Restriction map for pF1KB8109
 (Circular) (Six-base) MAPSORT of: pF1KB8109.seq  Check: 9851  from: 1  to: 3572



pF1KB8109  3572 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    335     610     335 
   Size:        275    3297
 
Acc65I G'GTAC_C

Cuts at:    588     588 
   Size:       3572
 
AclI AA'CG_TT

Cuts at:   3412    3412 
   Size:       3572
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1397    1829    2576    2844     530 
   Size:        867     432     747     268    1258

  Fragments arranged by size: 

                1258     867     747     432     268
 
AflIII A'CryG_T

Cuts at:    924    2028    3005     924 
   Size:       1104     977    1491

  Fragments arranged by size: 

                1491    1104     977
 
AgeI A'CCGG_T

Cuts at:   1960    1960 
   Size:       3572
 
AlwNI CAG_nnn'CTG

Cuts at:    877    2444     877 
   Size:       1567    2005
 
ApaLI G'TGCA_C

Cuts at:   2342    2342 
   Size:       3572
 
ApoI r'AATT_y

Cuts at:    576    2925    3181     576 
   Size:       2349     256     967

  Fragments arranged by size: 

                2349     967     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3572
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3572
 
AvaI C'yCGr_G

Cuts at:    248     592     248 
   Size:        344    3228
 
BamHI G'GATC_C

Cuts at:    597     597 
   Size:       3572
 
BanI G'GyrC_C

Cuts at:    534     588    1002    1250    1285     534 
   Size:         54     414     248      35    2821

  Fragments arranged by size: 

                2821     414     248      54      35
 
BanII G_rGCy'C

Cuts at:    586    1616     586 
   Size:       1030    2542
 
BbeI G_GCGC'C

Cuts at:   1006    1254    1006 
   Size:        248    3324
 
BbsI GAAGACnn'nnnn_

Cuts at:    184     195     184 
   Size:         11    3561
 
BciVI GTATCCnnnnn_n'

Cuts at:   1470    2231    3572    1470 
   Size:        761    1341    1470

  Fragments arranged by size: 

                1470    1341     761
 
BclI T'GATC_A

Cuts at:   1092    1092 
   Size:       3572
 
BglII A'GATC_T

Cuts at:    278    1087    3124     278 
   Size:        809    2037     726

  Fragments arranged by size: 

                2037     809     726
 
BlpI GC'TnA_GC

Cuts at:    684     684 
   Size:       3572
 
Bme1580I G_kGCm'C

Cuts at:    361     537    1197    1290    2346     361 
   Size:        176     660      93    1056    1587

  Fragments arranged by size: 

                1587    1056     660     176      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    784     865    1200    3344     784 
   Size:         81     335    2144    1012

  Fragments arranged by size: 

                2144    1012     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1939    1939 
   Size:       3572
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    748    1627    2119    2417     748 
   Size:        879     492     298    1903

  Fragments arranged by size: 

                1903     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    552    3276     552 
   Size:       2724     848
 
BsaAI yAC'GTr

Cuts at:    925     925 
   Size:       3572
 
BsaBI GATnn'nnATC

Cuts at:      6    1086    3123       6 
   Size:       1080    2037     455

  Fragments arranged by size: 

                2037    1080     455
 
BsaHI Gr'CG_yC

Cuts at:   1003    1251    1953    3449    1003 
   Size:        248     702    1496    1126

  Fragments arranged by size: 

                1496    1126     702     248
 
BsaWI w'CCGG_w

Cuts at:    761    1282    1960    2234    2381    2792     761 
   Size:        521     678     274     147     411    1541

  Fragments arranged by size: 

                1541     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    229     259     229 
   Size:         30    3542
 
BseYI C'CCAG_C

Cuts at:    997    1755    2332    2828     997 
   Size:        758     577     496    1741

  Fragments arranged by size: 

                1741     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     167     171     222    1114    1160    2368     104 
   Size:         63       4      51     892      46    1208    1308

