(Circular) (Six-base) MAPSORT of: pF1KB8121.seq Check: 2720 from: 1 to: 4073 pF1KB8121 4073 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 1111 1111 Size: 4073 Acc65I G'GTAC_C Cuts at: 1089 1089 Size: 4073 AclI AA'CG_TT Cuts at: 3913 3913 Size: 4073 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 476 756 1898 2330 3077 3345 476 Size: 280 1142 432 747 268 1204 Fragments arranged by size: 1204 1142 747 432 280 268 AfeI AGC'GCT Cuts at: 265 265 Size: 4073 AflIII A'CryG_T Cuts at: 1425 2529 3506 1425 Size: 1104 977 1992 Fragments arranged by size: 1992 1104 977 AgeI A'CCGG_T Cuts at: 2461 2461 Size: 4073 AleI CACnn'nnGTG Cuts at: 629 629 Size: 4073 AlwNI CAG_nnn'CTG Cuts at: 1378 2945 1378 Size: 1567 2506 ApaLI G'TGCA_C Cuts at: 2843 2843 Size: 4073 ApoI r'AATT_y Cuts at: 921 945 1077 3426 3682 921 Size: 24 132 2349 256 1312 Fragments arranged by size: 2349 1312 256 132 24 AseI AT'TA_AT Cuts at: 20 20 Size: 4073 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4073 AvaI C'yCGr_G Cuts at: 1093 1093 Size: 4073 BamHI G'GATC_C Cuts at: 1098 1098 Size: 4073 BanI G'GyrC_C Cuts at: 403 1089 1503 1751 1786 403 Size: 686 414 248 35 2690 Fragments arranged by size: 2690 686 414 248 35 BanII G_rGCy'C Cuts at: 137 1087 2117 137 Size: 950 1030 2093 Fragments arranged by size: 2093 1030 950 BbeI G_GCGC'C Cuts at: 1507 1755 1507 Size: 248 3825 BbsI GAAGACnn'nnnn_ Cuts at: 455 908 455 Size: 453 3620 BbvCI CC'TCA_GC Cuts at: 145 145 Size: 4073 BciVI GTATCCnnnnn_n' Cuts at: 1971 2732 4073 1971 Size: 761 1341 1971 Fragments arranged by size: 1971 1341 761 BclI T'GATC_A Cuts at: 1593 1593 Size: 4073 BglI GCCn_nnn'nGGC Cuts at: 974 974 Size: 4073 BglII A'GATC_T Cuts at: 1588 3625 1588 Size: 2037 2036 BlpI GC'TnA_GC Cuts at: 1185 1185 Size: 4073 Bme1580I G_kGCm'C Cuts at: 408 1698 1791 2847 408 Size: 1290 93 1056 1634 Fragments arranged by size: 1634 1290 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1285 1366 1701 3845 1285 Size: 81 335 2144 1513 Fragments arranged by size: 2144 1513 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2440 2440 Size: 4073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 252 363 252 Size: 111 3962 Bpu10I CC'TnA_GC Cuts at: 145 145 Size: 4073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1249 2128 2620 2918 1249 Size: 879 492 298 2404 Fragments arranged by size: 2404 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3777 3777 Size: 4073 BsaAI yAC'GTr Cuts at: 1426 1426 Size: 4073 BsaBI GATnn'nnATC Cuts at: 6 511 1587 3624 6 Size: 505 1076 2037 455 Fragments arranged by size: 2037 1076 505 455 BsaHI Gr'CG_yC Cuts at: 1504 1752 2454 3950 1504 Size: 248 702 1496 1627 Fragments arranged by size: 1627 1496 702 248 BsaWI w'CCGG_w Cuts at: 1262 1783 2461 2735 2882 3293 1262 Size: 521 678 274 147 411 2042 Fragments arranged by size: 2042 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 562 592 562 Size: 30 4043 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 213 319 213 Size: 106 3967 BseYI C'CCAG_C Cuts at: 1498 2256 2833 3329 