Restriction map for pF1KB7745
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       ApoI        AscI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BciVI       BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    Eco47III    Eco81I      EcoO65I     EcoRI       
EcoT22I     FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        TfiI        Tsp509I     Tth111I     
Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          898  GTCTAC               GT^[AC][GT]AC
AccII          60  CGCG                 CG^CG
AfaI          144  GTAC                 GT^AC
AlwI          267  GGATC                GGATC(4/5)
AseI         1306  ATTAAT               AT^TAAT
AsnI         1306  ATTAAT               AT^TAAT
AspHI         876  GAGCAC               G[AT]GC[AT]^C
BalI           82  TGGCCA               TGG^CCA
BclI         1199  TGATCA               T^GATCA
BfaI         1016  CTAG                 C^TAG
BglI          802  GCCGTCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI          29  CGGCCG               CG[GA][CT]^CG
BsiHKAI       876  GAGCAC               G[AT]GC[AT]^C
BsmAI         825  GAGAC                GTCTC(1/5)
BstUI          60  CGCG                 CG^CG
BstXI         518  CCAACGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         267  GGATCT               [GA]^GATC[CT]
DsaI          201  CCACGG               C^C[GA][CT]GG
EagI           29  CGGCCG               C^GGCCG
EarI         1273  CTCTTC               CTCTTC(1/4)
EclXI          29  CGGCCG               C^GGCCG
Eco52I         29  CGGCCG               C^GGCCG
EcoNI         711  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         760  GATATC               GAT^ATC
FbaI         1199  TGATCA               T^GATCA
HgaI          190  GACGC                GACGC(5/10)
Ksp632I      1273  CTCTTC               CTCTTC(1/4)
MaeI         1016  CTAG                 C^TAG
MaeIII        748  GTGAC                ^GT[ACGT]AC
MflI          267  GGATCT               [GA]^GATC[CT]
MluNI          82  TGGCCA               TGG^CCA
MscI           82  TGGCCA               TGG^CCA
MvnI           60  CGCG                 CG^CG
NlaIII       1023  CATG                 CATG^
PshBI        1306  ATTAAT               AT^TAAT
PvuII         625  CAGCTG               CAG^CTG
RsaI          144  GTAC                 GT^AC
SmlI          871  CTCAAG               C^T[CT][GA]AG
StuI          665  AGGCCT               AGG^CCT
Tsp45I        748  GTGAC                ^GT[GC]AC
XhoII         267  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI           10  GGGCCC               GGGCC^C
              886  GGGCCC              
BbeI          156  GGCGCC               GGCGC^C
              171  GGCGCC              
BbsI          378  GAAGAC               GAAGAC(2/6)
              595  GAAGAC              
BbvCI          93  CCTCAGC              CCTCAGC(-5/-2)
              475  GCTGAGG             
BcgI           47  CGAAGGTGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              179  GCACCTGCCTCG        
BpmI          271  CTGGAG               CTGGAG(16/14)
             1123  CTGGAG              
BpuAI         378  GAAGAC               GAAGAC(2/6)
              595  GAAGAC              
BseRI         913  GAGGAG               GAGGAG(10/8)
              943  GAGGAG              
BspMI         181  ACCTGC               ACCTGC(4/8)
              857  ACCTGC              
KasI          156  GGCGCC               G^GCGCC
              171  GGCGCC              
MaeII         488  ACGT                 A^CGT
              521  ACGT                
MseI          782  TTAA                 T^TAA
             1307  TTAA                
MslI          327  CATCAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              516  CACCAACGTG          
NarI          156  GGCGCC               GG^CGCC
              171  GGCGCC              
PpuMI         452  GGGTCCT              [GA]G^G[AT]CC[CT]
             1295  GGGACCT             
PstI          859  CTGCAG               CTGCA^G
             1144  CTGCAG              
SfcI          859  CTGCAG               C^T[GA][CT]AG
             1144  CTGCAG              
SfoI          156  GGCGCC               GGC^GCC
              171  GGCGCC              
SmaI           14  CCCGGG               CCC^GGG
              370  CCCGGG              
Tru9I         782  TTAA                 T^TAA
             1307  TTAA                
XcmI          254  CCAAAAGGAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              728  CCAACAAGAGGCTGG     
XmaCI          14  CCCGGG               C^CCGGG
              370  CCCGGG              
XmaI           14  CCCGGG               C^CCGGG
              370  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AcyI        AluI        AlwNI       AvaI        
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsgI        BsiYI       
BslI        BsmFI       BsoBI       Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
StyI        TaqI        TseI        TspRI       TthHB8I     
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