Restriction map for
pF1KB7758
(Circular) (Six-base) MAPSORT of: pF1KB7758.seq Check: 5255 from: 1 to: 4607
pF1KB7758 4607 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1099 1099
Size: 4607
AatII G_ACGT'C
Cuts at: 872 872
Size: 4607
AccI GT'mk_AC
Cuts at: 128 1645 128
Size: 1517 3090
Acc65I G'GTAC_C
Cuts at: 1623 1623
Size: 4607
AclI AA'CG_TT
Cuts at: 4447 4447
Size: 4607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2432 2864 3611 3879 2432
Size: 432 747 268 3160
Fragments arranged by size:
3160 747 432 268
AflIII A'CryG_T
Cuts at: 1351 1959 3063 4040 1351
Size: 608 1104 977 1918
Fragments arranged by size:
1918 1104 977 608
AgeI A'CCGG_T
Cuts at: 2995 2995
Size: 4607
AlwNI CAG_nnn'CTG
Cuts at: 209 558 1912 3479 209
Size: 349 1354 1567 1337
Fragments arranged by size:
1567 1354 1337 349
ApaLI G'TGCA_C
Cuts at: 3377 3377
Size: 4607
ApoI r'AATT_y
Cuts at: 1104 1611 3960 4216 1104
Size: 507 2349 256 1495
Fragments arranged by size:
2349 1495 507 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4607
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
AvaI C'yCGr_G
Cuts at: 1627 1627
Size: 4607
BamHI G'GATC_C
Cuts at: 1208 1632 1208
Size: 424 4183
BanI G'GyrC_C
Cuts at: 1623 2037 2285 2320 1623
Size: 414 248 35 3910
Fragments arranged by size:
3910 414 248 35
BanII G_rGCy'C
Cuts at: 170 370 1258 1600 1621 2651 170
Size: 200 888 342 21 1030 2126
Fragments arranged by size:
2126 1030 888 342 200 21
BbeI G_GCGC'C
Cuts at: 2041 2289 2041
Size: 248 4359
BbsI GAAGACnn'nnnn_
Cuts at: 1131 1131
Size: 4607
BciVI GTATCCnnnnn_n'
Cuts at: 2505 3266 4607 2505
Size: 761 1341 2505
Fragments arranged by size:
2505 1341 761
BclI T'GATC_A
Cuts at: 248 2127 248
Size: 1879 2728
BglII A'GATC_T
Cuts at: 422 813 951 2122 4159 422
Size: 391 138 1171 2037 870
Fragments arranged by size:
2037 1171 870 391 138
BlpI GC'TnA_GC
Cuts at: 1719 1719
Size: 4607
Bme1580I G_kGCm'C
Cuts at: 1591 2232 2325 3381 1591
Size: 641 93 1056 2817
Fragments arranged by size:
2817 1056 641 93
BmgBI CAC'GTC
Cuts at: 1014 1014
Size: 4607
BmrI ACTGGGnnnn_n'
Cuts at: 1014 1185 1338 1819 1900 2235 4379 1014
Size: 171 153 481 81 335 2144 1242
Fragments arranged by size:
2144 1242 481 335 171 153 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1395 2974 1395
Size: 1579 3028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 403 742 802 1287 184
Size: 219 339 60 485 3504
Fragments arranged by size:
3504 485 339 219 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 240 1783 2662 3154 3452 240
Size: 1543 879 492 298 1395
Fragments arranged by size:
1543 1395 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4311 4311
Size: 4607
BsaAI yAC'GTr
Cuts at: 1960 1960
Size: 4607
BsaBI GATnn'nnATC
Cuts at: 6 2121 4158 6
Size: 2115 2037 455
Fragments arranged by size:
2115 2037 455
BsaHI Gr'CG_yC
Cuts at: 869 2038 2286 2988 4484 869
Size: 1169 248 702 1496 992
Fragments arranged by size:
1496 1169 992 702 248
BsaWI w'CCGG_w
Cuts at: 1796 2317 2995 3269 3416 3827 1796
Size: 521 678 274 147 411 2576
Fragments arranged by size:
2576 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 712 1368 1371 712
Size: 656 3 3948
Fragments arranged by size:
3948 656 3
BseYI C'CCAG_C
Cuts at: 455 2032 2790 3367 3863 455
Size: 1577 758 577 496 1199
Fragments arranged by size:
1577 1199 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1077 1077
Size: 4607
BsiEI CG_ry'CG
Cuts at: 104 2149 2195 3403 104