1498 Size: 758 577 496 2242 Fragments arranged by size: 2242 758 577 496 BsiEI CG_ry'CG Cuts at: 104 759 1615 1661 2869 104 Size: 655 856 46 1208 1308 Fragments arranged by size: 1308 1208 856 655 46 BsiHKAI G_wGCw'C Cuts at: 1087 1865 2055 2847 1087 Size: 778 190 792 2313 Fragments arranged by size: 2313 792 778 190 BsmI GAATG_Cn' Cuts at: 330 330 Size: 4073 Bsp1286I G_dGCh'C Cuts at: 137 408 1087 1698 1791 1865 2055 2117 2847 Size: 271 679 611 93 74 190 62 730 Cuts at: 2847 137 Size: 1363 Fragments arranged by size: 1363 730 679 611 271 190 93 74 62 BspEI T'CCGG_A Cuts at: 3293 3293 Size: 4073 BspHI T'CATG_A Cuts at: 515 3249 515 Size: 2734 1339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1123 1337 1639 2020 1123 Size: 214 302 381 3176 Fragments arranged by size: 3176 381 302 214 BsrBI CCG'CTC Cuts at: 50 2365 2419 3903 4068 50 Size: 2315 54 1484 165 55 Fragments arranged by size: 2315 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 220 1985 220 Size: 1765 2308 BsrFI r'CCGG_y Cuts at: 2071 2252 2461 2071 Size: 181 209 3683 Fragments arranged by size: 3683 209 181 BssSI C'ACGA_G Cuts at: 2344 2702 2344 Size: 358 3715 BstAPI GCAn_nnn'nTGC Cuts at: 1450 1450 Size: 4073 BstBI TT'CG_AA Cuts at: 2434 2434 Size: 4073 BstYI r'GATC_y Cuts at: 1 249 1098 1565 1588 1923 2169 3181 3267 Size: 248 849 467 23 335 246 1012 86 Cuts at: 3267 3279 3625 1 Size: 12 346 449 Fragments arranged by size: 1012 849 467 449 346 335 248 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 196 196 Size: 4073 BtgI C'CryG_G Cuts at: 581 801 581 Size: 220 3853 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2023 2204 2367 2023 Size: 181 163 3729 Fragments arranged by size: 3729 181 163 BtsI GCAGTG_nn' Cuts at: 537 831 1059 1316 537 Size: 294 228 257 3294 Fragments arranged by size: 3294 294 257 228 DraI TTT'AAA Cuts at: 1072 1072 Size: 4073 DraIII CAC_nnn'GTG Cuts at: 342 342 Size: 4073 DrdI GACnn_nn'nnGTC Cuts at: 1779 2637 1779 Size: 858 3215 EaeI y'GGCC_r Cuts at: 621 1658 1832 2223 2250 3595 621 Size: 1037 174 391 27 1345 1099 Fragments arranged by size: 1345 1099 1037 391 174 27 EagI C'GGCC_G Cuts at: 1658 1658 Size: 4073 EarI CTCTTCn'nnn_ Cuts at: 160 345 2096 2306 160 Size: 185 1751 210 1927 Fragments arranged by size: 1927 1751 210 185 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2281 2591 2737 3883 2281 Size: 310 146 1146 2471 Fragments arranged by size: 2471 1146 310 146 EcoICRI GAG'CTC Cuts at: 1085 1085 Size: 4073 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 252 363 476 756 1898 2330 3077 3345 252 Size: 111 113 280 1142 432 747 268 980 Fragments arranged by size: 1142 980 747 432 280 268 113 111 EcoO109I rG'GnC_Cy Cuts at: 1212 1212 Size: 4073 EcoRI G'AATT_C Cuts at: 1077 1077 Size: 4073 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2092 2092 Size: 4073 FspI TGC'GCA Cuts at: 1854 1854 Size: 4073 HaeII r_GCGC'y Cuts at: 267 1507 1755 2777 3756 267 Size: 1240 248 1022 979 584 Fragments arranged by size: 1240 1022 979 584 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 