Restriction map for pF1KB7745
 (Linear) (Six-base) MAPSORT of: KIBB7745.seq  Check: 6053  from: 1  to: 1311



KIBB7745.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     190    1311 
   Size:        190    1121
 
AccI GT'mk_AC

Cuts at:      0     899    1311 
   Size:        899     412
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661    1311 
   Size:        661     650
 
AleI CACnn'nnGTG

Cuts at:      0     520    1311 
   Size:        520     791
 
AlwNI CAG_nnn'CTG

Cuts at:      0     121     639    1167    1311 
   Size:        121     518     528     144

  Fragments arranged by size: 

                 528     518     144     121
 
ApaI G_GGCC'C

Cuts at:      0      14     890    1311 
   Size:         14     876     421

  Fragments arranged by size: 

                 876     421      14
 
AseI AT'TA_AT

Cuts at:      0    1307    1311 
   Size:       1307       4
 
AvaI C'yCGr_G

Cuts at:      0      14     208     370    1311 
   Size:         14     194     162     941

  Fragments arranged by size: 

                 941     194     162      14
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     127     160    1311 
   Size:        127      33    1151

  Fragments arranged by size: 

                1151     127      33
 
BanI G'GyrC_C

Cuts at:      0      54     156     171     178    1311 
   Size:         54     102      15       7    1133

  Fragments arranged by size: 

                1133     102      54      15       7
 
BanII G_rGCy'C

Cuts at:      0      14      70     890    1031    1311 
   Size:         14      56     820     141     280

  Fragments arranged by size: 

                 820     280     141      56      14
 
BbeI G_GCGC'C

Cuts at:      0     160     175    1311 
   Size:        160      15    1136

  Fragments arranged by size: 

                1136     160      15
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     385     602    1311 
   Size:        385     217     709

  Fragments arranged by size: 

                 709     385     217
 
BbvCI CC'TCA_GC

Cuts at:      0      94     476    1311 
   Size:         94     382     835

  Fragments arranged by size: 

                 835     382      94
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      36      70     168     202    1311 
   Size:         36      34      98      34    1109

  Fragments arranged by size: 

                1109      98      36      34      34
 
BclI T'GATC_A

Cuts at:      0    1199    1311 
   Size:       1199     112
 
BglI GCCn_nnn'nGGC

Cuts at:      0     808    1311 
   Size:        808     503
 
Bme1580I G_kGCm'C

Cuts at:      0      14      59     839     890     910    1311 
   Size:         14      45     780      51      20     401

  Fragments arranged by size: 

                 780     401      51      45      20      14
 
BmgBI CAC'GTC

Cuts at:      0     489    1311 
   Size:        489     822
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     477    1311 
   Size:        477     834
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     710    1311 
   Size:        710     601
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292    1144    1311 
   Size:        292     852     167

  Fragments arranged by size: 

                 852     292     167
 
Bpu10I CC'TnA_GC

Cuts at:      0      94     433     476    1311 
   Size:         94     339      43     835

  Fragments arranged by size: 

                 835     339      94      43
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856    1311 
   Size:        856     455
 
BsaHI Gr'CG_yC

Cuts at:      0     157     172     191    1311 
   Size:        157      15      19    1120

  Fragments arranged by size: 

                1120     157      19      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     144     174    1311 
   Size:        144      30    1137

  Fragments arranged by size: 

                1137     144      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     928     958    1311 
   Size:        928      30     353

  Fragments arranged by size: 

                 928     353      30
 
BseYI C'CCAG_C

Cuts at:      0      34     104     114     346     399     627     976     992 
   Size:         34      70      10     232      53     228     349      16

Cuts at:    992     997    1067    1311 
   Size:          5      70     244

  Fragments arranged by size: 

                 349     244     232     228      70      70      53      34
                  16      10       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     940    1206    1311 
   Size:        511     429     266     105

  Fragments arranged by size: 

                 511     429     266     105
 
BsiEI CG_ry'CG

Cuts at:      0      32    1311 
   Size:         32    1279
 
BsiHKAI G_wGCw'C

Cuts at:      0     880    1311 
   Size:        880     431
 
Bsp1286I G_dGCh'C

Cuts at:      0      14      59      70     839     880     890     910    1031 
   Size:         14      45      11     769      41      10      20     121

Cuts at:   1031    1311 
   Size:        280

  Fragments arranged by size: 

                 769     280     121      45      41      20      14      11
                  10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     190     866    1311 
   Size:        190     676     445

  Fragments arranged by size: 

                 676     445     190
 
BsrFI r'CCGG_y

Cuts at:      0     234     242     249    1311 
   Size:        234       8       7    1062

  Fragments arranged by size: 

                1062     234       8       7
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     525    1311 
   Size:        525     786
 
BstYI r'GATC_y

Cuts at:      0     267    1311 
   Size:        267    1044
 
BtgI C'CryG_G

Cuts at:      0     201    1311 
   Size:        201    1110
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      76     240    1311 
   Size:         76     164    1071

  Fragments arranged by size: 

                1071     164      76
 
EaeI y'GGCC_r

Cuts at:      0      29      82     251    1311 
   Size:         29      53     169    1060

  Fragments arranged by size: 

                1060     169      53      29
 
EagI C'GGCC_G

Cuts at:      0      29    1311 
   Size:         29    1282
 
EarI CTCTTCn'nnn_

Cuts at:      0    1279    1311 
   Size:       1279      32
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     661    1144    1311 
   Size:        292     369     483     167

  Fragments arranged by size: 

                 483     369     292     167
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     715    1311 
   Size:        715     596
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10      11     453     810     886    1074    1296    1311 
   Size:         10       1     442     357      76     188     222      15

  Fragments arranged by size: 

                 442     357     222     188      76      15      10       1
            
 
EcoRV GAT'ATC

Cuts at:      0     762    1311 
   Size:        762     549
 
HaeII r_GCGC'y

Cuts at:      0     160     175    1060    1311 
   Size:        160      15     885     251

  Fragments arranged by size: 

                 885     251     160      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     176    1311 
   Size:        144      32    1135

  Fragments arranged by size: 

                1135     144      32
 
KasI G'GCGC_C

Cuts at:      0     156     171    1311 
   Size:        156      15    1140

  Fragments arranged by size: 

                1140     156      15
 
MscI TGG'CCA

Cuts at:      0      84    1311 
   Size:         84    1227
 
MslI CAynn'nnrTG

Cuts at:      0     331     520    1311 
   Size:        331     189     791

  Fragments arranged by size: 

                 791     331     189
 
MspA1I CmG'CkG

Cuts at:      0      34     141     169     475     627    1311 
   Size:         34     107      28     306     152     684

  Fragments arranged by size: 

                 684     306     152     107      34      28
 
NaeI GCC'GGC

Cuts at:      0     236     244     251    1311 
   Size:        236       8       7    1060

  Fragments arranged by size: 

                1060     236       8       7
 
NarI GG'CG_CC

Cuts at:      0     157     172    1311 
   Size:        157      15    1139

  Fragments arranged by size: 

                1139     157      15
 
NgoMIV G'CCGG_C

Cuts at:      0     234     242     249    1311 
   Size:        234       8       7    1062

  Fragments arranged by size: 

                1062     234       8       7
 
PfoI T'CCnGG_A

Cuts at:      0     455    1311 
   Size:        455     856
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1147    1179    1311 
   Size:       1147      32     132

  Fragments arranged by size: 

                1147     132      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     453    1296    1311 
   Size:        453     843      15

  Fragments arranged by size: 

                 843     453      15
 
PspOMI G'GGCC_C

Cuts at:      0      10     886    1311 
   Size:         10     876     425

  Fragments arranged by size: 

                 876     425      10
 
PstI C_TGCA'G

Cuts at:      0     863    1148    1311 
   Size:        863     285     163

  Fragments arranged by size: 

                 863     285     163
 
PvuII CAG'CTG

Cuts at:      0     627    1311 
   Size:        627     684
 
SfcI C'TryA_G

Cuts at:      0     859    1144    1311 
   Size:        859     285     167

  Fragments arranged by size: 

                 859     285     167
 
SfoI GGC'GCC

Cuts at:      0     158     173    1311 
   Size:        158      15    1138

  Fragments arranged by size: 

                1138     158      15
 
SmaI CCC'GGG

Cuts at:      0      16     372    1311 
   Size:         16     356     939

  Fragments arranged by size: 

                 939     356      16
 
SmlI C'TyrA_G

Cuts at:      0     871    1311 
   Size:        871     440
 
StuI AGG'CCT

Cuts at:      0     667    1311 
   Size:        667     644
 
StyI C'CwwG_G

Cuts at:      0      20     314     668     882    1100    1217    1290    1311 
   Size:         20     294     354     214     218     117      73      21

  Fragments arranged by size: 

                 354     294     218     214     117      73      21      20
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     895    1151    1311 
   Size:        895     256     160

  Fragments arranged by size: 

                 895     256     160
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     261     735    1311 
   Size:        261     474     576

  Fragments arranged by size: 

                 576     474     261
 
XmaI C'CCGG_G

Cuts at:      0      14     370    1311 
   Size:         14     356     941

  Fragments arranged by size: 

                 941     356      14
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI    AlwNI     ApaI     AseI     AvaI
     BaeI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspMI    BsrFI    BstXI    BstYI     BtgI    BtgZI     EaeI     EagI
     EarI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I     KasI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     PfoI     PpiI
    PpuMI   PspOMI     PstI    PvuII     SfcI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI    AsiSI    AvrII    BamHI
    BciVI    BfrBI    BglII     BlpI     BmtI     BsaI    BsaAI    BsaBI
    BsaWI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoRI     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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