Restriction map for pF1KB7744
##################################
# NO CUT ENZYMES (  109 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
BanI        BbeI        BbsI        BcgI        BciVI       
BfrI        BglII       BpuAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MslI        MunI        NarI        NcoI        NdeI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       Tsp509I     


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII        1342  CGCG                 CG^CG
Aor51HI      1223  AGCGCT               AGC^GCT
ApaI          969  GGGCCC               GGGCC^C
Asp700I      1194  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          564  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         677  TGCGCA               TGC^GCA
AvrII         733  CCTAGG               C^CTAGG
BalI          600  TGGCCA               TGG^CCA
BamHI         881  GGATCC               G^GATCC
BbrPI         636  CACGTG               CAC^GTG
BlnI          733  CCTAGG               C^CTAGG
BsaAI         636  CACGTG               [CT]AC^GT[GA]
BsaI         1106  GGTCTC               GGTCTC(1/5)
BsgI          304  GTGCAG               GTGCAG(16/14)
BsmI          818  GCATTC               GAATGC(1/-1)
BspHI        1047  TCATGA               T^CATGA
BsrFI        1303  GCCGGC               [GA]^CCGG[CT]
BssSI         741  CTCGTG               CACGAG(-5/-1)
BstUI        1342  CGCG                 CG^CG
BstXI         290  CCAGGGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I       1303  GCCGGC               [GA]^CCGG[CT]
DraIII         80  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1223  AGCGCT               AGC^GCT
FspI          677  TGCGCA               TGC^GCA
HaeII        1223  AGCGCT               [GA]GCGC^[CT]
MaeII         637  ACGT                 A^CGT
MluNI         600  TGGCCA               TGG^CCA
MscI          600  TGGCCA               TGG^CCA
MvnI         1342  CGCG                 CG^CG
NaeI         1303  GCCGGC               GCC^GGC
NgoMIV       1303  GCCGGC               G^CCGGC
PflFI         564  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         636  CACGTG               CAC^GTG
PmlI          636  CACGTG               CAC^GTG
PpuMI         428  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          253  CTGCAG               CTGCA^G
PvuII         157  CAGCTG               CAG^CTG
RcaI         1047  TCATGA               T^CATGA
SexAI         565  ACCAGGT              A^CC[AT]GGT
SmaI         1229  CCCGGG               CCC^GGG
SphI          322  GCATGC               GCATG^C
StuI          366  AGGCCT               AGG^CCT
TfiI          408  GAATC                G^A[AT]TC
Tth111I       564  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          463  TCTAGA               T^CTAGA
XcmI          588  CCAAGCCCCTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1229  CCCGGG               C^CCGGG
XmaI         1229  CCCGGG               C^CCGGG
XmnI         1194  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AfaI           52  GTAC                 GT^AC
             1281  GTAC                
AspHI         631  GTGCTC               G[AT]GC[AT]^C
             1122  GTGCTC              
AvaII         429  GGACC                G^G[AT]CC
             1376  GGACC               
BbvCI        1219  CCTCAGC              CCTCAGC(-5/-2)
              748  GCTGAGG             
BclI           95  TGATCA               T^GATCA
             1008  TGATCA              
BlpI          550  GCTCAGC              GC^T[ACGT]AGC
              981  GCTCAGC             
Bpu1102I      550  GCTCAGC              GC^T[ACGT]AGC
              981  GCTCAGC             
BsiHKAI       631  GTGCTC               G[AT]GC[AT]^C
             1122  GTGCTC              
BsmBI        1415  CGTCTC               CGTCTC(1/5)
             1165  GAGACG              
BspMI        1256  ACCTGC               ACCTGC(4/8)
              142  GCAGGT              
BsrBI        1274  CCGCTC               CCGCTC(-3/-3)
             1383  CCGCTC              
BstAPI        624  GCATGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1033  GCAACCTGTGC         
BstYI         360  AGATCC               [GA]^GATC[CT]
              881  GGATCC              
CelII         550  GCTCAGC              GC^T[ACGT]AGC
              981  GCTCAGC             
EarI          316  GAAGAG               CTCTTC(1/4)
             1000  GAAGAG              
FbaI           95  TGATCA               T^GATCA
             1008  TGATCA              
HgaI         1167  GACGC                GACGC(5/10)
              725  GCGTC               
HphI           98  TCACC                GGTGA(8/7)
              728  TCACC               
Ksp632I       316  GAAGAG               CTCTTC(1/4)
             1000  GAAGAG              
MaeIII        727  GTCAC                ^GT[ACGT]AC
             1346  GTCAC               
MflI          360  AGATCC               [GA]^GATC[CT]
              881  GGATCC              
MspA1I        157  CAGCTG               C[AC]G^C[GT]G
             1284  CCGCTG              
NheI           57  GCTAGC               G^CTAGC
              138  GCTAGC              
NspI          322  GCATGC               [GA]CATG^[CT]
             1337  ACATGC              
PaeR7I        335  CTCGAG               C^TCGAG
             1407  CTCGAG              
PflMI         126  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              153  CCAGCAGCTGG         
PleI         1003  GAGTC                GAGTC(4/5)
             1178  GAGTC               
RsaI           52  GTAC                 GT^AC
             1281  GTAC                
SfcI          253  CTGCAG               C^T[GA][CT]AG
             1206  CTGTAG              
SfiI          163  GGCCGCAGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1412  GGCCGTCTCGGCC       
Tsp45I        727  GTCAC                ^GT[GC]AC
             1346  GTCAC               
Van91I        126  CCACAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              153  CCAGCAGCTGG         
XhoI          335  CTCGAG               C^TCGAG
             1407  CTCGAG              
XhoII         360  AGATCC               [GA]^GATC[CT]
              881  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       ItaI        
MaeI        MboI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SmlI        StyI        TaqI        TseI        TspRI       
TthHB8I     
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