562 594 1915 1945 1947 1977 562 Size: 32 1321 30 2 30 2658 Fragments arranged by size: 2658 1321 32 30 30 2 HincII GTy'rAC Cuts at: 1112 1112 Size: 4073 KasI G'GCGC_C Cuts at: 1503 1751 1503 Size: 248 3825 KpnI G_GTAC'C Cuts at: 1093 1093 Size: 4073 MluI A'CGCG_T Cuts at: 3506 3506 Size: 4073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2744 2928 2744 Size: 184 3889 MscI TGG'CCA Cuts at: 623 1834 623 Size: 1211 2862 MslI CAynn'nnrTG Cuts at: 629 2062 2189 629 Size: 1433 127 2513 Fragments arranged by size: 2513 1433 127 MspA1I CmG'CkG Cuts at: 144 1184 1498 1858 2871 3116 144 Size: 1040 314 360 1013 245 1101 Fragments arranged by size: 1101 1040 1013 360 314 245 NaeI GCC'GGC Cuts at: 2254 2254 Size: 4073 NarI GG'CG_CC Cuts at: 1504 1752 1504 Size: 248 3825 NdeI CA'TA_TG Cuts at: 291 291 Size: 4073 NgoMIV G'CCGG_C Cuts at: 2252 2252 Size: 4073 NspI r_CATG'y Cuts at: 1126 1626 2533 1126 Size: 500 907 2666 Fragments arranged by size: 2666 907 500 PciI A'CATG_T Cuts at: 2529 2529 Size: 4073 PmeI GTTT'AAAC Cuts at: 1072 1072 Size: 4073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3212 3244 3212 Size: 32 4041 PstI C_TGCA'G Cuts at: 1120 1805 1120 Size: 685 3388 PvuI CG_AT'CG Cuts at: 104 104 Size: 4073 PvuII CAG'CTG Cuts at: 144 1498 1858 144 Size: 1354 360 2359 Fragments arranged by size: 2359 1354 360 RsrII CG'GwC_CG Cuts at: 2268 2268 Size: 4073 SacI G_AGCT'C Cuts at: 1087 1087 Size: 4073 SalI G'TCGA_C Cuts at: 1110 1110 Size: 4073 SapI GCTCTTCn'nnn_ Cuts at: 160 2096 2306 160 Size: 1936 210 1927 Fragments arranged by size: 1936 1927 210 SbfI CC_TGCA'GG Cuts at: 1120 1120 Size: 4073 ScaI AGT'ACT Cuts at: 3590 3590 Size: 4073 SexAI A'CCwGG_T Cuts at: 399 399 Size: 4073 SfcI C'TryA_G Cuts at: 33 1116 1801 2794 2985 33 Size: 1083 685 993 191 1121 Fragments arranged by size: 1121 1083 993 685 191 SfoI GGC'GCC Cuts at: 1505 1753 1505 Size: 248 3825 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4073 SmaI CCC'GGG Cuts at: 1095 1095 Size: 4073 SmlI C'TyrA_G Cuts at: 1228 2143 2635 2897 1228 Size: 915 492 262 2404 Fragments arranged by size: 2404 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4073 SphI G_CATG'C Cuts at: 1126 1626 1126 Size: 500 3573 StyI C'CwwG_G Cuts at: 1011 1207 1561 1011 Size: 196 354 3523 Fragments arranged by size: 3523 354 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 539 745 1656 1789 2248 2457 539 Size: 206 911 133 459 209 2155 Fragments arranged by size: 2155 911 459 209 206 133 TatI w'GTAC_w Cuts at: 3588 3588 Size: 4073 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 341 373 3590 3622 341 Size: 32 3217 32 792 Fragments arranged by size: 3217 792 32 32 Tth111I GACn'n_nGTC Cuts at: 1870 1870 Size: 4073 XbaI T'CTAG_A Cuts at: 1104 1104 Size: 4073 XcmI CCAnnnn_n'nnnnTGG Cuts at: 616 1018 1568 616 Size: 402 550 3121 Fragments arranged by size: 3121 550 402 XmaI C'CCGG_G Cuts at: 1093 1093 Size: 4073 XmnI GAAnn'nnTTC Cuts at: 154 699 154 Size: 545 3528 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI