Restriction map for pF1KB8107
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MflI        MluI        
MroI        MseI        MspA1I      MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AccII         285  CGCG                 CG^CG
AcsI          500  GAATTC               [GA]^AATT[CT]
AlwI          413  GGATC                GGATC(4/5)
ApoI          500  GAATTC               [GA]^AATT[CT]
BclI          200  TGATCA               T^GATCA
BcnI          644  CCCGG                CC^[GC]GG
BsmFI         642  GTCCC                GGGAC(10/14)
BstUI         285  CGCG                 CG^CG
BstXI          35  CCAAAGAACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII         523  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      523  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         500  GAATTC               G^AATTC
EcoRV         106  GATATC               GAT^ATC
EcoT22I       233  ATGCAT               ATGCA^T
FbaI          200  TGATCA               T^GATCA
HapII         645  CCGG                 C^CGG
HincII         88  GTTGAC               GT[CT]^[GA]AC
HindII         88  GTTGAC               GT[CT]^[GA]AC
HpaII         645  CCGG                 C^CGG
MaeIII        550  GTGAC                ^GT[ACGT]AC
MspI          645  CCGG                 C^CGG
MvnI          285  CGCG                 CG^CG
NciI          644  CCCGG                CC^[GC]GG
NsiI          233  ATGCAT               ATGCA^T
PstI          310  CTGCAG               CTGCA^G
SapI          315  GAAGAGC              GCTCTTC(1/4)
SfcI          310  CTGCAG               C^T[GA][CT]AG
Tsp45I        550  GTGAC                ^GT[GC]AC
TspRI         257  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AluI          227  AGCT                 AG^CT
              281  AGCT                
AspHI         259  GTGCTC               G[AT]GC[AT]^C
              318  GAGCAC              
BalI          188  TGGCCA               TGG^CCA
              650  TGGCCA              
BmyI          259  GTGCTC               G[AGT]GC[ACT]^C
              318  GAGCAC              
BpmI          578  CTCCAG               CTGGAG(16/14)
              628  CTGGAG              
BsiHKAI       259  GTGCTC               G[AT]GC[AT]^C
              318  GAGCAC              
BsiYI         279  CCAGCTCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              374  CCACCATGAGG         
BslI          279  CCAGCTCGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              374  CCACCATGAGG         
Bsp1286I      259  GTGCTC               G[AGT]GC[ACT]^C
              318  GAGCAC              
BsrI           42  ACTGG                ACTGG(1/-1)
              542  CCAGT               
BstNI          21  CCAGG                CC^[AT]GG
              580  CCAGG               
Cfr13I        162  GGGCC                G^G[ACGT]CC
              524  GGGCC               
DdeI          255  CTCAG                C^T[ACGT]AG
              597  CTCAG               
EaeI          188  TGGCCA               [CT]^GGCC[GA]
              650  TGGCCA              
EarI          315  GAAGAG               CTCTTC(1/4)
              619  GAAGAG              
EcoRII         21  CCAGG                ^CC[AT]GG
              580  CCAGG               
EcoT14I       191  CCAAGG               C^C[AT][AT]GG
              527  CCTTGG              
HinfI         357  GATTC                G^A[ACGT]TC
              366  GATTC               
Ksp632I       315  GAAGAG               CTCTTC(1/4)
              619  GAAGAG              
MluNI         188  TGGCCA               TGG^CCA
              650  TGGCCA              
MscI          188  TGGCCA               TGG^CCA
              650  TGGCCA              
MslI          264  CATGGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              321  CACAGAAGTG          
MvaI           21  CCAGG                CC^[AT]GG
              580  CCAGG               
NlaIV         304  GGTTCC               GG[ACGT]^[ACGT]CC
              523  GGGGCC              
Sau96I        162  GGGCC                G^G[ACGT]CC
              524  GGGCC               
StyI          191  CCAAGG               C^C[AT][AT]GG
              527  CCTTGG              
TfiI          357  GATTC                G^A[AT]TC
              366  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        BbvI        BsaJI       BseRI       BssKI       
BstF5I      Cac8I       DpnII       Fnu4HI      FokI        
HaeIII      ItaI        MboI        MboII       MnlI        
MwoI        NdeII       NlaIII      Sau3AI      ScrFI       
SfaNI       TseI        Tsp509I     
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