Restriction map for
pF1KB8128
(Circular) (Six-base) MAPSORT of: pF1KB8128.seq Check: 8411 from: 1 to: 4037
pF1KB8128 4037 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 662 1075 662
Size: 413 3624
Acc65I G'GTAC_C
Cuts at: 1053 1053
Size: 4037
AclI AA'CG_TT
Cuts at: 3877 3877
Size: 4037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 824 1862 2294 3041 3309 501
Size: 323 1038 432 747 268 1229
Fragments arranged by size:
1229 1038 747 432 323 268
AflIII A'CryG_T
Cuts at: 1389 2493 3470 1389
Size: 1104 977 1956
Fragments arranged by size:
1956 1104 977
AgeI A'CCGG_T
Cuts at: 2425 2425
Size: 4037
AlwNI CAG_nnn'CTG
Cuts at: 1342 2909 1342
Size: 1567 2470
ApaI G_GGCC'C
Cuts at: 223 223
Size: 4037
ApaLI G'TGCA_C
Cuts at: 2807 2807
Size: 4037
ApoI r'AATT_y
Cuts at: 457 606 723 746 1041 3390 3646 457
Size: 149 117 23 295 2349 256 848
Fragments arranged by size:
2349 848 295 256 149 117 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4037
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
AvaI C'yCGr_G
Cuts at: 361 1057 361
Size: 696 3341
BamHI G'GATC_C
Cuts at: 1062 1062
Size: 4037
BanI G'GyrC_C
Cuts at: 1053 1467 1715 1750 1053
Size: 414 248 35 3340
Fragments arranged by size:
3340 414 248 35
BanII G_rGCy'C
Cuts at: 223 852 1051 2081 223
Size: 629 199 1030 2179
Fragments arranged by size:
2179 1030 629 199
BbeI G_GCGC'C
Cuts at: 1471 1719 1471
Size: 248 3789
BciVI GTATCCnnnnn_n'
Cuts at: 350 842 1935 2696 4037 350
Size: 492 1093 761 1341 350
Fragments arranged by size:
1341 1093 761 492 350
BclI T'GATC_A
Cuts at: 783 1557 783
Size: 774 3263
BglII A'GATC_T
Cuts at: 1552 3589 1552
Size: 2037 2000
BlpI GC'TnA_GC
Cuts at: 1149 1149
Size: 4037
Bme1580I G_kGCm'C
Cuts at: 223 1662 1755 2811 223
Size: 1439 93 1056 1449
Fragments arranged by size:
1449 1439 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1249 1330 1665 3809 1249
Size: 81 335 2144 1477
Fragments arranged by size:
2144 1477 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4037
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1213 2092 2584 2882 1009
Size: 204 879 492 298 2164
Fragments arranged by size:
2164 879 492 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 3741 3741
Size: 4037
BsaAI yAC'GTr
Cuts at: 1390 1390
Size: 4037
BsaBI GATnn'nnATC
Cuts at: 6 1551 3588 6
Size: 1545 2037 455
Fragments arranged by size:
2037 1545 455
BsaHI Gr'CG_yC
Cuts at: 1468 1716 2418 3914 1468
Size: 248 702 1496 1591
Fragments arranged by size:
1591 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1226 1747 2425 2699 2846 3257 1226
Size: 521 678 274 147 411 2006
Fragments arranged by size:
2006 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 997 1039 997
Size: 42 3995
BseYI C'CCAG_C
Cuts at: 1462 2220 2797 3293 1462
Size: 758 577 496 2206
Fragments arranged by size:
2206 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 906 914 1015 906
Size: 8 101 3928
Fragments arranged by size:
3928 101 8
BsiEI CG_ry'CG
Cuts at: 104 1579 1625 2833 104
Size: 1475 46 1208 1308
Fragments arranged by size:
