Restriction map for pF1KB8129
 (Linear) (Six-base) MAPSORT of: KIBB8129.seq  Check: 586  from: 1  to: 1023



KIBB8129.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      99    1023 
   Size:         99     924
 
AccI GT'mk_AC

Cuts at:      0     768    1023 
   Size:        768     255
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     517    1023 
   Size:         98     419     506

  Fragments arranged by size: 

                 506     419      98
 
AflIII A'CryG_T

Cuts at:      0     760    1023 
   Size:        760     263
 
AhdI GACnn_n'nnGTC

Cuts at:      0     764    1023 
   Size:        764     259
 
AlwNI CAG_nnn'CTG

Cuts at:      0     199     894     957     994    1023 
   Size:        199     695      63      37      29

  Fragments arranged by size: 

                 695     199      63      37      29
 
ApaLI G'TGCA_C

Cuts at:      0     315    1023 
   Size:        315     708
 
ApoI r'AATT_y

Cuts at:      0     541    1023 
   Size:        541     482
 
AvaI C'yCGr_G

Cuts at:      0     254     746    1023 
   Size:        254     492     277

  Fragments arranged by size: 

                 492     277     254
 
AvrII C'CTAG_G

Cuts at:      0      15    1023 
   Size:         15    1008
 
BamHI G'GATC_C

Cuts at:      0      11    1023 
   Size:         11    1012
 
BanII G_rGCy'C

Cuts at:      0     172    1023 
   Size:        172     851
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     662    1023 
   Size:        662     361
 
BbvCI CC'TCA_GC

Cuts at:      0     511    1023 
   Size:        511     512
 
Bme1580I G_kGCm'C

Cuts at:      0     319     447     783    1023 
   Size:        319     128     336     240

  Fragments arranged by size: 

                 336     319     240     128
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     499    1023 
   Size:        499     524
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1020    1023 
   Size:       1020       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     112     260     287     391     967     971    1023 
   Size:         88      24     148      27     104     576       4      52

  Fragments arranged by size: 

                 576     148     104      88      52      27      24       4
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     511    1023 
   Size:        511     512
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     832    1023 
   Size:        793      39     191

  Fragments arranged by size: 

                 793     191      39
 
BsaAI yAC'GTr

Cuts at:      0     763    1023 
   Size:        763     260
 
BsaBI GATnn'nnATC

Cuts at:      0      43    1023 
   Size:         43     980
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     233     263    1023 
   Size:        233      30     760

  Fragments arranged by size: 

                 760     233      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     742    1023 
   Size:        742     281
 
BseYI C'CCAG_C

Cuts at:      0     435     479     928    1023 
   Size:        435      44     449      95

  Fragments arranged by size: 

                 449     435      95      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     319    1023 
   Size:        319     704
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     397    1023 
   Size:        397     626
 
Bsp1286I G_dGCh'C

Cuts at:      0     172     319     447     783    1023 
   Size:        172     147     128     336     240

  Fragments arranged by size: 

                 336     240     172     147     128
 
BspHI T'CATG_A

Cuts at:      0     665    1023 
   Size:        665     358
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      99    1023 
   Size:         99     924
 
BsrDI GCAATG_nn'

Cuts at:      0     260    1023 
   Size:        260     763
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     894     957    1023 
   Size:        894      63      66

  Fragments arranged by size: 

                 894      66      63
 
BstEII G'GTnAC_C

Cuts at:      0     639    1023 
   Size:        639     384
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     651    1023 
   Size:        651     372
 
BstYI r'GATC_y

Cuts at:      0      11      38    1023 
   Size:         11      27     985

  Fragments arranged by size: 

                 985      27      11
 
BtgI C'CryG_G

Cuts at:      0      48     650    1023 
   Size:         48     602     373

  Fragments arranged by size: 

                 602     373      48
 
DraIII CAC_nnn'GTG

Cuts at:      0     493    1023 
   Size:        493     530
 
EaeI y'GGCC_r

Cuts at:      0     201     836    1023 
   Size:        201     635     187

  Fragments arranged by size: 

