Restriction map for
pF1KB8111
(Circular) (Six-base) MAPSORT of: pF1KB8111.seq Check: 4500 from: 1 to: 4781
pF1KB8111 4781 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1257 1257
Size: 4781
AccI GT'mk_AC
Cuts at: 1819 1819
Size: 4781
Acc65I G'GTAC_C
Cuts at: 1797 1797
Size: 4781
AclI AA'CG_TT
Cuts at: 4621 4621
Size: 4781
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 3038 3785 4053 2606
Size: 432 747 268 3334
Fragments arranged by size:
3334 747 432 268
AflIII A'CryG_T
Cuts at: 2133 3237 4214 2133
Size: 1104 977 2700
Fragments arranged by size:
2700 1104 977
AgeI A'CCGG_T
Cuts at: 3169 3169
Size: 4781
AleI CACnn'nnGTG
Cuts at: 1707 1707
Size: 4781
AlwNI CAG_nnn'CTG
Cuts at: 2086 3653 2086
Size: 1567 3214
ApaI G_GGCC'C
Cuts at: 253 1492 253
Size: 1239 3542
ApaLI G'TGCA_C
Cuts at: 3551 3551
Size: 4781
ApoI r'AATT_y
Cuts at: 440 1018 1598 1635 1785 4134 4390 440
Size: 578 580 37 150 2349 256 831
Fragments arranged by size:
2349 831 580 578 256 150 37
AseI AT'TA_AT
Cuts at: 20 20
Size: 4781
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
AvaI C'yCGr_G
Cuts at: 404 1801 404
Size: 1397 3384
BamHI G'GATC_C
Cuts at: 1569 1806 1569
Size: 237 4544
BanI G'GyrC_C
Cuts at: 289 1451 1797 2211 2459 2494 289
Size: 1162 346 414 248 35 2576
Fragments arranged by size:
2576 1162 414 346 248 35
BanII G_rGCy'C
Cuts at: 253 909 1492 1795 2825 253
Size: 656 583 303 1030 2209
Fragments arranged by size:
2209 1030 656 583 303
BbeI G_GCGC'C
Cuts at: 293 2215 2463 293
Size: 1922 248 2611
Fragments arranged by size:
2611 1922 248
BbsI GAAGACnn'nnnn_
Cuts at: 1214 1265 1214
Size: 51 4730
BciVI GTATCCnnnnn_n'
Cuts at: 1096 2679 3440 4781 1096
Size: 1583 761 1341 1096
Fragments arranged by size:
1583 1341 1096 761
BclI T'GATC_A
Cuts at: 2301 2301
Size: 4781
BfrBI ATG'CAT
Cuts at: 917 917
Size: 4781
BglI GCCn_nnn'nGGC
Cuts at: 1256 1256
Size: 4781
BglII A'GATC_T
Cuts at: 2296 4333 2296
Size: 2037 2744
BlpI GC'TnA_GC
Cuts at: 1893 1893
Size: 4781
Bme1580I G_kGCm'C
Cuts at: 146 253 1492 2406 2499 3555 146
Size: 107 1239 914 93 1056 1372
Fragments arranged by size:
1372 1239 1056 914 107 93
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1993 2074 2409 4553 1486
Size: 507 81 335 2144 1714
Fragments arranged by size:
2144 1714 507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 4781
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 466 842 405
Size: 61 376 4344
Fragments arranged by size:
4344 376 61
Bpu10I CC'TnA_GC
Cuts at: 1246 1246
Size: 4781
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1957 2836 3328 3626 1957
Size: 879 492 298 3112
Fragments arranged by size:
3112 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 637 1031 4485 637
Size: 394 3454 933
Fragments arranged by size:
3454 933 394
BsaAI yAC'GTr
Cuts at: 1145 2134 1145
Size: 989 3792
BsaBI GATnn'nnATC
Cuts at: 6 2295 4332 6
Size: 2289 2037 455
Fragments arranged by size:
2289 2037 455
BsaHI Gr'CG_yC
Cuts at: 137 290 1168 1763 2212 2460 3162 4658 137
Size: 153 878 595 449 248 702 1496 260
Fragments arranged by size:
1496 878 702 595 449 260 248 153
BsaWI w'CCGG_w
Cuts at: 729 1970 2491 3169 3443 3590 4001 729
Size: 1241 521 678 274 147 411 1509
Fragments arranged by size:
1509 1241 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1357 1387 1357
Size: 30 4751
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1272 1275 1272
Size: 3 4778
BseYI C'CCAG_C
Cuts at: 311 1690 2206 2964 3541 4037 311
Size: 1379 516 758 577 496 1055
Fragments arranged by size:
1379 1055 758 577 516 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1141 1734 1792 1141
Size: 593 58 4130
Fragments arranged by size:
4130 593 58
BsiEI CG_ry'CG
Cuts at: 104 2323 2369 3577 104
Size: 2219 46 1208 1308