1475 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 852 1051 1829 2019 2811 852
Size: 199 778 190 792 2078
Fragments arranged by size:
2078 792 778 199 190
Bsp1286I G_dGCh'C
Cuts at: 223 852 1051 1662 1755 1829 2019 2081 2811
Size: 629 199 611 93 74 190 62 730
Cuts at: 2811 223
Size: 1449
Fragments arranged by size:
1449 730 629 611 199 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3257 3257
Size: 4037
BspHI T'CATG_A
Cuts at: 3213 3213
Size: 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1020 1087 1301 1603 1984 1020
Size: 67 214 302 381 3073
Fragments arranged by size:
3073 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 2329 2383 3867 4032 50
Size: 2279 54 1484 165 55
Fragments arranged by size:
2279 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1949 1949
Size: 4037
BsrFI r'CCGG_y
Cuts at: 2035 2216 2425 2035
Size: 181 209 3647
Fragments arranged by size:
3647 209 181
BsrGI T'GTAC_A
Cuts at: 821 912 821
Size: 91 3946
BssSI C'ACGA_G
Cuts at: 2308 2666 2308
Size: 358 3679
BstAPI GCAn_nnn'nTGC
Cuts at: 1414 1414
Size: 4037
BstBI TT'CG_AA
Cuts at: 2398 2398
Size: 4037
BstEII G'GTnAC_C
Cuts at: 316 316
Size: 4037
BstXI CCAn_nnnn'nTGG
Cuts at: 350 350
Size: 4037
BstYI r'GATC_y
Cuts at: 1 1062 1529 1552 1887 2133 3145 3231 3243
Size: 1061 467 23 335 246 1012 86 12
Cuts at: 3243 3589 1
Size: 346 449
Fragments arranged by size:
1061 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 936 107
Size: 829 3208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1987 2168 2331 1987
Size: 181 163 3693
Fragments arranged by size:
3693 181 163
BtsI GCAGTG_nn'
Cuts at: 1280 1280
Size: 4037
DraI TTT'AAA
Cuts at: 1036 1036
Size: 4037
DrdI GACnn_nn'nnGTC
Cuts at: 1743 2601 1743
Size: 858 3179
EaeI y'GGCC_r
Cuts at: 953 1622 1796 2187 2214 3559 953
Size: 669 174 391 27 1345 1431
Fragments arranged by size:
1431 1345 669 391 174 27
EagI C'GGCC_G
Cuts at: 1622 1622
Size: 4037
EarI CTCTTCn'nnn_
Cuts at: 840 2060 2270 840
Size: 1220 210 2607
Fragments arranged by size:
2607 1220 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2245 2555 2701 3847 2245
Size: 310 146 1146 2435
Fragments arranged by size:
2435 1146 310 146
EcoICRI GAG'CTC
Cuts at: 850 1049 850
Size: 199 3838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 501 824 1862 2294 3041 3309 501
Size: 323 1038 432 747 268 1229
Fragments arranged by size:
1229 1038 747 432 323 268
EcoO109I rG'GnC_Cy
Cuts at: 220 1176 220
Size: 956 3081
EcoRI G'AATT_C
Cuts at: 1041 1041
Size: 4037
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 436 1024 2056 436
Size: 588 1032 2417
Fragments arranged by size:
2417 1032 588
FspI TGC'GCA
Cuts at: 1818 1818
Size: 4037
HaeII r_GCGC'y
Cuts at: 431 1471 1719 2741 3720 431
Size: 1040 248 1022 979 748
Fragments arranged by size:
1040 1022 979 748 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 285 317 1879 1909 1911 1941 285
Size: 32 1562 30 2 30 2381
Fragments arranged by size:
2381 1562 32 30 30 2
HincII GTy'rAC
Cuts at: 1076 1076
Size: 4037
KasI G'GCGC_C
Cuts at: 1467 1715 1467
Size: 248 3789
KpnI G_GTAC'C