Size: 2045 46 1208 1308
Fragments arranged by size:
2045 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 170 404 1258 1500 1600 1621 2399 2589 3381
Size: 234 854 242 100 21 778 190 792
Cuts at: 3381 170
Size: 1396
Fragments arranged by size:
1396 854 792 778 242 234 190 100
21
Bsp1286I G_dGCh'C
Cuts at: 170 370 404 1258 1500 1591 1600 1621 2232
Size: 200 34 854 242 91 9 21 611
Cuts at: 2232 2325 2399 2589 2651 3381 170
Size: 93 74 190 62 730 1396
Fragments arranged by size:
1396 854 730 611 242 200 190 93
91 74 62 34 21 9
BspEI T'CCGG_A
Cuts at: 3827 3827
Size: 4607
BspHI T'CATG_A
Cuts at: 872 3783 872
Size: 2911 1696
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 531 1099 1545 1657 1871 2173 2554 157
Size: 374 568 446 112 214 302 381 2210
Fragments arranged by size:
2210 568 446 381 374 302 214 112
BsrBI CCG'CTC
Cuts at: 50 2899 2953 4437 4602 50
Size: 2849 54 1484 165 55
Fragments arranged by size:
2849 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2519 2519
Size: 4607
BsrFI r'CCGG_y
Cuts at: 2605 2786 2995 2605
Size: 181 209 4217
Fragments arranged by size:
4217 209 181
BssSI C'ACGA_G
Cuts at: 2878 3236 2878
Size: 358 4249
BstAPI GCAn_nnn'nTGC
Cuts at: 1984 1984
Size: 4607
BstBI TT'CG_AA
Cuts at: 1042 2968 1042
Size: 1926 2681
BstEII G'GTnAC_C
Cuts at: 144 487 144
Size: 343 4264
BstXI CCAn_nnnn'nTGG
Cuts at: 162 162
Size: 4607
BstYI r'GATC_y
Cuts at: 1 422 813 951 1208 1632 2099 2122 2457
Size: 421 391 138 257 424 467 23 335
Cuts at: 2457 2703 3715 3801 3813 4159 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 424 421 391 346 335
257 246 138 86 23 12
BtgI C'CryG_G
Cuts at: 107 405 1491 107
Size: 298 1086 3223
Fragments arranged by size:
3223 1086 298
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2557 2738 2901 2557
Size: 181 163 4263
Fragments arranged by size:
4263 181 163
BtsI GCAGTG_nn'
Cuts at: 224 1038 1850 224
Size: 814 812 2981
Fragments arranged by size:
2981 814 812
DraI TTT'AAA
Cuts at: 1606 1606
Size: 4607
DrdI GACnn_nn'nnGTC
Cuts at: 775 2313 3171 775
Size: 1538 858 2211
Fragments arranged by size:
2211 1538 858
EaeI y'GGCC_r
Cuts at: 198 329 2192 2366 2757 2784 4129 198
Size: 131 1863 174 391 27 1345 676
Fragments arranged by size:
1863 1345 676 391 174 131 27
EagI C'GGCC_G
Cuts at: 2192 2192
Size: 4607
EarI CTCTTCn'nnn_
Cuts at: 379 1164 1246 1389 2630 2840 379
Size: 785 82 143 1241 210 2146
Fragments arranged by size:
2146 1241 785 210 143 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2815 3125 3271 4417 2815
Size: 310 146 1146 3005
Fragments arranged by size:
3005 1146 310 146
EcoICRI GAG'CTC
Cuts at: 168 1256 1598 1619 168
Size: 1088 342 21 3156
Fragments arranged by size:
3156 1088 342 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 403 742 802 1287 2432 2864 3611 3879
Size: 219 339 60 485 1145 432 747 268
Cuts at: 3879 184
Size: 912
Fragments arranged by size:
1145 912 747 485 432 339 268 219
60
EcoO109I rG'GnC_Cy
Cuts at: 1746 1746
Size: 4607
EcoRI G'AATT_C
Cuts at: 1104 1611 1104
Size: 507 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 4607
FspI TGC'GCA
Cuts at: 2388 2388
Size: 4607
HaeII r_GCGC'y
Cuts at: 2041 2289 3311 4290 2041
Size: 248 1022 979 2358
Fragments arranged by size:
2358 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 165 197 1008 1040 1366 1398 1487 1519 2449
Size: 32 811 32 326 32 89 32 930
Cuts at: 2449 2479 2481 2511 165
Size: 30 2 30 2261
Fragments arranged by size:
