    2768    4109    2007 
   Size:        761    1341    2007

  Fragments arranged by size: 

                2007    1341     761
 
BclI T'GATC_A

Cuts at:   1629    1629 
   Size:       4109
 
BglI GCCn_nnn'nGGC

Cuts at:    117     387     986     117 
   Size:        270     599    3240

  Fragments arranged by size: 

                3240     599     270
 
BglII A'GATC_T

Cuts at:   1624    3661    1624 
   Size:       2037    2072
 
BlpI GC'TnA_GC

Cuts at:    746    1221     746 
   Size:        475    3634
 
Bme1580I G_kGCm'C

Cuts at:    299     982    1019    1054    1734    1827    2883     299 
   Size:        683      37      35     680      93    1056    1525

  Fragments arranged by size: 

                1525    1056     683     680      93      37      35
 
BmrI ACTGGGnnnn_n'

Cuts at:    664    1062    1321    1402    1737    3881     664 
   Size:        398     259      81     335    2144     892

  Fragments arranged by size: 

                2144     892     398     335     259      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2476    2476 
   Size:       4109
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    217     531     600     217 
   Size:        314      69    3726

  Fragments arranged by size: 

                3726     314      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1285    2164    2656    2954    1285 
   Size:        879     492     298    2440

  Fragments arranged by size: 

                2440     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    153    3813     153 
   Size:       3660     449
 
BsaAI yAC'GTr

Cuts at:    897    1462     897 
   Size:        565    3544
 
BsaBI GATnn'nnATC

Cuts at:      6    1623    3660       6 
   Size:       1617    2037     455

  Fragments arranged by size: 

                2037    1617     455
 
BsaHI Gr'CG_yC

Cuts at:    470    1540    1788    2490    3986     470 
   Size:       1070     248     702    1496     593

  Fragments arranged by size: 

                1496    1070     702     593     248
 
BsaWI w'CCGG_w

Cuts at:   1298    1819    2497    2771    2918    3329    1298 
   Size:        521     678     274     147     411    2078

  Fragments arranged by size: 

                2078     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    529     559     529 
   Size:         30    4079
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1093    1093 
   Size:       4109
 
BseYI C'CCAG_C

Cuts at:    382     487     829    1534    2292    2869    3365     382 
   Size:        105     342     705     758     577     496    1126

  Fragments arranged by size: 

                1126     758     705     577     496     342     105
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    361     592     361 
   Size:        231    3878
 
BsiEI CG_ry'CG

Cuts at:    104    1651    1697    2905     104 
   Size:       1547      46    1208    1308

  Fragments arranged by size: 

                1547    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    428     664    1123    1901    2091    2883     428 
   Size:        236     459     778     190     792    1654

  Fragments arranged by size: 

                1654     792     778     459     236     190
 
BsmBI CGTCTCn'nnnn_

Cuts at:    461     461 
   Size:       4109
 
Bsp1286I G_dGCh'C

Cuts at:    299     383     428     664     982    1019    1054    1123    1734 
   Size:         84      45     236     318      37      35      69     611

Cuts at:   1734    1827    1901    2091    2153    2883     299 
   Size:         93      74     190      62     730    1525

  Fragments arranged by size: 

                1525     730     611     318     236     190      93      84
                  74      69      62      45      37      35
 
BspEI T'CCGG_A

Cuts at:   3329    3329 
   Size:       4109
 
BspHI T'CATG_A

Cuts at:    998    3285     998 
   Size:       2287    1822
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    159     360    1159    1373    1675    2056     159 
   Size:        201     799     214     302     381    2212

  Fragments arranged by size: 

                2212     799     381     302     214     201
 
BsrBI CCG'CTC

Cuts at:     50    2401    2455    3939    4104      50 
   Size:       2351      54    1484     165      55

  Fragments arranged by size: 

                2351    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2021    2021 
   Size:       4109
 
BsrFI r'CCGG_y

Cuts at:   2107    2288    2497    2107 
   Size:        181     209    3719

  Fragments arranged by size: 