Cuts at: 1057 1057
Size: 4037
MfeI C'AATT_G
Cuts at: 403 403
Size: 4037
MluI A'CGCG_T
Cuts at: 3470 3470
Size: 4037
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2708 2892 2708
Size: 184 3853
MscI TGG'CCA
Cuts at: 1798 1798
Size: 4037
MslI CAynn'nnrTG
Cuts at: 882 2026 2153 882
Size: 1144 127 2766
Fragments arranged by size:
2766 1144 127
MspA1I CmG'CkG
Cuts at: 1148 1462 1822 2835 3080 1148
Size: 314 360 1013 245 2105
Fragments arranged by size:
2105 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2218 2218
Size: 4037
NarI GG'CG_CC
Cuts at: 1468 1716 1468
Size: 248 3789
NcoI C'CATG_G
Cuts at: 107 936 107
Size: 829 3208
NgoMIV G'CCGG_C
Cuts at: 2216 2216
Size: 4037
NspI r_CATG'y
Cuts at: 1090 1590 2497 1090
Size: 500 907 2630
Fragments arranged by size:
2630 907 500
PciI A'CATG_T
Cuts at: 2493 2493
Size: 4037
PmeI GTTT'AAAC
Cuts at: 1036 1036
Size: 4037
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 140 172 3176 3208 140
Size: 32 3004 32 969
Fragments arranged by size:
3004 969 32 32
PsiI TTA'TAA
Cuts at: 137 137
Size: 4037
PspOMI G'GGCC_C
Cuts at: 219 219
Size: 4037
PstI C_TGCA'G
Cuts at: 276 1084 1769 276
Size: 808 685 2544
Fragments arranged by size:
2544 808 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4037
PvuII CAG'CTG
Cuts at: 1462 1822 1462
Size: 360 3677
RsrII CG'GwC_CG
Cuts at: 2232 2232
Size: 4037
SacI G_AGCT'C
Cuts at: 852 1051 852
Size: 199 3838
SalI G'TCGA_C
Cuts at: 1074 1074
Size: 4037
SapI GCTCTTCn'nnn_
Cuts at: 840 2060 2270 840
Size: 1220 210 2607
Fragments arranged by size:
2607 1220 210
SbfI CC_TGCA'GG
Cuts at: 1084 1084
Size: 4037
ScaI AGT'ACT
Cuts at: 3554 3554
Size: 4037
SfcI C'TryA_G
Cuts at: 33 272 1080 1765 2758 2949 33
Size: 239 808 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 239 191
SfoI GGC'GCC
Cuts at: 1469 1717 1469
Size: 248 3789
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4037
SmaI CCC'GGG
Cuts at: 1059 1059
Size: 4037
SmlI C'TyrA_G
Cuts at: 361 988 1192 2107 2599 2861 361
Size: 627 204 915 492 262 1537
Fragments arranged by size:
1537 915 627 492 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4037
SphI G_CATG'C
Cuts at: 1090 1590 1090
Size: 500 3537
StyI C'CwwG_G
Cuts at: 107 936 1008 1171 1525 107
Size: 829 72 163 354 2619
Fragments arranged by size:
2619 829 354 163 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1620 1753 2212 2421 1620
Size: 133 459 209 3236
Fragments arranged by size:
3236 459 209 133
TatI w'GTAC_w
Cuts at: 821 912 3552 821
Size: 91 2640 1306
Fragments arranged by size:
2640 1306 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3554 3586 3554
Size: 32 4005
Tth111I GACn'n_nGTC
Cuts at: 1834 1834
Size: 4037
XbaI T'CTAG_A
Cuts at: 1068 1068
Size: 4037
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 654 1532 654
Size: 878 3159
XhoI C'TCGA_G
Cuts at: 361 361
Size: 4037
XmaI C'CCGG_G
Cuts at: 1057 1057
Size: 4037
XmnI GAAnn'nnTTC
Cuts at: 609 609
Size: 4037
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BsiWI BsmI BsmBI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI ZraI