Restriction map for pF1KB8137
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1286I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       FbaI        FseI        FspI        HapII       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        Ksp632I     KspI        MaeII       MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I        801  GGTACC               G^GTACC
AccII         948  CGCG                 CG^CG
AluI          774  AGCT                 AG^CT
AlwNI         556  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       727  AGCGCT               AGC^GCT
Asp718I       801  GGTACC               G^GTACC
BanI          801  GGTACC               G^G[CT][GA]CC
BsaI          997  GAGACC               GGTCTC(1/5)
BseRI         925  GAGGAG               GAGGAG(10/8)
Bsp1407I      412  TGTACA               T^GTACA
BspHI         455  TCATGA               T^CATGA
BspMI         613  ACCTGC               ACCTGC(4/8)
BsrGI         412  TGTACA               T^GTACA
BssKI        1023  CCAGG                ^CC[ACGT]GG
BstNI        1023  CCAGG                CC^[AT]GG
BstUI         948  CGCG                 CG^CG
Cac8I         651  GCCAGC               GC[ACGT]^[ACGT]GC
DraII         244  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          905  CGGCCA               [CT]^GGCC[GA]
Eco47III      727  AGCGCT               AGC^GCT
EcoO109I      244  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII       1023  CCAGG                ^CC[AT]GG
EcoT22I       526  ATGCAT               ATGCA^T
HaeII         727  AGCGCT               [GA]GCGC^[CT]
KpnI          801  GGTACC               GGTAC^C
MseI          193  TTAA                 T^TAA
MspA1I        773  CAGCTG               C[AC]G^C[GT]G
MvaI         1023  CCAGG                CC^[AT]GG
MvnI          948  CGCG                 CG^CG
NcoI          371  CCATGG               C^CATGG
NsiI          526  ATGCAT               ATGCA^T
PpuMI         244  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         113  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          115  CTGCAG               CTGCA^G
PvuII         773  CAGCTG               CAG^CTG
RcaI          455  TCATGA               T^CATGA
ScrFI        1023  CCAGG                CC^[ACGT]GG
SfcI          115  CTGCAG               C^T[GA][CT]AG
SmlI          992  CTCAAG               C^T[CT][GA]AG
SspBI         412  TGTACA               T^GTACA
SspI          540  AATATT               AAT^ATT
Tru9I         193  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcsI          169  GAATTT               [GA]^AATT[CT]
              699  AAATTC              
AcyI          154  GACGCC               G[GA]^CG[CT]C
              184  GACGCC              
ApoI          169  GAATTT               [GA]^AATT[CT]
              699  AAATTC              
AvaI          507  CCCGAG               C^[CT]CG[GA]G
              596  CCCGAG              
AvaII         220  GGACC                G^G[AT]CC
              245  GGACC               
BbsI          609  GAAGAC               GAAGAC(2/6)
              765  GAAGAC              
BfaI          520  CTAG                 C^TAG
              878  CTAG                
BpuAI         609  GAAGAC               GAAGAC(2/6)
              765  GAAGAC              
BsaHI         154  GACGCC               G[GA]^CG[CT]C
              184  GACGCC              
BsoBI         507  CCCGAG               C^[CT]CG[GA]G
              596  CCCGAG              
BstEII        288  GGTGACC              G^GT[ACGT]ACC
              375  GGTCACC             
BstPI         288  GGTGACC              G^GT[ACGT]ACC
              375  GGTCACC             
BstXI         344  CCATCACGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              908  CCAACGGCATGG        
CfoI          728  GCGC                 GCG^C
              949  GCGC                
DdeI          589  CTCAG                C^T[ACGT]AG
              784  CTGAG               
DpnII         429  GATC                 ^GATC
              451  GATC                
DsaI          371  CCATGG               C^C[GA][CT]GG
              967  CCGTGG              
EcoO65I       288  GGTGACC              G^GT[ACGT]ACC
              375  GGTCACC             
EcoT14I       371  CCATGG               C^C[AT][AT]GG
              404  CCAAGG              
FauI          946  CCCGC                CCCGC(4/6)
              985  CCCGC               
HaeIII         43  GGCC                 GG^CC
              906  GGCC                
HgaI          154  GACGC                GACGC(5/10)
              184  GACGC               
HhaI          728  GCGC                 GCG^C
              949  GCGC                
Hin1I         154  GACGCC               G[GA]^CG[CT]C
              184  GACGCC              
HinP1I        728  GCGC                 G^CGC
              949  GCGC                
MaeI          520  CTAG                 C^TAG
              878  CTAG                
MboI          429  GATC                 ^GATC
              451  GATC                
MslI          345  CATCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              529  CATTTGTGTG          
NdeII         429  GATC                 ^GATC
              451  GATC                
PleI          325  GAGTC                GAGTC(4/5)
              786  GAGTC               
Sau3AI        429  GATC                 ^GATC
              451  GATC                
SfaNI         901  GATGC                GCATC(5/9)
              515  GCATC               
StyI          371  CCATGG               C^C[AT][AT]GG
              404  CCAAGG              
TaqI          319  TCGA                 T^CGA
              568  TCGA                
TfiI           92  GAATC                G^A[AT]TC
              461  GAATC               
TthHB8I       319  TCGA                 T^CGA
              568  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AciI        AfaI        BbvI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsrI        BstF5I      
Cfr13I      Fnu4HI      FokI        HinfI       HphI        
ItaI        MaeIII      MboII       MnlI        MwoI        
NlaIII      NlaIV       RsaI        Sau96I      TseI        
Tsp45I      Tsp509I     TspRI       
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