  Fragments arranged by size: 

                1308    1208     892      63      51      46       4
 
BsiHKAI G_wGCw'C

Cuts at:    586    1364    1554    2346     586 
   Size:        778     190     792    1812

  Fragments arranged by size: 

                1812     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    361     537     586    1197    1290    1364    1554    1616    2346 
   Size:        176      49     611      93      74     190      62     730

Cuts at:   2346     361 
   Size:       1587

  Fragments arranged by size: 

                1587     730     611     190     176      93      74      62
                  49
 
BspEI T'CCGG_A

Cuts at:   2792    2792 
   Size:       3572
 
BspHI T'CATG_A

Cuts at:   2748    2748 
   Size:       3572
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    622     836    1138    1519     622 
   Size:        214     302     381    2675

  Fragments arranged by size: 

                2675     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1864    1918    3402    3567      50 
   Size:       1814      54    1484     165      55

  Fragments arranged by size: 

                1814    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1484    1484 
   Size:       3572
 
BsrFI r'CCGG_y

Cuts at:   1570    1751    1960    1570 
   Size:        181     209    3182

  Fragments arranged by size: 

                3182     209     181
 
BssSI C'ACGA_G

Cuts at:   1843    2201    1843 
   Size:        358    3214
 
BstAPI GCAn_nnn'nTGC

Cuts at:    949     949 
   Size:       3572
 
BstBI TT'CG_AA

Cuts at:   1933    1933 
   Size:       3572
 
BstXI CCAn_nnnn'nTGG

Cuts at:    114     114 
   Size:       3572
 
BstYI r'GATC_y

Cuts at:      1     278     597    1064    1087    1422    1668    2680    2766 
   Size:        277     319     467      23     335     246    1012      86

Cuts at:   2766    2778    3124       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     319     277     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3572
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1522    1703    1866    1522 
   Size:        181     163    3228

  Fragments arranged by size: 

                3228     181     163
 
BtsI GCAGTG_nn'

Cuts at:    815     815 
   Size:       3572
 
DraI TTT'AAA

Cuts at:    571     571 
   Size:       3572
 
DrdI GACnn_nn'nnGTC

Cuts at:    474    1278    2136     474 
   Size:        804     858    1910

  Fragments arranged by size: 

                1910     858     804
 
EaeI y'GGCC_r

Cuts at:    219    1157    1331    1722    1749    3094     219 
   Size:        938     174     391      27    1345     697

  Fragments arranged by size: 

                1345     938     697     391     174      27
 
EagI C'GGCC_G

Cuts at:    219    1157     219 
   Size:        938    2634
 
EarI CTCTTCn'nnn_

Cuts at:   1595    1805    1595 
   Size:        210    3362
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1780    2090    2236    3382    1780 
   Size:        310     146    1146    1970

  Fragments arranged by size: 

                1970    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    584     584 
   Size:       3572
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    530    1397    1829    2576    2844     530 
   Size:        867     432     747     268    1258

  Fragments arranged by size: 

                1258     867     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    545     545 
   Size:       3572
 
EcoO109I rG'GnC_Cy

Cuts at:    711     711 
   Size:       3572
 
EcoRI G'AATT_C

Cuts at:    576     576 
   Size:       3572
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1591    1591 
   Size:       3572
 
FspI TGC'GCA

Cuts at:   1353    1353 
   Size:       3572
 
HaeII r_GCGC'y

Cuts at:   1006    1254    2276    3255    1006 
   Size:        248    1022     979    1323

  Fragments arranged by size: 

                1323    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    229     261    1414    1444    1446    1476     229 
   Size:         32    1153      30       2      30    2325

  Fragments arranged by size: 

                2325    1153      32      30      30       2
 
HincII GTy'rAC

Cuts at:    611     611 
   Size:       3572
 
HindIII A'AGCT_T

Cuts at:    318     318 
   Size:       3572
 
KasI G'GCGC_C

Cuts at:   1002    1250    1002 
   Size:        248    3324
 
KpnI G_GTAC'C

Cuts at:    592     592 
   Size:       3572
 
MluI A'CGCG_T

Cuts at:   3005    3005 
   Size:       3572
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2243    2427    2243 
   Size:        184    3388
 
