Restriction map for pF1KB8104
 (Linear) (Six-base) MAPSORT of: KIBB8104.seq  Check: 9183  from: 1  to: 1383



KIBB8104.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     347    1050    1383 
   Size:        347     703     333

  Fragments arranged by size: 

                 703     347     333
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     643     911    1152    1383 
   Size:        643     268     241     231

  Fragments arranged by size: 

                 643     268     241     231
 
AfeI AGC'GCT

Cuts at:      0     553     628     646    1383 
   Size:        553      75      18     737

  Fragments arranged by size: 

                 737     553      75      18
 
AflIII A'CryG_T

Cuts at:      0     860    1383 
   Size:        860     523
 
AlwNI CAG_nnn'CTG

Cuts at:      0     750    1284    1383 
   Size:        750     534      99

  Fragments arranged by size: 

                 750     534      99
 
ApaI G_GGCC'C

Cuts at:      0      19      78     168    1383 
   Size:         19      59      90    1215

  Fragments arranged by size: 

                1215      90      59      19
 
ApaLI G'TGCA_C

Cuts at:      0    1207    1383 
   Size:       1207     176
 
AvaI C'yCGr_G

Cuts at:      0      10      60     161     400    1383 
   Size:         10      50     101     239     983

  Fragments arranged by size: 

                 983     239     101      50      10
 
BamHI G'GATC_C

Cuts at:      0     885    1383 
   Size:        885     498
 
BanI G'GyrC_C

Cuts at:      0     604    1383 
   Size:        604     779
 
BanII G_rGCy'C

Cuts at:      0      19      78     161     168     219     266     331    1383 
   Size:         19      59      83       7      51      47      65    1052

  Fragments arranged by size: 

                1052      83      65      59      51      47      19       7
            
 
BbvCI CC'TCA_GC

Cuts at:      0     239    1108    1383 
   Size:        239     869     275

  Fragments arranged by size: 

                 869     275     239
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     963     997    1048    1082    1383 
   Size:        963      34      51      34     301

  Fragments arranged by size: 

                 963     301      51      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1167    1383 
   Size:       1167     216
 
BglI GCCn_nnn'nGGC

Cuts at:      0      23     172    1372    1383 
   Size:         23     149    1200      11

  Fragments arranged by size: 

                1200     149      23      11
 
BlpI GC'TnA_GC

Cuts at:      0     424    1383 
   Size:        424     959
 
Bme1580I G_kGCm'C

Cuts at:      0      19      78     168     275     581     607    1211    1383 
   Size:         19      59      90     107     306      26     604     172

  Fragments arranged by size: 

                 604     306     172     107      90      59      26      19
            
 
BmgBI CAC'GTC

Cuts at:      0     270     480    1383 
   Size:        270     210     903

  Fragments arranged by size: 

                 903     270     210
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     593    1383 
   Size:        593     790
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     954    1171    1383 
   Size:         88     866     217     212

  Fragments arranged by size: 

                 866     217     212      88
 
Bpu10I CC'TnA_GC

Cuts at:      0     239    1108    1383 
   Size:        239     869     275

  Fragments arranged by size: 

                 869     275     239
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     378    1383 
   Size:        378    1005
 
BsaHI Gr'CG_yC

Cuts at:      0     797    1383 
   Size:        797     586
 
BsaWI w'CCGG_w

Cuts at:      0     509    1383 
   Size:        509     874
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     366     378     829     832     919     922    1189 
   Size:        274      92      12     451       3      87       3     267

Cuts at:   1189    1321    1383 
   Size:        132      62

  Fragments arranged by size: 

                 451     274     267     132      92      87      62      12
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0    1335    1383 
   Size:       1335      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     586    1383 
   Size:        478     108     797

  Fragments arranged by size: 

                 797     478     108
 
BsiEI CG_ry'CG

Cuts at:      0      37     134     143    1383 
   Size:         37      97       9    1240

  Fragments arranged by size: 

                1240      97      37       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     782     914    1211    1383 
   Size:        782     132     297     172

  Fragments arranged by size: 

                 782     297     172     132
 
Bsp1286I G_dGCh'C

Cuts at:      0      19      78     161     168     219     266     275     331 
   Size:         19      59      83       7      51      47       9      56

Cuts at:    331     581     607     782     914    1211    1383 
   Size:        250      26     175     132     297     172