Fragments arranged by size:
2219 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1795 2573 2763 3555 1795
Size: 778 190 792 3021
Fragments arranged by size:
3021 792 778 190
BsmI GAATG_Cn'
Cuts at: 227 227
Size: 4781
Bsp1286I G_dGCh'C
Cuts at: 146 253 909 1492 1795 2406 2499 2573 2763
Size: 107 656 583 303 611 93 74 190
Cuts at: 2763 2825 3555 146
Size: 62 730 1372
Fragments arranged by size:
1372 730 656 611 583 303 190 107
93 74 62
BspEI T'CCGG_A
Cuts at: 4001 4001
Size: 4781
BspHI T'CATG_A
Cuts at: 3957 3957
Size: 4781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1163 1199 1257 1764 1831 2045 2347 2728 1163
Size: 36 58 507 67 214 302 381 3216
Fragments arranged by size:
3216 507 381 302 214 67 58 36
BsrBI CCG'CTC
Cuts at: 50 498 3073 3127 4611 4776 50
Size: 448 2575 54 1484 165 55
Fragments arranged by size:
2575 1484 448 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2693 2693
Size: 4781
BsrFI r'CCGG_y
Cuts at: 292 1194 2779 2960 3169 292
Size: 902 1585 181 209 1904
Fragments arranged by size:
1904 1585 902 209 181
BssSI C'ACGA_G
Cuts at: 1042 3052 3410 1042
Size: 2010 358 2413
Fragments arranged by size:
2413 2010 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2158 2158
Size: 4781
BstBI TT'CG_AA
Cuts at: 3142 3142
Size: 4781
BstYI r'GATC_y
Cuts at: 1 1569 1721 1806 2273 2296 2631 2877 3889
Size: 1568 152 85 467 23 335 246 1012
Cuts at: 3889 3975 3987 4333 1
Size: 86 12 346 449
Fragments arranged by size:
1568 1012 467 449 346 335 246 152
86 85 23 12
Bsu36I CC'TnA_GG
Cuts at: 344 1028 344
Size: 684 4097
BtgI C'CryG_G
Cuts at: 107 257 389 107
Size: 150 132 4499
Fragments arranged by size:
4499 150 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2731 2912 3075 2731
Size: 181 163 4437
Fragments arranged by size:
4437 181 163
BtsI GCAGTG_nn'
Cuts at: 266 2024 266
Size: 1758 3023
DraI TTT'AAA
Cuts at: 1641 1780 1641
Size: 139 4642
DrdI GACnn_nn'nnGTC
Cuts at: 2487 3345 2487
Size: 858 3923
EaeI y'GGCC_r
Cuts at: 110 2366 2540 2931 2958 4303 110
Size: 2256 174 391 27 1345 588
Fragments arranged by size:
2256 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 2366 2366
Size: 4781
EarI CTCTTCn'nnn_
Cuts at: 1405 2804 3014 1405
Size: 1399 210 3172
Fragments arranged by size:
3172 1399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 308 870 1454 2989 3299 3445 4591 308
Size: 562 584 1535 310 146 1146 498
Fragments arranged by size:
1535 1146 584 562 498 310 146
EcoICRI GAG'CTC
Cuts at: 1793 1793
Size: 4781
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 466 842 2606 3038 3785 4053 405
Size: 61 376 1764 432 747 268 1133
Fragments arranged by size:
1764 1133 747 432 376 268 61
EcoO109I rG'GnC_Cy
Cuts at: 197 249 250 477 720 1920 197
Size: 52 1 227 243 1200 3058
Fragments arranged by size:
3058 1200 243 227 52 1
EcoRI G'AATT_C
Cuts at: 1785 1785
Size: 4781
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 561 2800 561
Size: 2239 2542
FspI TGC'GCA
Cuts at: 2562 2562
Size: 4781
HaeII r_GCGC'y
Cuts at: 293 2215 2463 3485 4464 293
Size: 1922 248 1022 979 610
Fragments arranged by size:
1922 1022 979 610 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 493 525 661 693 1351 1357 1383 1389 1662
Size: 32 136 32 658 6 26 6 273
Cuts at: 1662 1694 2623 2653 2655 2685 493
Size: 32 929 30 2 30 2589
Fragments arranged by size:
2589 929 658 273 136 32 32 32
30 30 26 6 6 2
HincII GTy'rAC
Cuts at: 1578 1820 1578
Size: 242 4539
HindIII A'AGCT_T
Cuts at: 538 538
Size: 4781
KasI G'GCGC_C
Cuts at: 289 2211 2459 289
Size: 1922 248 2611
Fragments arranged by size:
2611 1922 248
KpnI G_GTAC'C
Cuts at: 1801 1801
Size: 4781
MluI A'CGCG_T
Cuts at: 4214 4214
Size: 4781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 977 1184 1331 1403 3452 3636 977