                 635     201     187
 
EarI CTCTTCn'nnn_

Cuts at:      0     904    1023 
   Size:        904     119
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     906    1023 
   Size:        906     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88      98     112     260     287     391     517     967 
   Size:         88      10      14     148      27     104     126     450

Cuts at:    967     971    1023 
   Size:          4      52

  Fragments arranged by size: 

                 450     148     126     104      88      52      27      14
                  10       4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      19     106    1023 
   Size:         19      87     917

  Fragments arranged by size: 

                 917      87      19
 
EcoO109I rG'GnC_Cy

Cuts at:      0      99     124     742    1023 
   Size:         99      25     618     281

  Fragments arranged by size: 

                 618     281      99      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     116     148     750     782    1023 
   Size:        116      32     602      32     241

  Fragments arranged by size: 

                 602     241     116      32      32
 
HincII GTy'rAC

Cuts at:      0     769    1023 
   Size:        769     254
 
MscI TGG'CCA

Cuts at:      0     838    1023 
   Size:        838     185
 
MslI CAynn'nnrTG

Cuts at:      0     664     670    1023 
   Size:        664       6     353

  Fragments arranged by size: 

                 664     353       6
 
MspA1I CmG'CkG

Cuts at:      0     435     891     945    1023 
   Size:        435     456      54      78

  Fragments arranged by size: 

                 456     435      78      54
 
NcoI C'CATG_G

Cuts at:      0     650    1023 
   Size:        650     373
 
NspI r_CATG'y

Cuts at:      0     487    1023 
   Size:        487     536
 
PfoI T'CCnGG_A

Cuts at:      0     854     987    1023 
   Size:        854     133      36

  Fragments arranged by size: 

                 854     133      36
 
PmlI CAC'GTG

Cuts at:      0     763    1023 
   Size:        763     260
 
PpuMI rG'GwC_Cy

Cuts at:      0      99     124    1023 
   Size:         99      25     899

  Fragments arranged by size: 

                 899      99      25
 
PstI C_TGCA'G

Cuts at:      0     386     434     476     962    1023 
   Size:        386      48      42     486      61

  Fragments arranged by size: 

                 486     386      61      48      42
 
PvuII CAG'CTG

Cuts at:      0     435     891     945    1023 
   Size:        435     456      54      78

  Fragments arranged by size: 

                 456     435      78      54
 
SalI G'TCGA_C

Cuts at:      0     767    1023 
   Size:        767     256
 
SapI GCTCTTCn'nnn_

Cuts at:      0     904    1023 
   Size:        904     119
 
SfcI C'TryA_G

Cuts at:      0     382     430     462     472     958    1023 
   Size:        382      48      32      10     486      65

  Fragments arranged by size: 

                 486     382      65      48      32      10
 
SmaI CCC'GGG

Cuts at:      0     748    1023 
   Size:        748     275
 
SmlI C'TyrA_G

Cuts at:      0     772     847    1023 
   Size:        772      75     176

  Fragments arranged by size: 

                 772     176      75
 
SphI G_CATG'C

Cuts at:      0     487    1023 
   Size:        487     536
 
StyI C'CwwG_G

Cuts at:      0      15     650    1023 
   Size:         15     635     373

  Fragments arranged by size: 

                 635     373      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     481    1023 
   Size:        481     542
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     233     265    1023 
   Size:        233      32     758

  Fragments arranged by size: 

                 758     233      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     283    1023 
   Size:        283     740
 
XmaI C'CCGG_G

Cuts at:      0     746    1023 
   Size:        746     277
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI    AlwNI    ApaLI     ApoI
     AvaI    AvrII    BamHI    BanII     BbsI    BbvCI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BseRI
    BseYI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI
   BstEII    BstXI    BstYI     BtgI   DraIII     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MscI     MslI   MspA1I
     NcoI     NspI     PfoI     PmlI    PpuMI     PstI    PvuII     SalI
     SapI     SfcI     SmaI     SmlI     SphI     StyI    TaqII     TstI
  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI     AscI     AseI    AsiSI     BaeI     BanI     BbeI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BsaI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EagI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI     TatI     XbaI     XcmI     XhoI
     XmnI     ZraI
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