Restriction map for pF1KB8134
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BclI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MroI        MseI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIII      NotI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     Tth111I     
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        671  GGTACC               G^GTACC
AccI           73  GTAGAC               GT^[AC][GT]AC
AlwI          780  GATCC                GGATC(4/5)
AlwNI         721  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           38  GGGCCC               GGGCC^C
Asp718I       671  GGTACC               G^GTACC
AvaI          617  CCCGAG               C^[CT]CG[GA]G
BalI          485  TGGCCA               TGG^CCA
BbsI          337  GAAGAC               GAAGAC(2/6)
BciVI         468  GGATAC               GTATCC(6/5)
BglI          103  GCCCCCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         337  GAAGAC               GAAGAC(2/6)
BsaI          644  GAGACC               GGTCTC(1/5)
BsmAI         644  GAGAC                GTCTC(1/5)
BsoBI         617  CCCGAG               C^[CT]CG[GA]G
BstYI         779  AGATCC               [GA]^GATC[CT]
CfoI          774  GCGC                 GCG^C
DpnII         780  GATC                 ^GATC
DsaI          767  CCGTGG               C^C[GA][CT]GG
EaeI          485  TGGCCA               [CT]^GGCC[GA]
EarI          639  GAAGAG               CTCTTC(1/4)
EcoNI         312  CCTCAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       126  CCTTGG               C^C[AT][AT]GG
HhaI          774  GCGC                 GCG^C
HinP1I        774  GCGC                 G^CGC
KpnI          671  GGTACC               GGTAC^C
Ksp632I       639  GAAGAG               CTCTTC(1/4)
MaeII         788  ACGT                 A^CGT
MaeIII          3  GTCAC                ^GT[ACGT]AC
MboI          780  GATC                 ^GATC
MflI          779  AGATCC               [GA]^GATC[CT]
MluNI         485  TGGCCA               TGG^CCA
MscI          485  TGGCCA               TGG^CCA
MspA1I        679  CAGCGG               C[AC]G^C[GT]G
NdeII         780  GATC                 ^GATC
NruI          741  TCGCGA               TCG^CGA
PleI          763  GAGTC                GAGTC(4/5)
Sau3AI        780  GATC                 ^GATC
SmlI          313  CTCAAG               C^T[CT][GA]AG
StyI          126  CCTTGG               C^C[AT][AT]GG
TaqI          806  TCGA                 T^CGA
TfiI          738  GAATC                G^A[AT]TC
Tsp45I          3  GTCAC                ^GT[GC]AC
TspRI         155  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       806  TCGA                 T^CGA
XhoII         779  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII         742  CGCG                 CG^CG
              775  CGCG                
AfaI          672  GTAC                 GT^AC
              786  GTAC                
BanI          256  GGCACC               G^G[CT][GA]CC
              671  GGTACC              
BbvI          552  GCAGC                GCAGC(8/12)
              842  GCTGC               
BfaI           53  CTAG                 C^TAG
              349  CTAG                
BsmFI         623  GGGAC                GGGAC(10/14)
              429  GTCCC               
BspMI         797  ACCTGC               ACCTGC(4/8)
              175  GCAGGT              
BstUI         742  CGCG                 CG^CG
              775  CGCG                
DdeI          203  CTGAG                C^T[ACGT]AG
              667  CTGAG               
HinfI         738  GAATC                G^A[ACGT]TC
              763  GAGTC               
HphI          178  GGTGA                GGTGA(8/7)
              697  GGTGA               
MaeI           53  CTAG                 C^TAG
              349  CTAG                
MvnI          742  CGCG                 CG^CG
              775  CGCG                
PflMI          49  CCAGCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              720  CCAGGGGCTGG         
RsaI          672  GTAC                 GT^AC
              786  GTAC                
SfaNI         352  GATGC                GCATC(5/9)
              528  GATGC               
TseI          552  GCAGC                G^C[AT]GC
              842  GCTGC               
Van91I         49  CCAGCTAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              720  CCAGGGGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HpaII       ItaI        MboII       MnlI        
MspI        MvaI        MwoI        NciI        NlaIV       
PpuMI       Sau96I      ScrFI       
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