  Fragments arranged by size: 

                 297     250     175     172     132      83      59      56
                  51      47      26      19       9       7
 
BspEI T'CCGG_A

Cuts at:      0     509    1383 
   Size:        509     874
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     347     428     743     758    1050    1383 
   Size:        347      81     315      15     292     333

  Fragments arranged by size: 

                 347     333     315     292      81      15
 
BsrBI CCG'CTC

Cuts at:      0      72     300     840    1383 
   Size:         72     228     540     543

  Fragments arranged by size: 

                 543     540     228      72
 
BsrFI r'CCGG_y

Cuts at:      0     528    1228    1292    1383 
   Size:        528     700      64      91

  Fragments arranged by size: 

                 700     528      91      64
 
BsrGI T'GTAC_A

Cuts at:      0     863    1383 
   Size:        863     520
 
BssHII G'CGCG_C

Cuts at:      0     118     921    1383 
   Size:        118     803     462

  Fragments arranged by size: 

                 803     462     118
 
BssSI C'ACGA_G

Cuts at:      0    1177    1383 
   Size:       1177     206
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     750    1383 
   Size:        750     633
 
BstYI r'GATC_y

Cuts at:      0     506     885    1136    1280    1383 
   Size:        506     379     251     144     103

  Fragments arranged by size: 

                 506     379     251     144     103
 
BtgI C'CryG_G

Cuts at:      0     149     168     211     447     515     702     899    1383 
   Size:        149      19      43     236      68     187     197     484

  Fragments arranged by size: 

                 484     236     197     187     149      68      43      19
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1207    1383 
   Size:       1207     176
 
BtsI GCAGTG_nn'

Cuts at:      0     955    1215    1383 
   Size:        955     260     168

  Fragments arranged by size: 

                 955     260     168
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     165     200    1383 
   Size:        165      35    1183

  Fragments arranged by size: 

                1183     165      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     708    1182    1383 
   Size:        708     474     201

  Fragments arranged by size: 

                 708     474     201
 
EaeI y'GGCC_r

Cuts at:      0      34     140    1275    1364    1383 
   Size:         34     106    1135      89      19

  Fragments arranged by size: 

                1135     106      89      34      19
 
EagI C'GGCC_G

Cuts at:      0      34     140    1383 
   Size:         34     106    1243

  Fragments arranged by size: 

                1243     106      34
 
EarI CTCTTCn'nnn_

Cuts at:      0     115     336    1073    1383 
   Size:        115     221     737     310

  Fragments arranged by size: 

                 737     310     221     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     643     911     954    1152    1171    1383 
   Size:         88     555     268      43     198      19     212

  Fragments arranged by size: 

                 555     268     212     198      88      43      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     814    1383 
   Size:        814     569
 
EcoO109I rG'GnC_Cy

Cuts at:      0      15      50      75     195     747    1383 
   Size:         15      35      25     120     552     636

  Fragments arranged by size: 

                 636     552     120      35      25      15
 
HaeII r_GCGC'y

Cuts at:      0     555     630     648    1383 
   Size:        555      75      18     735

  Fragments arranged by size: 

                 735     555      75      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1101    1133    1383 
   Size:       1101      32     250

  Fragments arranged by size: 

                1101     250      32
 
MscI TGG'CCA

Cuts at:      0    1277    1366    1383 
   Size:       1277      89      17

  Fragments arranged by size: 

                1277      89      17
 
MspA1I CmG'CkG

Cuts at:      0     151     170     213     243     325     449     462     570 
   Size:        151      19      43      30      82     124      13     108

Cuts at:    570     611     741    1383 
   Size:         41     130     642

  Fragments arranged by size: 

                 642     151     130     124     108      82      43      41
                  30      19      13
 
NaeI GCC'GGC

Cuts at:      0    1230    1383 
   Size:       1230     153
 
NcoI C'CATG_G

Cuts at:      0     515    1383 
   Size:        515     868
 
NgoMIV G'CCGG_C

Cuts at:      0    1228    1383 
   Size:       1228     155
 
NotI GC'GGCC_GC

Cuts at:      0     140    1383 
   Size:        140    1243
 
NspI r_CATG'y

Cuts at:      0     864     948    1383 
   Size:        864      84     435

  Fragments arranged by size: 

