Restriction map for pF1KB8167
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AcyI        
AflIII      AgeI        AhdI        Alw44I      ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BcgI        BciVI       BclI        BfaI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsgI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       BstEII      BstPI       BstUI       
BstXI       BstYI       CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DsaI        EagI        
Eam1105I    EclXI       Eco52I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MflI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccIII        121  TCCGGA               T^CCGGA
AcsI          175  GAATTT               [GA]^AATT[CT]
AflII         595  CTTAAG               C^TTAAG
AocI          217  CCTGAGG              CC^T[ACGT]AGG
ApoI          175  GAATTT               [GA]^AATT[CT]
Asp700I       172  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         464  GAGCAC               G[AT]GC[AT]^C
BanI         1033  GGTGCC               G^G[CT][GA]CC
BbvCI         697  GCTGAGG              CCTCAGC(-5/-2)
BfrI          595  CTTAAG               C^TTAAG
BsaWI         121  TCCGGA               [AT]^CCGG[AT]
BseAI         121  TCCGGA               T^CCGGA
BsiHKAI       464  GAGCAC               G[AT]GC[AT]^C
BspEI         121  TCCGGA               T^CCGGA
BspHI          20  TCATGA               T^CATGA
BspMI        1134  GCAGGT               ACCTGC(4/8)
BsrDI          68  GCAATG               GCAATG(2/0)
Bst1107I     1063  GTATAC               GTA^TAC
BstAPI        781  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         611  TTCGAA               TT^CGAA
BstZ17I      1063  GTATAC               GTA^TAC
Bsu36I        217  CCTGAGG              CC^T[ACGT]AGG
DraIII       1177  CACCTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1151  GACGCTCAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1288  CGGCCA               [CT]^GGCC[GA]
Eco81I        217  CCTGAGG              CC^T[ACGT]AGG
EcoNI         927  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII         642  ACGT                 A^CGT
MroI          121  TCCGGA               T^CCGGA
MspA1I       1262  CAGCTG               C[AC]G^C[GT]G
NspI          917  GCATGC               [GA]CATG^[CT]
NspV          611  TTCGAA               TT^CGAA
PvuII        1262  CAGCTG               CAG^CTG
RcaI           20  TCATGA               T^CATGA
SfuI          611  TTCGAA               TT^CGAA
SphI          917  GCATGC               GCATG^C
XcmI          743  CCAAAAAAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          172  GAAGAATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI           42  GTCTAC               GT^[AC][GT]AC
             1063  GTATAC              
AlwNI         212  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              542  CAGAGTCTG           
AvaII           6  GGTCC                G^G[AT]CC
              621  GGACC               
BbsI          388  GAAGAC               GAAGAC(2/6)
              604  GTCTTC              
BbvI         1261  GCAGC                GCAGC(8/12)
              778  GCTGC               
BcnI          251  CCCGG                CC^[GC]GG
             1029  CCGGG               
BmyI          464  GAGCAC               G[AGT]GC[ACT]^C
             1229  GTGCCC              
BpuAI         388  GAAGAC               GAAGAC(2/6)
              604  GTCTTC              
Bsp1286I      464  GAGCAC               G[AGT]GC[ACT]^C
             1229  GTGCCC              
BsrBI         436  GAGCGG               CCGCTC(-3/-3)
             1285  GAGCGG              
BstNI         774  CCTGG                CC^[AT]GG
             1092  CCTGG               
Cac8I         462  GCGAGC               GC[ACGT]^[ACGT]GC
              917  GCATGC              
EcoRII        774  CCTGG                ^CC[AT]GG
             1092  CCTGG               
HgaI         1151  GACGC                GACGC(5/10)
              456  GCGTC               
MslI          498  CATGGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1027  CACCGGGGTG          
MvaI          774  CCTGG                CC^[AT]GG
             1092  CCTGG               
MwoI          781  GCATTGGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1154  GCTCAGGTCGC         
NciI          251  CCCGG                CC^[GC]GG
             1029  CCGGG               
TseI          778  GCTGC                G^C[AT]GC
             1261  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        Aor51HI     
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmFI       BsrI        BssKI       BstF5I      CfoI        
Cfr13I      DdeI        DpnII       EarI        Eco47III    
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MseI        MspI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       TthHB8I     
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