2261 930 811 326 89 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1646 1646
Size: 4607
KasI G'GCGC_C
Cuts at: 2037 2285 2037
Size: 248 4359
KpnI G_GTAC'C
Cuts at: 1627 1627
Size: 4607
MluI A'CGCG_T
Cuts at: 4040 4040
Size: 4607
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 336 3278 3462 336
Size: 2942 184 1481
Fragments arranged by size:
2942 1481 184
MscI TGG'CCA
Cuts at: 200 331 2368 200
Size: 131 2037 2439
Fragments arranged by size:
2439 2037 131
MslI CAynn'nnrTG
Cuts at: 1582 2596 2723 1582
Size: 1014 127 3466
Fragments arranged by size:
3466 1014 127
MspA1I CmG'CkG
Cuts at: 272 774 822 1718 2032 2392 3405 3650 272
Size: 502 48 896 314 360 1013 245 1229
Fragments arranged by size:
1229 1013 896 502 360 314 245 48
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 4607
NarI GG'CG_CC
Cuts at: 2038 2286 2038
Size: 248 4359
NcoI C'CATG_G
Cuts at: 107 405 107
Size: 298 4309
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 4607
NspI r_CATG'y
Cuts at: 1355 1660 2160 3067 1355
Size: 305 500 907 2895
Fragments arranged by size:
2895 907 500 305
PciI A'CATG_T
Cuts at: 1351 3063 1351
Size: 1712 2895
PmeI GTTT'AAAC
Cuts at: 1606 1606
Size: 4607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4575
PshAI GACnn'nnGTC
Cuts at: 991 991
Size: 4607
PstI C_TGCA'G
Cuts at: 542 554 857 1654 2339 542
Size: 12 303 797 685 2810
Fragments arranged by size:
2810 797 685 303 12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4607
PvuII CAG'CTG
Cuts at: 272 774 2032 2392 272
Size: 502 1258 360 2487
Fragments arranged by size:
2487 1258 502 360
RsrII CG'GwC_CG
Cuts at: 2802 2802
Size: 4607
SacI G_AGCT'C
Cuts at: 170 1258 1600 1621 170
Size: 1088 342 21 3156
Fragments arranged by size:
3156 1088 342 21
SalI G'TCGA_C
Cuts at: 1644 1644
Size: 4607
SapI GCTCTTCn'nnn_
Cuts at: 1246 2630 2840 1246
Size: 1384 210 3013
Fragments arranged by size:
3013 1384 210
SbfI CC_TGCA'GG
Cuts at: 1654 1654
Size: 4607
ScaI AGT'ACT
Cuts at: 1000 4124 1000
Size: 3124 1483
SfcI C'TryA_G
Cuts at: 33 538 550 764 853 1650 2335 3328 3519
Size: 505 12 214 89 797 685 993 191
Cuts at: 3519 33
Size: 1121
Fragments arranged by size:
1121 993 797 685 505 214 191 89
12
SfoI GGC'GCC
Cuts at: 2039 2287 2039
Size: 248 4359
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4607
SmaI CCC'GGG
Cuts at: 1629 1629
Size: 4607
SmlI C'TyrA_G
Cuts at: 255 1762 2677 3169 3431 255
Size: 1507 915 492 262 1431
Fragments arranged by size:
1507 1431 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4607
SphI G_CATG'C
Cuts at: 1660 2160 1660
Size: 500 4107
SspI AAT'ATT
Cuts at: 1153 1171 1153
Size: 18 4589
StyI C'CwwG_G
Cuts at: 107 405 483 1394 1558 1741 2095 107
Size: 298 78 911 164 183 354 2619
Fragments arranged by size:
2619 911 354 298 183 164 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 996 2190 2323 2782 2991 996
Size: 1194 133 459 209 2612
Fragments arranged by size:
2612 1194 459 209 133
TatI w'GTAC_w
Cuts at: 998 4122 998
Size: 3124 1483
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4124 4156 4124
Size: 32 4575
Tth111I GACn'n_nGTC
Cuts at: 2404 2404
Size: 4607
XbaI T'CTAG_A
Cuts at: 1638 1638
Size: 4607
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2102 2102
Size: 4607
XmaI C'CCGG_G
Cuts at: 1627 1627
Size: 4607
XmnI GAAnn'nnTTC
Cuts at: 664 664
Size: 4607
ZraI GAC'GTC
Cuts at: 870 870
Size: 4607
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI