Restriction map for pF1KB8169
 (Linear) (Six-base) MAPSORT of: KIBB8169.seq  Check: 3027  from: 1  to: 996



KIBB8169.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      51     996 
   Size:         51     945
 
AccI GT'mk_AC

Cuts at:      0     854     996 
   Size:        854     142
 
AhdI GACnn_n'nnGTC

Cuts at:      0     588     996 
   Size:        588     408
 
AleI CACnn'nnGTG

Cuts at:      0     506     996 
   Size:        506     490
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     421     453     996 
   Size:        421      32     543

  Fragments arranged by size: 

                 543     421      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     996 
   Size:        117     879
 
ApaI G_GGCC'C

Cuts at:      0     191     911     996 
   Size:        191     720      85

  Fragments arranged by size: 

                 720     191      85
 
ApoI r'AATT_y

Cuts at:      0      25     996 
   Size:         25     971
 
AvaI C'yCGr_G

Cuts at:      0     486     996 
   Size:        486     510
 
BamHI G'GATC_C

Cuts at:      0     298     725     996 
   Size:        298     427     271

  Fragments arranged by size: 

                 427     298     271
 
BanI G'GyrC_C

Cuts at:      0     941     996 
   Size:        941      55
 
BanII G_rGCy'C

Cuts at:      0     191     275     320     556     911     996 
   Size:        191      84      45     236     355      85

  Fragments arranged by size: 

                 355     236     191      85      84      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     292     996 
   Size:        292     704
 
BglI GCCn_nnn'nGGC

Cuts at:      0       9     279     878     996 
   Size:          9     270     599     118

  Fragments arranged by size: 

                 599     270     118       9
 
BlpI GC'TnA_GC

Cuts at:      0     638     996 
   Size:        638     358
 
Bme1580I G_kGCm'C

Cuts at:      0     191     874     911     946     996 
   Size:        191     683      37      35      50

  Fragments arranged by size: 

                 683     191      50      37      35
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     556     954     996 
   Size:        556     398      42

  Fragments arranged by size: 

                 556     398      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     423     492     996 
   Size:        109     314      69     504

  Fragments arranged by size: 

                 504     314     109      69
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      45     996 
   Size:         45     951
 
BsaAI yAC'GTr

Cuts at:      0     789     996 
   Size:        789     207
 
BsaHI Gr'CG_yC

Cuts at:      0     362     996 
   Size:        362     634
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     421     451     996 
   Size:        421      30     545

  Fragments arranged by size: 

                 545     421      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     985     996 
   Size:        985      11
 
BseYI C'CCAG_C

Cuts at:      0     274     379     721     996 
   Size:        274     105     342     275

  Fragments arranged by size: 

                 342     275     274     105
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     484     996 
   Size:        253     231     512

  Fragments arranged by size: 

                 512     253     231
 
BsiHKAI G_wGCw'C

Cuts at:      0     320     556     996 
   Size:        320     236     440

  Fragments arranged by size: 

                 440     320     236
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     353     996 
   Size:        353     643
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     275     320     556     874     911     946     996 
   Size:        191      84      45     236     318      37      35      50

  Fragments arranged by size: 

                 318     236     191      84      50      45      37      35
            
 
BspHI T'CATG_A

Cuts at:      0     890     996 
   Size:        890     106
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51     252     996 
   Size:         51     201     744

  Fragments arranged by size: 

                 744     201      51
 
BsrGI T'GTAC_A

Cuts at:      0     713     996 
   Size:        713     283
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     376     996 
   Size:        376     620
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     142     996 
   Size:        142     854
 
BstYI r'GATC_y

Cuts at:      0     298     725     996 
   Size:        298     427     271

  Fragments arranged by size: 

                 427     298     271
 
BtgI C'CryG_G

Cuts at:      0     141     996 
   Size:        141     855
 
BtsI GCAGTG_nn'

Cuts at:      0     655     955     988     996 
   Size:        655     300      33       8

  Fragments arranged by size: 

                 655     300      33       8
 
DraIII CAC_nnn'GTG

Cuts at:      0     792     938     996 
   Size:        792     146      58

  Fragments arranged by size: 

                 792     146      58
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     136     996 
   Size:        136     860
 
