Restriction map for pF1KB8110
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       BalI        BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfrI        
BglII       BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TaqI        TfiI        
Tru9I       Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AlwNI         135  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          506  GGGCCC               GGGCC^C
AvrII         174  CCTAGG               C^CTAGG
BamHI         424  GGATCC               G^GATCC
BbvCI         532  GCTGAGG              CCTCAGC(-5/-2)
BglI          131  GCCACAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          174  CCTAGG               C^CTAGG
BlpI          390  GCTGAGC              GC^T[ACGT]AGC
BpmI          286  CTGGAG               CTGGAG(16/14)
Bpu1102I      390  GCTGAGC              GC^T[ACGT]AGC
BseRI         304  GAGGAG               GAGGAG(10/8)
BstXI          35  CCATGCTACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         390  GCTGAGC              GC^T[ACGT]AGC
EarI          523  GAAGAG               CTCTTC(1/4)
EcoT14I       174  CCTAGG               C^C[AT][AT]GG
FauI          473  GCGGG                CCCGC(4/6)
Ksp632I       523  GAAGAG               CTCTTC(1/4)
MspA1I        135  CAGCGG               C[AC]G^C[GT]G
NspI          251  GCATGC               [GA]CATG^[CT]
PflMI         120  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI          294  CCCGGG               CCC^GGG
SphI          251  GCATGC               GCATG^C
StuI          359  AGGCCT               AGG^CCT
StyI          174  CCTAGG               C^C[AT][AT]GG
Van91I        120  CCAGATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         294  CCCGGG               C^CCGGG
XmaI          294  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AvaI          294  CCCGGG               C^[CT]CG[GA]G
              490  CCCGAG              
AvaII           9  GGTCC                G^G[AT]CC
              495  GGACC               
BanI          309  GGTGCC               G^G[CT][GA]CC
              436  GGCACC              
BanII         487  GAGCCC               G[GA]GC[CT]^C
              506  GGGCCC              
BbvI           46  GCTGC                GCAGC(8/12)
              353  GCTGC               
BfaI          175  CTAG                 C^TAG
              377  CTAG                
BsgI          343  CTGCAC               GTGCAG(16/14)
              403  GTGCAG              
BsmFI         199  GTCCC                GGGAC(10/14)
              228  GTCCC               
BsoBI         294  CCCGGG               C^[CT]CG[GA]G
              490  CCCGAG              
BsrI           42  ACTGG                ACTGG(1/-1)
              261  CCAGT               
BssSI         301  CACGAG               CACGAG(-5/-1)
              382  CACGAG              
BstYI         338  AGATCC               [GA]^GATC[CT]
              424  GGATCC              
HinfI         226  GAGTC                G^A[ACGT]TC
              244  GACTC               
HphI          116  TCACC                GGTGA(8/7)
              447  TCACC               
MaeI          175  CTAG                 C^TAG
              377  CTAG                
MboII         336  GAAGA                GAAGA(8/7)
              523  GAAGA               
MflI          338  AGATCC               [GA]^GATC[CT]
              424  GGATCC              
MwoI          131  GCCACAGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              356  GCCAGGCCTGC         
PleI          244  GACTC                GAGTC(4/5)
              226  GAGTC               
PpuMI           8  GGGTCCT              [GA]G^G[AT]CC[CT]
              494  AGGACCC             
SfcI          192  CTACAG               C^T[GA][CT]AG
              348  CTGTAG              
TseI           46  GCTGC                G^C[AT]GC
              353  GCTGC               
XhoII         338  AGATCC               [GA]^GATC[CT]
              424  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AciI        AluI        AlwI        BcnI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       DraII       EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HgaI        HpaII       
ItaI        MaeIII      MboI        MnlI        MspI        
MvaI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       Tsp45I      TspRI       
XcmI        
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