Size: 207 147 72 2049 184 2122
Fragments arranged by size:
2122 2049 207 184 147 72
MscI TGG'CCA
Cuts at: 112 2542 112
Size: 2430 2351
MslI CAynn'nnrTG
Cuts at: 1267 1707 2770 2897 1267
Size: 440 1063 127 3151
Fragments arranged by size:
3151 1063 440 127
MspA1I CmG'CkG
Cuts at: 129 1533 1694 1768 1892 2206 2566 3579 3824
Size: 1404 161 74 124 314 360 1013 245
Cuts at: 3824 129
Size: 1086
Fragments arranged by size:
1404 1086 1013 360 314 245 161 124
74
NaeI GCC'GGC
Cuts at: 294 1196 2962 294
Size: 902 1766 2113
Fragments arranged by size:
2113 1766 902
NarI GG'CG_CC
Cuts at: 290 2212 2460 290
Size: 1922 248 2611
Fragments arranged by size:
2611 1922 248
NcoI C'CATG_G
Cuts at: 107 257 107
Size: 150 4631
NgoMIV G'CCGG_C
Cuts at: 292 1194 2960 292
Size: 902 1766 2113
Fragments arranged by size:
2113 1766 902
NsiI A_TGCA'T
Cuts at: 919 919
Size: 4781
NspI r_CATG'y
Cuts at: 310 1266 1834 2334 3241 310
Size: 956 568 500 907 1850
Fragments arranged by size:
1850 956 907 568 500
PciI A'CATG_T
Cuts at: 3237 3237
Size: 4781
PflMI CCAn_nnn'nTGG
Cuts at: 933 933
Size: 4781
PfoI T'CCnGG_A
Cuts at: 1724 1724
Size: 4781
PmeI GTTT'AAAC
Cuts at: 1780 1780
Size: 4781
PmlI CAC'GTG
Cuts at: 1145 1145
Size: 4781
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3920 3952 3920
Size: 32 4749
PpuMI rG'GwC_Cy
Cuts at: 197 477 720 197
Size: 280 243 4258
Fragments arranged by size:
4258 280 243
PspOMI G'GGCC_C
Cuts at: 249 1488 249
Size: 1239 3542
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1373 1405 1373
Size: 32 4749
PstI C_TGCA'G
Cuts at: 273 305 1828 2513 273
Size: 32 1523 685 2541
Fragments arranged by size:
2541 1523 685 32
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4781
PvuII CAG'CTG
Cuts at: 129 1533 2206 2566 129
Size: 1404 673 360 2344
Fragments arranged by size:
2344 1404 673 360
RsrII CG'GwC_CG
Cuts at: 2976 2976
Size: 4781
SacI G_AGCT'C
Cuts at: 1795 1795
Size: 4781
SalI G'TCGA_C
Cuts at: 1818 1818
Size: 4781
SapI GCTCTTCn'nnn_
Cuts at: 2804 3014 2804
Size: 210 4571
SbfI CC_TGCA'GG
Cuts at: 1828 1828
Size: 4781
ScaI AGT'ACT
Cuts at: 691 4298 691
Size: 3607 1174
SfcI C'TryA_G
Cuts at: 33 269 301 1824 2509 3502 3693 33
Size: 236 32 1523 685 993 191 1121
Fragments arranged by size:
1523 1121 993 685 236 191 32
SfoI GGC'GCC
Cuts at: 291 2213 2461 291
Size: 1922 248 2611
Fragments arranged by size:
2611 1922 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4781
SmaI CCC'GGG
Cuts at: 1803 1803
Size: 4781
SmlI C'TyrA_G
Cuts at: 404 1936 2851 3343 3605 404
Size: 1532 915 492 262 1580
Fragments arranged by size:
1580 1532 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4781
SphI G_CATG'C
Cuts at: 310 1266 1834 2334 310
Size: 956 568 500 2757
Fragments arranged by size:
2757 956 568 500
StyI C'CwwG_G
Cuts at: 107 257 369 1447 1603 1915 2269 107
Size: 150 112 1078 156 312 354 2619
Fragments arranged by size:
2619 1078 354 312 156 150 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2364 2497 2956 3165 2364
Size: 133 459 209 3980
Fragments arranged by size:
3980 459 209 133
TatI w'GTAC_w
Cuts at: 689 1349 4296 689
Size: 660 2947 1174
Fragments arranged by size:
2947 1174 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4298 4330 4298
Size: 32 4749
Tth111I GACn'n_nGTC
Cuts at: 207 2578 207
Size: 2371 2410
XbaI T'CTAG_A
Cuts at: 1812 1812
Size: 4781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 396 831 2276 396
Size: 435 1445 2901
Fragments arranged by size:
2901 1445 435
XhoI C'TCGA_G
Cuts at: 404 404
Size: 4781
XmaI C'CCGG_G
Cuts at: 1801 1801
Size: 4781
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI
NruI PacI PasI PshAI PsiI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XmnI ZraI