Restriction map for pF1KB8156
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BcgI        BciVI       
BfaI        BfrI        BglI        BlnI        BsaAI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FokI        
FseI        FspI        HaeII       HincII      HindII      
HindIII     HpaI        KasI        KpnI        MaeI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tsp45I      
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        1003  GACGTC               GACGT^C
AccII           6  CGCG                 CG^CG
AcyI         1003  GACGTC               G[GA]^CG[CT]C
AfaI          792  GTAC                 GT^AC
Alw44I        145  GTGCAC               G^TGCAC
AlwNI         319  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         145  GTGCAC               G^TGCAC
BbvCI        1015  GCTGAGG              CCTCAGC(-5/-2)
BclI          504  TGATCA               T^GATCA
BcnI          986  CCCGG                CC^[GC]GG
BlpI          808  GCTGAGC              GC^T[ACGT]AGC
BpmI          599  CTGGAG               CTGGAG(16/14)
Bpu1102I      808  GCTGAGC              GC^T[ACGT]AGC
BsaHI        1003  GACGTC               G[GA]^CG[CT]C
BsaJI           5  CCGCGG               C^C[ACGT][ACGT]GG
BsiYI         795  CCTATTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          795  CCTATTGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspMI         273  ACCTGC               ACCTGC(4/8)
BstUI           6  CGCG                 CG^CG
CelII         808  GCTGAGC              GC^T[ACGT]AGC
CfoI          112  GCGC                 GCG^C
DsaI            5  CCGCGG               C^C[GA][CT]GG
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EcoNI         795  CCTATTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          504  TGATCA               T^GATCA
HapII         987  CCGG                 C^CGG
HgaI          932  GACGC                GACGC(5/10)
HhaI          112  GCGC                 GCG^C
Hin1I        1003  GACGTC               G[GA]^CG[CT]C
HinP1I        112  GCGC                 G^CGC
HpaII         987  CCGG                 C^CGG
KspI            5  CCGCGG               CCGC^GG
MaeIII        193  GTTAC                ^GT[ACGT]AC
MslI          709  CATAAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          987  CCGG                 C^CGG
MvnI            6  CGCG                 CG^CG
NciI          986  CCCGG                CC^[GC]GG
NlaIV           9  GGTTCC               GG[ACGT]^[ACGT]CC
PaeR7I        305  CTCGAG               C^TCGAG
PstI           22  CTGCAG               CTGCA^G
RsaI          792  GTAC                 GT^AC
SacI          877  GAGCTC               GAGCT^C
SacII           5  CCGCGG               CCGC^GG
SapI          769  GAAGAGC              GCTCTTC(1/4)
SfcI           22  CTGCAG               C^T[GA][CT]AG
SmlI          305  CTCGAG               C^T[CT][GA]AG
XhoI          305  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Asp700I       430  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              636  GAAGAACTTC          
AspHI         145  GTGCAC               G[AT]GC[AT]^C
              877  GAGCTC              
AspI          127  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              414  GACTGTGTC           
AvaI           13  CCCGAG               C^[CT]CG[GA]G
              305  CTCGAG              
BbsI          208  GAAGAC               GAAGAC(2/6)
              411  GAAGAC              
BglII          55  AGATCT               A^GATCT
              703  AGATCT              
BpuAI         208  GAAGAC               GAAGAC(2/6)
              411  GAAGAC              
BsaBI          56  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              505  GATCAGGATC          
BseRI         880  CTCCTC               GAGGAG(10/8)
               34  GAGGAG              
BsiHKAI       145  GTGCAC               G[AT]GC[AT]^C
              877  GAGCTC              
BsmAI         125  GAGAC                GTCTC(1/5)
             1171  GTCTC               
BsoBI          13  CCCGAG               C^[CT]CG[GA]G
              305  CTCGAG              
Cac8I          47  GCAAGC               GC[ACGT]^[ACGT]GC
              745  GCCTGC              
EarI          769  GAAGAG               CTCTTC(1/4)
             1098  GAAGAG              
HaeIII          3  GGCC                 GG^CC
              984  GGCC                
Ksp632I       769  GAAGAG               CTCTTC(1/4)
             1098  GAAGAG              
MaeII         169  ACGT                 A^CGT
             1004  ACGT                
MamI           56  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              505  GATCAGGATC          
MspA1I          5  CCGCGG               C[AC]G^C[GT]G
              442  CAGCGG              
NspI          893  ACATGC               [GA]CATG^[CT]
             1155  ACATGC              
PflFI         127  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              414  GACTGTGTC           
SfaNI         109  GATGC                GCATC(5/9)
              315  GATGC               
TaqI          306  TCGA                 T^CGA
              843  TCGA                
Tth111I       127  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              414  GACTGTGTC           
TthHB8I       306  TCGA                 T^CGA
              843  TCGA                
XmnI          430  GAAGTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              636  GAAGAACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BanII       BbvI        BmyI        Bsp1286I    
BsrI        BssKI       BstNI       BstYI       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      HinfI       
HphI        ItaI        MboI        MboII       MflI        
MnlI        MseI        MvaI        MwoI        NdeII       
NlaIII      Sau3AI      Sau96I      ScrFI       TfiI        
Tru9I       TseI        Tsp509I     TspRI       XhoII       
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