MscI TGG'CCA

Cuts at:   1333    1333 
   Size:       3572
 
MslI CAynn'nnrTG

Cuts at:    112     289    1561    1688     112 
   Size:        177    1272     127    1996

  Fragments arranged by size: 

                1996    1272     177     127
 
MspA1I CmG'CkG

Cuts at:    683     997    1357    2370    2615     683 
   Size:        314     360    1013     245    1640

  Fragments arranged by size: 

                1640    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1753    1753 
   Size:       3572
 
NarI GG'CG_CC

Cuts at:   1003    1251    1003 
   Size:        248    3324
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3572
 
NdeI CA'TA_TG

Cuts at:    564     564 
   Size:       3572
 
NgoMIV G'CCGG_C

Cuts at:   1751    1751 
   Size:       3572
 
NspI r_CATG'y

Cuts at:    185     625    1125    2032     185 
   Size:        440     500     907    1725

  Fragments arranged by size: 

                1725     907     500     440
 
PciI A'CATG_T

Cuts at:   2028    2028 
   Size:       3572
 
PflMI CCAn_nnn'nTGG

Cuts at:    234     234 
   Size:       3572
 
PmeI GTTT'AAAC

Cuts at:    571     571 
   Size:       3572
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2711    2743    2711 
   Size:         32    3540
 
PstI C_TGCA'G

Cuts at:    476     619    1304     476 
   Size:        143     685    2744

  Fragments arranged by size: 

                2744     685     143
 
PvuI CG_AT'CG

Cuts at:    104     167     104 
   Size:         63    3509
 
PvuII CAG'CTG

Cuts at:    997    1357     997 
   Size:        360    3212
 
RsrII CG'GwC_CG

Cuts at:   1767    1767 
   Size:       3572
 
SacI G_AGCT'C

Cuts at:    586     586 
   Size:       3572
 
SalI G'TCGA_C

Cuts at:    609     609 
   Size:       3572
 
SapI GCTCTTCn'nnn_

Cuts at:   1595    1805    1595 
   Size:        210    3362
 
SbfI CC_TGCA'GG

Cuts at:    619     619 
   Size:       3572
 
ScaI AGT'ACT

Cuts at:   3089    3089 
   Size:       3572
 
SfcI C'TryA_G

Cuts at:     33     472     615    1300    2293    2484      33 
   Size:        439     143     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     439     191     143
 
SfoI GGC'GCC

Cuts at:   1004    1252    1004 
   Size:        248    3324
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3572
 
SmaI CCC'GGG

Cuts at:    250     594     250 
   Size:        344    3228
 
SmlI C'TyrA_G

Cuts at:    727    1642    2134    2396     727 
   Size:        915     492     262    1903

  Fragments arranged by size: 

                1903     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3572
 
SphI G_CATG'C

Cuts at:    625    1125     625 
   Size:        500    3072
 
StyI C'CwwG_G

Cuts at:    107     706    1060     107 
   Size:        599     354    2619

  Fragments arranged by size: 

                2619     599     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1155    1288    1747    1956    1155 
   Size:        133     459     209    2771

  Fragments arranged by size: 

                2771     459     209     133
 
TatI w'GTAC_w

Cuts at:   3087    3087 
   Size:       3572
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3089    3121    3089 
   Size:         32    3540
 
Tth111I GACn'n_nGTC

Cuts at:   1369    1369 
   Size:       3572
 
XbaI T'CTAG_A

Cuts at:    603     603 
   Size:       3572
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1067    1067 
   Size:       3572
 
XmaI C'CCGG_G

Cuts at:    248     592     248 
   Size:        344    3228
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI     PciI
    PflMI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI   Bpu10I    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoRV     FseI    FspAI     HpaI     MfeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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