Restriction map for pF1KB8122
##################################
# NO CUT ENZYMES (  160 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiWI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TaqI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         181  CGCG                 CG^CG
AciI           81  GCGG                 CCGC(-3/-1)
AclI          356  AACGTT               AA^CGTT
AlwI          189  GATCC                GGATC(4/5)
BalI          348  TGGCCA               TGG^CCA
BanI          501  GGTGCC               G^G[CT][GA]CC
BcnI          318  CCGGG                CC^[GC]GG
BsaI          270  GGTCTC               GGTCTC(1/5)
BsgI          286  GTGCAG               GTGCAG(16/14)
BsiEI          44  CGACCG               CG[GA][CT]^CG
BsrDI         237  CATTGC               GCAATG(2/0)
BsrFI         228  GCCGGT               [GA]^CCGG[CT]
BstEII        231  GGTCACC              G^GT[ACGT]ACC
BstPI         231  GGTCACC              G^GT[ACGT]ACC
BstUI         181  CGCG                 CG^CG
BstYI         188  AGATCC               [GA]^GATC[CT]
CfoI           14  GCGC                 GCG^C
Cfr10I        228  GCCGGT               [GA]^CCGG[CT]
DpnII         189  GATC                 ^GATC
DraII         330  AGGACCC              [GA]G^G[ACGT]CC[CT]
EaeI          348  TGGCCA               [CT]^GGCC[GA]
EcoO109I      330  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       231  GGTCACC              G^GT[ACGT]ACC
EcoT22I       175  ATGCAT               ATGCA^T
HaeII          13  AGCGCC               [GA]GCGC^[CT]
HhaI           14  GCGC                 GCG^C
HinP1I         14  GCGC                 G^CGC
HphI          233  TCACC                GGTGA(8/7)
MaeII         357  ACGT                 A^CGT
MboI          189  GATC                 ^GATC
MflI          188  AGATCC               [GA]^GATC[CT]
MluNI         348  TGGCCA               TGG^CCA
MscI          348  TGGCCA               TGG^CCA
MspA1I        103  CAGCTG               C[AC]G^C[GT]G
MvnI          181  CGCG                 CG^CG
NciI          318  CCGGG                CC^[GC]GG
NdeII         189  GATC                 ^GATC
NsiI          175  ATGCAT               ATGCA^T
PpuMI         330  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      356  AACGTT               AA^CGTT
PstI          123  CTGCAG               CTGCA^G
PvuII         103  CAGCTG               CAG^CTG
SacI           64  GAGCTC               GAGCT^C
Sau3AI        189  GATC                 ^GATC
SfcI          123  CTGCAG               C^T[GA][CT]AG
StuI          251  AGGCCT               AGG^CCT
XhoII         188  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AspHI          49  GTGCTC               G[AT]GC[AT]^C
               64  GAGCTC              
AvaII          89  GGACC                G^G[AT]CC
              331  GGACC               
BanII          64  GAGCTC               G[GA]GC[CT]^C
              585  GAGCCC              
BsiHKAI        49  GTGCTC               G[AT]GC[AT]^C
               64  GAGCTC              
BsmAI         578  GAGAC                GTCTC(1/5)
              271  GTCTC               
BsrI           17  CCAGT                ACTGG(1/-1)
              301  CCAGT               
BstF5I        441  GGATG                GGATG(2/0)
              447  GGATG               
Cfr13I         89  GGACC                G^G[ACGT]CC
              331  GGACC               
DsaI          266  CCATGG               C^C[GA][CT]GG
              275  CCATGG              
EcoT14I       266  CCATGG               C^C[AT][AT]GG
              275  CCATGG              
FokI          441  GGATG                GGATG(9/13)
              447  GGATG               
HapII         229  CCGG                 C^CGG
              318  CCGG                
HinfI           3  GAATC                G^A[ACGT]TC
              632  GATTC               
HpaII         229  CCGG                 C^CGG
              318  CCGG                
MspI          229  CCGG                 C^CGG
              318  CCGG                
NcoI          266  CCATGG               C^CATGG
              275  CCATGG              
NlaIII        267  CATG                 CATG^
              276  CATG                
NlaIV         331  GGACCC               GG[ACGT]^[ACGT]CC
              501  GGTGCC              
Sau96I         89  GGACC                G^G[ACGT]CC
              331  GGACC               
StyI          266  CCATGG               C^C[AT][AT]GG
              275  CCATGG              
TfiI            3  GAATC                G^A[AT]TC
              632  GATTC               
Tsp45I         40  GTGAC                ^GT[GC]AC
              232  GTCAC               
TspRI          38  CAGTG                CA[GC]TG[ACGT][ACGT]^
              345  CAGTG               
XcmI          266  CCATGGTCTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              312  CCAGCTCCGGGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AluI        BbvI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        Bsp1286I    BssKI       
BstNI       Cac8I       DdeI        EarI        EcoRII      
Fnu4HI      HaeIII      ItaI        Ksp632I     MaeIII      
MboII       MnlI        MvaI        MwoI        ScrFI       
SfaNI       TseI        
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