Restriction map for pF1KB8104
##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AgeI        AhdI        AocI        ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbsI        BclI        BfrI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsiWI       
BsmBI       BsmI        BspDI       BspHI       BsrDI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        Eam1105I    Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        MamI        MfeI        MluI        MslI        
MunI        NarI        NdeI        NheI        NruI        
NsiI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SalI        
SbfI        ScaI        SexAI       SfoI        SfuI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tsp509I     Tth111I     
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccIII        509  TCCGGA               T^CCGGA
AcyI          796  GGCGTC               G[GA]^CG[CT]C
AfaI          864  GTAC                 GT^AC
AflIII        860  ACATGT               A^C[GA][CT]GT
Alw44I       1207  GTGCAC               G^TGCAC
ApaLI        1207  GTGCAC               G^TGCAC
BamHI         885  GGATCC               G^GATCC
BanI          604  GGCACC               G^G[CT][GA]CC
BciVI        1156  GTATCC               GTATCC(6/5)
BfaI          811  CTAG                 C^TAG
BlpI          423  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      423  GCTGAGC              GC^T[ACGT]AGC
BsaHI         796  GGCGTC               G[GA]^CG[CT]C
BsaI          372  GGTCTC               GGTCTC(1/5)
BsaWI         509  TCCGGA               [AT]^CCGG[AT]
BseAI         509  TCCGGA               T^CCGGA
BsmAI         373  GTCTC                GTCTC(1/5)
Bsp1407I      863  TGTACA               T^GTACA
BspEI         509  TCCGGA               T^CCGGA
BspLU11I      860  ACATGT               A^CATGT
BsrGI         863  TGTACA               T^GTACA
BssSI        1177  CACGAG               CACGAG(-5/-1)
BstAPI        744  GCAGGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1359  CATCC                GGATG(2/0)
CelII         423  GCTGAGC              GC^T[ACGT]AGC
EcoNI         810  CCTAGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI         1359  CATCC                GGATG(9/13)
Hin1I         796  GGCGTC               G[GA]^CG[CT]C
HinfI         406  GACTC                G^A[ACGT]TC
MaeI          811  CTAG                 C^TAG
MroI          509  TCCGGA               T^CCGGA
MseI          593  TTAA                 T^TAA
NaeI         1228  GCCGGC               GCC^GGC
NcoI          515  CCATGG               C^CATGG
NgoMIV       1228  GCCGGC               G^CCGGC
NotI          139  GCGGCCGC             GC^GGCCGC
PaeR7I        400  CTCGAG               C^TCGAG
PleI          406  GACTC                GAGTC(4/5)
RsaI          864  GTAC                 GT^AC
SfiI          165  GGCCCGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          400  CTCGAG               C^T[CT][GA]AG
SspBI         863  TGTACA               T^GTACA
Tru9I         593  TTAA                 T^TAA
XhoI          400  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AlwNI         745  CAGGACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1279  CAGATCCTG           
BalI         1275  TGGCCA               TGG^CCA
             1364  TGGCCA              
BbvCI         238  CCTCAGC              CCTCAGC(-5/-2)
             1107  GCTGAGG             
BcgI          974  GCACCACCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1059  GCAGGTGCTTCG        
BsgI          457  GTGCAG               GTGCAG(16/14)
              565  GTGCAG              
BssHII        118  GCGCGC               G^CGCGC
              921  GCGCGC              
DraIII        703  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1177  CACGAGGTG           
EagI           34  CGGCCG               C^GGCCG
              140  CGGCCG              
EclXI          34  CGGCCG               C^GGCCG
              140  CGGCCG              
Eco52I         34  CGGCCG               C^GGCCG
              140  CGGCCG              
EcoT14I       515  CCATGG               C^C[AT][AT]GG
              827  CCAAGG              
MaeIII        250  GTGAC                ^GT[ACGT]AC
              928  GTCAC               
MluNI        1275  TGGCCA               TGG^CCA
             1364  TGGCCA              
MscI         1275  TGGCCA               TGG^CCA
             1364  TGGCCA              
NspI          860  ACATGT               [GA]CATG^[CT]
              944  ACATGC              
PflMI         702  CCACGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1278  CCAGATCCTGG         
PpuMI         194  AGGTCCC              [GA]G^G[AT]CC[CT]
              746  AGGACCT             
PshAI         478  GACGTGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
              513  GACCATGGTC          
SapI          108  GCTCTTC              GCTCTTC(1/4)
              329  GCTCTTC             
SgrAI        1227  CGCCGGCG             C[GA]^CCGG[CT]G
             1291  CACCGGCG            
StyI          515  CCATGG               C^C[AT][AT]GG
              827  CCAAGG              
Tsp45I        250  GTGAC                ^GT[GC]AC
              928  GTCAC               
Van91I        702  CCACGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1278  CCAGATCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        Aor51HI     
ApaI        AspHI       AvaI        AvaII       BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco47III    EcoO109I    EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     KspI        MaeII       MboI        
MboII       MflI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        PvuII       SacII       
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        TaqI        TseI        TspRI       TthHB8I     
XhoII       XmaCI       XmaI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com