Restriction map for pF1KB8147
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AfaI          210  GTAC                 GT^AC
AlwI           68  GGATC                GGATC(4/5)
AlwNI         119  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          517  CCTAAGG              CC^T[ACGT]AGG
ApaI          777  GGGCCC               GGGCC^C
AspHI         114  GTGCTC               G[AT]GC[AT]^C
AspI          389  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         777  GGGCCC               G[GA]GC[CT]^C
BbsI          622  GAAGAC               GAAGAC(2/6)
BlpI          193  GCTAAGC              GC^T[ACGT]AGC
BpmI          428  CTCCAG               CTGGAG(16/14)
Bpu1102I      193  GCTAAGC              GC^T[ACGT]AGC
BpuAI         622  GAAGAC               GAAGAC(2/6)
BseRI         787  GAGGAG               GAGGAG(10/8)
BsgI          470  GTGCAG               GTGCAG(16/14)
BsiHKAI       114  GTGCTC               G[AT]GC[AT]^C
BsmBI         566  GAGACG               CGTCTC(1/5)
BsrDI         165  CATTGC               GCAATG(2/0)
BsrI           32  CCAGT                ACTGG(1/-1)
BssSI         205  CACGAG               CACGAG(-5/-1)
Bst1107I       13  GTATAC               GTA^TAC
BstF5I        213  CATCC                GGATG(2/0)
BstZ17I        13  GTATAC               GTA^TAC
Bsu36I        517  CCTAAGG              CC^T[ACGT]AGG
CelII         193  GCTAAGC              GC^T[ACGT]AGC
DraII         776  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EarI          796  GAAGAG               CTCTTC(1/4)
Eco81I        517  CCTAAGG              CC^T[ACGT]AGG
EcoNI         766  CCTGATGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      776  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         316  GAATTC               G^AATTC
FokI          213  CATCC                GGATG(9/13)
Ksp632I       796  GAAGAG               CTCTTC(1/4)
MaeIII         28  GTGAC                ^GT[ACGT]AC
MseI          245  TTAA                 T^TAA
MwoI          514  GCTCCTAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI         389  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           76  GAGTC                GAGTC(4/5)
PvuII         578  CAGCTG               CAG^CTG
RsaI          210  GTAC                 GT^AC
SalI           47  GTCGAC               G^TCGAC
TfiI           90  GAATC                G^A[AT]TC
Tru9I         245  TTAA                 T^TAA
Tsp45I         28  GTGAC                ^GT[GC]AC
Tth111I       389  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI          506  CCGC                 CCGC(-3/-1)
              511  GCGG                
AcsI          184  AAATTC               [GA]^AATT[CT]
              316  GAATTC              
ApoI          184  AAATTC               [GA]^AATT[CT]
              316  GAATTC              
Asp700I       316  GAATTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              336  GAAGTTTTTC          
BfaI          199  CTAG                 C^TAG
              464  CTAG                
BmyI          114  GTGCTC               G[AGT]GC[ACT]^C
              777  GGGCCC              
BsmAI         566  GAGAC                GTCTC(1/5)
               78  GTCTC               
Bsp1286I      114  GTGCTC               G[AGT]GC[ACT]^C
              777  GGGCCC              
BspMI         238  GCAGGT               ACCTGC(4/8)
              594  GCAGGT              
Cac8I          60  GCTGGC               GC[ACGT]^[ACGT]GC
              523  GCTGGC              
DpnII          69  GATC                 ^GATC
              102  GATC                
HinfI          76  GAGTC                G^A[ACGT]TC
               90  GAATC               
MaeI          199  CTAG                 C^TAG
              464  CTAG                
MboI           69  GATC                 ^GATC
              102  GATC                
MspA1I        506  CCGCTG               C[AC]G^C[GT]G
              578  CAGCTG              
NdeII          69  GATC                 ^GATC
              102  GATC                
NspI           56  ACATGC               [GA]CATG^[CT]
              146  ACATGC              
PstI           82  CTGCAG               CTGCA^G
              404  CTGCAG              
Sau3AI         69  GATC                 ^GATC
              102  GATC                
SfaNI         346  GATGC                GCATC(5/9)
              588  GATGC               
SfcI           82  CTGCAG               C^T[GA][CT]AG
              404  CTGCAG              
XmnI          316  GAATTCGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              336  GAAGTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AccI        AluI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstNI       Cfr13I      DdeI        
EcoRII      Fnu4HI      HaeIII      HincII      HindII      
ItaI        MaeII       MboII       MnlI        MvaI        
NlaIII      NlaIV       Sau96I      ScrFI       TaqI        
TseI        Tsp509I     TspRI       TthHB8I     
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