                3719     209     181
 
BsrGI T'GTAC_A

Cuts at:    821     821 
   Size:       4109
 
BssSI C'ACGA_G

Cuts at:   2380    2738    2380 
   Size:        358    3751
 
BstAPI GCAn_nnn'nTGC

Cuts at:    484    1486     484 
   Size:       1002    3107
 
BstBI TT'CG_AA

Cuts at:   2470    2470 
   Size:       4109
 
BstXI CCAn_nnnn'nTGG

Cuts at:    250     250 
   Size:       4109
 
BstYI r'GATC_y

Cuts at:      1     406     833    1134    1601    1624    1959    2205    3217 
   Size:        405     427     301     467      23     335     246    1012

Cuts at:   3217    3303    3315    3661       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     427     405     346     335     301
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    249     249 
   Size:       4109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2059    2240    2403    2059 
   Size:        181     163    3765

  Fragments arranged by size: 

                3765     181     163
 
BtsI GCAGTG_nn'

Cuts at:    763    1063    1096    1352     763 
   Size:        300      33     256    3520

  Fragments arranged by size: 

                3520     300     256      33
 
DraI TTT'AAA

Cuts at:   1108    1108 
   Size:       4109
 
DraIII CAC_nnn'GTG

Cuts at:    900    1046     900 
   Size:        146    3963
 
DrdI GACnn_nn'nnGTC

Cuts at:    244    1815    2673     244 
   Size:       1571     858    1680

  Fragments arranged by size: 

                1680    1571     858
 
EaeI y'GGCC_r

Cuts at:    987    1694    1868    2259    2286    3631     987 
   Size:        707     174     391      27    1345    1465

  Fragments arranged by size: 

                1465    1345     707     391     174      27
 
EagI C'GGCC_G

Cuts at:   1694    1694 
   Size:       4109
 
EarI CTCTTCn'nnn_

Cuts at:    669    2132    2342     669 
   Size:       1463     210    2436

  Fragments arranged by size: 

                2436    1463     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2317    2627    2773    3919    2317 
   Size:        310     146    1146    2507

  Fragments arranged by size: 

                2507    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    426     662    1121     426 
   Size:        236     459    3414

  Fragments arranged by size: 

                3414     459     236
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    217     531     600    1934    2366    3113    3381     217 
   Size:        314      69    1334     432     747     268     945

  Fragments arranged by size: 

                1334     945     747     432     314     268      69
 
EcoO109I rG'GnC_Cy

Cuts at:    295     296     585     882    1248     295 
   Size:          1     289     297     366    3156

  Fragments arranged by size: 

                3156     366     297     289       1
 
EcoRI G'AATT_C

Cuts at:   1113    1113 
   Size:       4109
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2128    2128 
   Size:       4109
 
FspI TGC'GCA

Cuts at:   1890    1890 
   Size:       4109
 
HaeII r_GCGC'y

Cuts at:   1543    1791    2813    3792    1543 
   Size:        248    1022     979    1860

  Fragments arranged by size: 

                1860    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    394     426     682     714     818     850    1951    1981    1983 
   Size:         32     256      32     104      32    1101      30       2

Cuts at:   1983    2013     394 
   Size:         30    2490

  Fragments arranged by size: 

                2490    1101     256     104      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1148    1148 
   Size:       4109
 
KasI G'GCGC_C

Cuts at:   1539    1787    1539 
   Size:        248    3861
 
KpnI G_GTAC'C

Cuts at:   1129    1129 
   Size:       4109
 
MluI A'CGCG_T

Cuts at:   3542    3542 
   Size:       4109
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2780    2964    2780 
   Size:        184    3925
 
MscI TGG'CCA

Cuts at:    989    1870     989 
   Size:        881    3228
 
MslI CAynn'nnrTG

Cuts at:    614    2098    2225     614 
   Size:       1484     127    2498

  Fragments arranged by size: 

                2498    1484     127
 
MspA1I CmG'CkG

Cuts at:    386    1220    1534    1894    2907    3152     386 
   Size:        834     314     360    1013     245    1343

  Fragments arranged by size: 

                1343    1013     834     360     314     245
 
NaeI GCC'GGC

Cuts at:   2290    2290 
   Size:       4109
 
NarI GG'CG_CC

Cuts at:   1540    1788    1540 
   Size:        248    3861
 
NcoI C'CATG_G

Cuts at:    249     249 
   Size:       4109
 
NgoMIV G'CCGG_C

Cuts at:   2288    2288 
   Size:       4109
 
NspI r_CATG'y

Cuts at:   1162    1662    2569    1162 
   Size:        500     907    2702

  Fragments arranged by size: 

