Restriction map for pF1KB8140
 (Linear) (Six-base) MAPSORT of: KIBB8140.seq  Check: 1025  from: 1  to: 813



KIBB8140.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AatII G_ACGT'C

Cuts at:      0     283     813 
   Size:        283     530
 
AccI GT'mk_AC

Cuts at:      0     305     813 
   Size:        305     508
 
AclI AA'CG_TT

Cuts at:      0     232     813 
   Size:        232     581
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     386     526     813 
   Size:        361      25     140     287

  Fragments arranged by size: 

                 361     287     140      25
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     566     813 
   Size:        566     247
 
AlwNI CAG_nnn'CTG

Cuts at:      0      87     588     813 
   Size:         87     501     225

  Fragments arranged by size: 

                 501     225      87
 
ApoI r'AATT_y

Cuts at:      0     697     813 
   Size:        697     116
 
BanI G'GyrC_C

Cuts at:      0      40      49     813 
   Size:         40       9     764

  Fragments arranged by size: 

                 764      40       9
 
BanII G_rGCy'C

Cuts at:      0     769     813 
   Size:        769      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     147     392     813 
   Size:        147     245     421

  Fragments arranged by size: 

                 421     245     147
 
BlpI GC'TnA_GC

Cuts at:      0     668     813 
   Size:        668     145
 
Bme1580I G_kGCm'C

Cuts at:      0      52     813 
   Size:         52     761
 
BmgBI CAC'GTC

Cuts at:      0     762     813 
   Size:        762      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     610     751     813 
   Size:        610     141      62

  Fragments arranged by size: 

                 610     141      62
 
BsaHI Gr'CG_yC

Cuts at:      0     280     813 
   Size:        280     533
 
BsaWI w'CCGG_w

Cuts at:      0     170     813 
   Size:        170     643
 
BseYI C'CCAG_C

Cuts at:      0     634     672     813 
   Size:        634      38     141

  Fragments arranged by size: 

                 634     141      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     455     756     813 
   Size:        455     301      57

  Fragments arranged by size: 

                 455     301      57
 
BsmI GAATG_Cn'

Cuts at:      0     439     813 
   Size:        439     374
 
Bsp1286I G_dGCh'C

Cuts at:      0      52     769     813 
   Size:         52     717      44

  Fragments arranged by size: 

                 717      52      44
 
BspEI T'CCGG_A

Cuts at:      0     170     813 
   Size:        170     643
 
BsrBI CCG'CTC

Cuts at:      0     390     813 
   Size:        390     423
 
BsrDI GCAATG_nn'

Cuts at:      0     560     813 
   Size:        560     253
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      87     813 
   Size:         87     726
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     642     813 
   Size:        642     171
 
BstZ17I GTA'TAC

Cuts at:      0     306     813 
   Size:        306     507
 
BtgI C'CryG_G

Cuts at:      0       8     813 
   Size:          8     805
 
BtsI GCAGTG_nn'

Cuts at:      0     656     744     813 
   Size:        656      88      69

  Fragments arranged by size: 

                 656      88      69
 
EaeI y'GGCC_r

Cuts at:      0     357     813 
   Size:        357     456
 
EarI CTCTTCn'nnn_

Cuts at:      0     747     813 
   Size:        747      66
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     386     526     610     751     813 
   Size:        361      25     140      84     141      62

  Fragments arranged by size: 

                 361     141     140      84      62      25
 
EcoRI G'AATT_C

Cuts at:      0     697     813 
   Size:        697     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     751     783     813 
   Size:        751      32      30

  Fragments arranged by size: 

                 751      32      30
 
HincII GTy'rAC

Cuts at:      0     254     315     813 
   Size:        254      61     498

  Fragments arranged by size: 

                 498     254      61
 
HpaI GTT'AAC

Cuts at:      0     254     813 
   Size:        254     559
 
MspA1I CmG'CkG

Cuts at:      0      10     296     588     672     813 
   Size:         10     286     292      84     141

  Fragments arranged by size: 

                 292     286     141      84      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     786     813 
   Size:        786      27
 
PfoI T'CCnGG_A

Cuts at:      0     223     728     779     813 
   Size:        223     505      51      34

  Fragments arranged by size: 

                 505     223      51      34
 
PshAI GACnn'nnGTC

Cuts at:      0     151     813 
   Size:        151     662
 
PstI C_TGCA'G

Cuts at:      0      92     813 
   Size:         92     721
 
PvuII CAG'CTG

Cuts at:      0     296     588     672     813 
   Size:        296     292      84     141

  Fragments arranged by size: 

                 296     292     141      84
 
SacII CC_GC'GG

Cuts at:      0      11     813 
   Size:         11     802
 
SfcI C'TryA_G

Cuts at:      0      88     813 
   Size:         88     725
 
StuI AGG'CCT

Cuts at:      0     260     813 
   Size:        260     553
 
StyI C'CwwG_G

Cuts at:      0     743     813 
   Size:        743      70
 
ZraI GAC'GTC

Cuts at:      0     281     813 
   Size:        281     532
 

 Enzymes that do cut:

    AatII     AccI     AclI     AcuI     AlfI    AlwNI     ApoI     BanI
    BanII     BbsI     BlpI Bme1580I    BmgBI     BpmI    BsaHI    BsaWI
    BseYI     BsgI     BsmI Bsp1286I    BspEI    BsrBI    BsrDI   BstAPI
    BstXI  BstZ17I     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoRI
    Hin4I   HincII     HpaI   MspA1I    PflMI     PfoI    PshAI     PstI
    PvuII    SacII     SfcI     StuI     StyI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstYI   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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