Restriction map for pF1KB8167
 (Linear) (Six-base) MAPSORT of: KIBB8167.seq  Check: 2171  from: 1  to: 1317



KIBB8167.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AccI GT'mk_AC

Cuts at:      0      43    1064    1317 
   Size:         43    1021     253

  Fragments arranged by size: 

                1021     253      43
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1317 
   Size:        350     967
 
AfeI AGC'GCT

Cuts at:      0      15     887    1083    1317 
   Size:         15     872     196     234

  Fragments arranged by size: 

                 872     234     196      15
 
AflII C'TTAA_G

Cuts at:      0     595    1317 
   Size:        595     722
 
AleI CACnn'nnGTG

Cuts at:      0    1031    1317 
   Size:       1031     286
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     754     786    1317 
   Size:        754      32     531

  Fragments arranged by size: 

                 754     531      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     217     547    1317 
   Size:        217     330     770

  Fragments arranged by size: 

                 770     330     217
 
ApoI r'AATT_y

Cuts at:      0     175    1317 
   Size:        175    1142
 
BanI G'GyrC_C

Cuts at:      0    1033    1317 
   Size:       1033     284
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     395     597    1317 
   Size:        395     202     720

  Fragments arranged by size: 

                 720     395     202
 
BbvCI CC'TCA_GC

Cuts at:      0     698    1317 
   Size:        698     619
 
Bme1580I G_kGCm'C

Cuts at:      0    1233    1317 
   Size:       1233      84
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      55    1317 
   Size:         55    1262
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     320     366    1317 
   Size:        320      46     951

  Fragments arranged by size: 

                 951     320      46
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     545     694    1114    1317 
   Size:        545     149     420     203

  Fragments arranged by size: 

                 545     420     203     149
 
Bpu10I CC'TnA_GC

Cuts at:      0     698    1155    1317 
   Size:        698     457     162

  Fragments arranged by size: 

                 698     457     162
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706     910    1317 
   Size:        706     204     407

  Fragments arranged by size: 

                 706     407     204
 
BsaWI w'CCGG_w

Cuts at:      0     121    1317 
   Size:        121    1196
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     178     244     421     424     544     832     957    1317 
   Size:        178      66     177       3     120     288     125     360

  Fragments arranged by size: 

                 360     288     178     177     125     120      66       3
            
 
BseYI C'CCAG_C

Cuts at:      0      49    1264    1317 
   Size:         49    1215      53

  Fragments arranged by size: 

                1215      53      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     468    1317 
   Size:        468     849
 
Bsp1286I G_dGCh'C

Cuts at:      0     468    1233    1317 
   Size:        468     765      84

  Fragments arranged by size: 

                 765     468      84
 
BspEI T'CCGG_A

Cuts at:      0     121    1317 
   Size:        121    1196
 
BspHI T'CATG_A

Cuts at:      0      20    1317 
   Size:         20    1297
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1125    1317 
   Size:       1125     192
 
BsrBI CCG'CTC

Cuts at:      0     438    1287    1317 
   Size:        438     849      30

  Fragments arranged by size: 

                 849     438      30
 
BsrDI GCAATG_nn'

Cuts at:      0      75    1317 
   Size:         75    1242
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     787    1317 
   Size:        787     530
 
BstBI TT'CG_AA

Cuts at:      0     612    1317 
   Size:        612     705
 
BstZ17I GTA'TAC

Cuts at:      0    1065    1317 
   Size:       1065     252
 
Bsu36I CC'TnA_GG

Cuts at:      0     218    1317 
   Size:        218    1099
 
BtsI GCAGTG_nn'

Cuts at:      0     402    1317 
   Size:        402     915
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1185    1220    1317 
   Size:       1185      35      97

  Fragments arranged by size: 

                1185      97      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1182    1317 
   Size:       1182     135
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1157    1317 
   Size:       1157     160
 
EaeI y'GGCC_r

Cuts at:      0    1288    1317 
   Size:       1288      29
 
EarI CTCTTCn'nnn_

Cuts at:      0     287     398     479     551     728     752     932    1317 
   Size:        287     111      81      72     177      24     180     385

  Fragments arranged by size: 

                 385     287     180     177     111      81      72      24
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     545     694    1114    1317 
   Size:        350     195     149     420     203

  Fragments arranged by size: 

                 420     350     203     195     149
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     931    1317 
   Size:        931     386
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     621    1317 
   Size:        621     696
 
HaeII r_GCGC'y

Cuts at:      0      17     889    1085    1317 
   Size:         17     872     196     232

  Fragments arranged by size: 

                 872     232     196      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255     523     555     573     605    1183    1215 
   Size:        223      32     268      32      18      32     578      32

Cuts at:   1215    1276    1308    1317 
   Size:         61      32       9

  Fragments arranged by size: 

                 578     268     223      61      32      32      32      32
                  32      18       9
 
MslI CAynn'nnrTG

Cuts at:      0     502    1031    1317 
   Size:        502     529     286

  Fragments arranged by size: 

                 529     502     286
 
MspA1I CmG'CkG

Cuts at:      0    1264    1317 
   Size:       1264      53
 
NspI r_CATG'y

Cuts at:      0     921    1317 
   Size:        921     396
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     617     649    1317 
   Size:        617      32     668

  Fragments arranged by size: 

                 668     617      32
 
PvuII CAG'CTG

Cuts at:      0    1264    1317 
   Size:       1264      53
 
SmlI C'TyrA_G

Cuts at:      0     595     721     889    1317 
   Size:        595     126     168     428

  Fragments arranged by size: 

                 595     428     168     126
 
SphI G_CATG'C

Cuts at:      0     921    1317 
   Size:        921     396
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     638    1317 
   Size:        638     679
 
TatI w'GTAC_w

Cuts at:      0     560     713    1317 
   Size:        560     153     604

  Fragments arranged by size: 

                 604     560     153
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     750    1317 
   Size:        750     567
 
XmnI GAAnn'nnTTC

Cuts at:      0     176    1317 
   Size:        176    1141
 

 Enzymes that do cut:

     AccI     AcuI     AfeI    AflII     AleI     AloI    AlwNI     ApoI
     BanI     BbsI    BbvCI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI   BstAPI    BstBI  BstZ17I   Bsu36I     BtsI
    CspCI   DraIII     DrdI     EaeI     EarI  Eco57MI    EcoNI     FalI
    HaeII    Hin4I     MslI   MspA1I     NspI     PpiI    PvuII     SmlI
     SphI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI   AflIII     AgeI     AhdI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI    BstYI     BtgI    BtgZI     ClaI
     DraI     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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