                2702     907     500
 
PasI CC'CwG_GG

Cuts at:    435     435 
   Size:       4109
 
PciI A'CATG_T

Cuts at:   2565    2565 
   Size:       4109
 
PfoI T'CCnGG_A

Cuts at:    233     233 
   Size:       4109
 
PmeI GTTT'AAAC

Cuts at:   1108    1108 
   Size:       4109
 
PmlI CAC'GTG

Cuts at:    897     897 
   Size:       4109
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    529     561    3248    3280     529 
   Size:         32    2687      32    1358

  Fragments arranged by size: 

                2687    1358      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    585     882     585 
   Size:        297    3812
 
PsiI TTA'TAA

Cuts at:    641     641 
   Size:       4109
 
PspOMI G'GGCC_C

Cuts at:    295    1015     295 
   Size:        720    3389
 
PstI C_TGCA'G

Cuts at:    221     423     480     926    1156    1841     221 
   Size:        202      57     446     230     685    2489

  Fragments arranged by size: 

                2489     685     446     230     202      57
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4109
 
PvuII CAG'CTG

Cuts at:   1534    1894    1534 
   Size:        360    3749
 
RsrII CG'GwC_CG

Cuts at:   2304    2304 
   Size:       4109
 
SacI G_AGCT'C

Cuts at:    428     664    1123     428 
   Size:        236     459    3414

  Fragments arranged by size: 

                3414     459     236
 
SalI G'TCGA_C

Cuts at:   1146    1146 
   Size:       4109
 
SanDI GG'GwC_CC

Cuts at:    585     585 
   Size:       4109
 
SapI GCTCTTCn'nnn_

Cuts at:    669    2132    2342     669 
   Size:       1463     210    2436

  Fragments arranged by size: 

                2436    1463     210
 
SbfI CC_TGCA'GG

Cuts at:    480    1156     480 
   Size:        676    3433
 
ScaI AGT'ACT

Cuts at:   3626    3626 
   Size:       4109
 
SexAI A'CCwGG_T

Cuts at:    992    1042     992 
   Size:         50    4059
 
SfcI C'TryA_G

Cuts at:     33     202     217     419     476     922    1152    1837    2830 
   Size:        169      15     202      57     446     230     685     993

Cuts at:   2830    3021      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     685     446     230     202     191     169
                  57      15
 
SfoI GGC'GCC

Cuts at:   1541    1789    1541 
   Size:        248    3861
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4109
 
SmaI CCC'GGG

Cuts at:   1131    1131 
   Size:       4109
 
SmlI C'TyrA_G

Cuts at:   1264    2179    2671    2933    1264 
   Size:        915     492     262    2440

  Fragments arranged by size: 

                2440     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4109
 
SphI G_CATG'C

Cuts at:   1162    1662    1162 
   Size:        500    3609
 
StyI C'CwwG_G

Cuts at:    249     936    1243    1597     249 
   Size:        687     307     354    2761

  Fragments arranged by size: 

                2761     687     354     307
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    256     522     696    1692    1825    2284    2493     256 
   Size:        266     174     996     133     459     209    1872

  Fragments arranged by size: 

                1872     996     459     266     209     174     133
 
TatI w'GTAC_w

Cuts at:    821     910    3624     821 
   Size:         89    2714    1306

  Fragments arranged by size: 

                2714    1306      89
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3626    3658    3626 
   Size:         32    4077
 
Tth111I GACn'n_nGTC

Cuts at:   1906    1906 
   Size:       4109
 
XbaI T'CTAG_A

Cuts at:    279    1140     279 
   Size:        861    3248
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1604    1604 
   Size:       4109
 
XmaI C'CCGG_G

Cuts at:   1129    1129 
   Size:       4109
 
XmnI GAAnn'nnTTC

Cuts at:    262     541     262 
   Size:        279    3830
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AlfI     AscI    AvrII     BaeI    BbvCI
    BfrBI    BmgBI     BmtI   Bpu10I    BsiWI     BsmI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI    PflMI    PshAI    PspXI     PsrI    SacII     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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