EaeI y'GGCC_r

Cuts at:      0     879     996 
   Size:        879     117
 
EarI CTCTTCn'nnn_

Cuts at:      0     561     996 
   Size:        561     435
 
EcoICRI GAG'CTC

Cuts at:      0     318     554     996 
   Size:        318     236     442

  Fragments arranged by size: 

                 442     318     236
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     423     492     996 
   Size:        109     314      69     504

  Fragments arranged by size: 

                 504     314     109      69
 
EcoO109I rG'GnC_Cy

Cuts at:      0     187     188     477     774     996 
   Size:        187       1     289     297     222

  Fragments arranged by size: 

                 297     289     222     187       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318     574     606     710     742     996 
   Size:        286      32     256      32     104      32     254

  Fragments arranged by size: 

                 286     256     254     104      32      32      32
 
MscI TGG'CCA

Cuts at:      0     881     996 
   Size:        881     115
 
MslI CAynn'nnrTG

Cuts at:      0     506     996 
   Size:        506     490
 
MspA1I CmG'CkG

Cuts at:      0     278     996 
   Size:        278     718
 
NcoI C'CATG_G

Cuts at:      0     141     996 
   Size:        141     855
 
PasI CC'CwG_GG

Cuts at:      0     327     996 
   Size:        327     669
 
PfoI T'CCnGG_A

Cuts at:      0     125     996 
   Size:        125     871
 
PmlI CAC'GTG

Cuts at:      0     789     996 
   Size:        789     207
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     421     453     996 
   Size:        421      32     543

  Fragments arranged by size: 

                 543     421      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     477     774     996 
   Size:        477     297     222

  Fragments arranged by size: 

                 477     297     222
 
PsiI TTA'TAA

Cuts at:      0     533     996 
   Size:        533     463
 
PspOMI G'GGCC_C

Cuts at:      0     187     907     996 
   Size:        187     720      89

  Fragments arranged by size: 

                 720     187      89
 
PstI C_TGCA'G

Cuts at:      0     113     315     372     818     996 
   Size:        113     202      57     446     178

  Fragments arranged by size: 

                 446     202     178     113      57
 
SacI G_AGCT'C

Cuts at:      0     320     556     996 
   Size:        320     236     440

  Fragments arranged by size: 

                 440     320     236
 
SanDI GG'GwC_CC

Cuts at:      0     477     996 
   Size:        477     519
 
SapI GCTCTTCn'nnn_

Cuts at:      0     561     996 
   Size:        561     435
 
SbfI CC_TGCA'GG

Cuts at:      0     372     996 
   Size:        372     624
 
SexAI A'CCwGG_T

Cuts at:      0     884     934     996 
   Size:        884      50      62

  Fragments arranged by size: 

                 884      62      50
 
SfcI C'TryA_G

Cuts at:      0      94     109     311     368     814     996 
   Size:         94      15     202      57     446     182

  Fragments arranged by size: 

                 446     202     182      94      57      15
 
StyI C'CwwG_G

Cuts at:      0     141     828     996 
   Size:        141     687     168

  Fragments arranged by size: 

                 687     168     141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     148     414     588     996 
   Size:        148     266     174     408

  Fragments arranged by size: 

                 408     266     174     148
 
TatI w'GTAC_w

Cuts at:      0     713     802     996 
   Size:        713      89     194

  Fragments arranged by size: 

                 713     194      89
 
XbaI T'CTAG_A

Cuts at:      0     171     996 
   Size:        171     825
 
XmnI GAAnn'nnTTC

Cuts at:      0     154     433     996 
   Size:        154     279     563

  Fragments arranged by size: 

                 563     279     154
 

 Enzymes that do cut:

     AarI     AccI     AhdI     AleI     AloI    AlwNI     ApaI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI     BglI     BlpI Bme1580I
     BmrI     BpmI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrGI   BstAPI
    BstXI    BstYI     BtgI     BtsI   DraIII     DrdI     EaeI     EarI
  EcoICRI  Eco57MI EcoO109I    Hin4I     MscI     MslI   MspA1I     NcoI
     PasI     PfoI     PmlI     PpiI    PpuMI     PsiI   PspOMI     PstI
     SacI    SanDI     SapI     SbfI    SexAI     SfcI     StyI    TaqII
     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AlfI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmtI
     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI     EagI     EciI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI    PshAI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI
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