                 864     435      84
 
PasI CC'CwG_GG

Cuts at:      0     286    1301    1383 
   Size:        286    1015      82

  Fragments arranged by size: 

                1015     286      82
 
PciI A'CATG_T

Cuts at:      0     860    1383 
   Size:        860     523
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     708    1284    1383 
   Size:        708     576      99

  Fragments arranged by size: 

                 708     576      99
 
PfoI T'CCnGG_A

Cuts at:      0    1139    1283    1383 
   Size:       1139     144     100

  Fragments arranged by size: 

                1139     144     100
 
PpuMI rG'GwC_Cy

Cuts at:      0     195     747    1383 
   Size:        195     552     636

  Fragments arranged by size: 

                 636     552     195
 
PshAI GACnn'nnGTC

Cuts at:      0     482     517    1383 
   Size:        482      35     866

  Fragments arranged by size: 

                 866     482      35
 
PspOMI G'GGCC_C

Cuts at:      0      15      74     164    1383 
   Size:         15      59      90    1219

  Fragments arranged by size: 

                1219      90      59      15
 
PspXI vC'TCGA_Gb

Cuts at:      0     400    1383 
   Size:        400     983
 
PstI C_TGCA'G

Cuts at:      0      58     344     740     746    1027    1133    1383 
   Size:         58     286     396       6     281     106     250

  Fragments arranged by size: 

                 396     286     281     250     106      58       6
 
PvuII CAG'CTG

Cuts at:      0     243     462     570     741    1383 
   Size:        243     219     108     171     642

  Fragments arranged by size: 

                 642     243     219     171     108
 
SacII CC_GC'GG

Cuts at:      0     152     171     214     450    1383 
   Size:        152      19      43     236     933

  Fragments arranged by size: 

                 933     236     152      43      19
 
SapI GCTCTTCn'nnn_

Cuts at:      0     115     336    1383 
   Size:        115     221    1047

  Fragments arranged by size: 

                1047     221     115
 
SfcI C'TryA_G

Cuts at:      0      54     340     736     742    1023    1129    1383 
   Size:         54     286     396       6     281     106     254

  Fragments arranged by size: 

                 396     286     281     254     106      54       6
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     172    1383 
   Size:        172    1211
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1228    1292    1383 
   Size:       1228      64      91

  Fragments arranged by size: 

                1228      91      64
 
SmaI CCC'GGG

Cuts at:      0      12      62     163    1383 
   Size:         12      50     101    1220

  Fragments arranged by size: 

                1220     101      50      12
 
SmlI C'TyrA_G

Cuts at:      0     400    1383 
   Size:        400     983
 
StyI C'CwwG_G

Cuts at:      0     515     827    1383 
   Size:        515     312     556

  Fragments arranged by size: 

                 556     515     312
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     360     552    1383 
   Size:        360     192     831

  Fragments arranged by size: 

                 831     360     192
 
TatI w'GTAC_w

Cuts at:      0     863    1383 
   Size:        863     520
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     761     793    1383 
   Size:        761      32     590

  Fragments arranged by size: 

                 761     590      32
 
XhoI C'TCGA_G

Cuts at:      0     400    1383 
   Size:        400     983
 
XmaI C'CCGG_G

Cuts at:      0      10      60     161    1383 
   Size:         10      50     101    1222

  Fragments arranged by size: 

                1222     101      50      10
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    AlwNI     ApaI    ApaLI     AvaI
    BamHI     BanI    BanII    BbvCI     BcgI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstYI     BtgI
    BtgZI     BtsI    CspCI   DraIII     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I    HaeII    Hin4I     MscI   MspA1I     NaeI     NcoI
   NgoMIV     NotI     NspI     PasI     PciI    PflMI     PfoI    PpuMI
    PshAI   PspOMI    PspXI     PstI    PvuII    SacII     SapI     SfcI
     SfiI    SgrAI     SmaI     SmlI     StyI    TaqII     TatI     TstI
     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI     BbsI     BclI    BfrBI    BglII     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaXI    BsiWI     BsmI    BsmBI    BspHI    BsrDI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI     DraI     DrdI
     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NarI     NdeI     NheI     NruI     NsiI     PacI     PmeI
     PmlI     PpiI     PsiI     PsrI     PvuI    RsrII     SacI     SalI
    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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