Restriction map for pF1KB7744
 (Linear) (Six-base) MAPSORT of: KIBB7744.seq  Check: 2903  from: 1  to: 1461



KIBB7744.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     133    1265    1461 
   Size:        133    1132     196

  Fragments arranged by size: 

                1132     196     133
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     391    1461 
   Size:        262     129    1070

  Fragments arranged by size: 

                1070     262     129
 
AfeI AGC'GCT

Cuts at:      0    1225    1461 
   Size:       1225     236
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     866     898    1461 
   Size:        866      32     563

  Fragments arranged by size: 

                 866     563      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     159     222     312    1461 
   Size:        159      63      90    1149

  Fragments arranged by size: 

                1149     159      90      63
 
ApaI G_GGCC'C

Cuts at:      0     973    1461 
   Size:        973     488
 
AvaI C'yCGr_G

Cuts at:      0     335     523    1229    1407    1461 
   Size:        335     188     706     178      54

  Fragments arranged by size: 

                 706     335     188     178      54
 
AvrII C'CTAG_G

Cuts at:      0     733    1461 
   Size:        733     728
 
BamHI G'GATC_C

Cuts at:      0     881    1461 
   Size:        881     580
 
BanII G_rGCy'C

Cuts at:      0     183     336     664     914     973    1461 
   Size:        183     153     328     250      59     488

  Fragments arranged by size: 

                 488     328     250     183     153      59
 
BbvCI CC'TCA_GC

Cuts at:      0     749    1220    1461 
   Size:        749     471     241

  Fragments arranged by size: 

                 749     471     241
 
BclI T'GATC_A

Cuts at:      0      95    1008    1461 
   Size:         95     913     453

  Fragments arranged by size: 

                 913     453      95
 
BglI GCCn_nnn'nGGC

Cuts at:      0      35     170    1419    1461 
   Size:         35     135    1249      42

  Fragments arranged by size: 

                1249     135      42      35
 
BlpI GC'TnA_GC

Cuts at:      0     551     982    1461 
   Size:        551     431     479

  Fragments arranged by size: 

                 551     479     431
 
Bme1580I G_kGCm'C

Cuts at:      0     973    1019    1461 
   Size:        973      46     442

  Fragments arranged by size: 

                 973     442      46
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     433    1461 
   Size:        433    1028
 
BmtI G_CTAG'C

Cuts at:      0      61     142    1461 
   Size:         61      81    1319

  Fragments arranged by size: 

                1319      81      61
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     417    1433    1461 
   Size:        417    1016      28

  Fragments arranged by size: 

                1016     417      28
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     348     455     869     924     936    1461 
   Size:        281      67     107     414      55      12     525

  Fragments arranged by size: 

                 525     414     281     107      67      55      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     268     749    1220    1461 
   Size:        268     481     471     241

  Fragments arranged by size: 

                 481     471     268     241
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387    1135    1461 
   Size:        387     748     326

  Fragments arranged by size: 

                 748     387     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1112    1461 
   Size:       1112     349
 
BsaAI yAC'GTr

Cuts at:      0     638    1461 
   Size:        638     823
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1273    1303    1461 
   Size:       1273      30     158

  Fragments arranged by size: 

                1273     158      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400     927    1392    1395    1461 
   Size:        400     527     465       3      66

  Fragments arranged by size: 

                 527     465     400      66       3
 
BseYI C'CCAG_C

Cuts at:      0      25     159     215     702    1312    1354    1461 
   Size:         25     134      56     487     610      42     107

  Fragments arranged by size: 

                 610     487     134     107      56      42      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     325    1461 
   Size:        325    1136
 
BsiHKAI G_wGCw'C

Cuts at:      0     635    1126    1461 
   Size:        635     491     335

  Fragments arranged by size: 

                 635     491     335
 
BsmI GAATG_Cn'

Cuts at:      0     818    1461 
   Size:        818     643
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1159    1421    1461 
   Size:       1159     262      40

  Fragments arranged by size: 

                1159     262      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     336     635     664     914     973    1019    1126 
   Size:        183     153     299      29     250      59      46     107

Cuts at:   1126    1461 
   Size:        335

  Fragments arranged by size: 

                 335     299     250     183     153     107      59      46
                  29
 
BspHI T'CATG_A

Cuts at:      0    1047    1461 
   Size:       1047     414
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     133    1265    1461 
   Size:        133    1132     196

  Fragments arranged by size: 

                1132     196     133
 
BsrBI CCG'CTC

Cuts at:      0    1276    1385    1461 
   Size:       1276     109      76

  Fragments arranged by size: 

                1276     109      76
 
BsrFI r'CCGG_y

Cuts at:      0    1303    1461 
   Size:       1303     158
 
BssSI C'ACGA_G

Cuts at:      0     741    1461 
   Size:        741     720
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     630    1039    1461 
   Size:        630     409     422

  Fragments arranged by size: 

                 630     422     409
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     297    1461 
   Size:        297    1164
 
BstYI r'GATC_y

Cuts at:      0     360     881    1461 
   Size:        360     521     580

  Fragments arranged by size: 

                 580     521     360
 
DraIII CAC_nnn'GTG

Cuts at:      0      85    1461 
   Size:         85    1376
 
EaeI y'GGCC_r

Cuts at:      0      73     287     600    1241    1461 
   Size:         73     214     313     641     220

  Fragments arranged by size: 

                 641     313     220     214      73
 
EarI CTCTTCn'nnn_

Cuts at:      0     311     995    1461 
   Size:        311     684     466

  Fragments arranged by size: 

                 684     466     311
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     281     348     391     455     869     924     936 
   Size:        262      19      67      43      64     414      55      12

Cuts at:    936    1461 
   Size:        525

  Fragments arranged by size: 

                 525     414     262      67      64      55      43      19
                  12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280     316     950     964    1407    1461 
   Size:        280      36     634      14     443      54

  Fragments arranged by size: 

                 634     443     280      54      36      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     272     293     429     737     753     969     970    1461 
   Size:        272      21     136     308      16     216       1     491

  Fragments arranged by size: 

                 491     308     272     216     136      21      16       1
            
 
FspI TGC'GCA

Cuts at:      0     679    1461 
   Size:        679     782
 
HaeII r_GCGC'y

Cuts at:      0    1227    1461 
   Size:       1227     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     179     211     636     668    1364    1396    1461 
   Size:        179      32     425      32     696      32      65

  Fragments arranged by size: 

                 696     425     179      65      32      32      32
 
MscI TGG'CCA

Cuts at:      0     602    1461 
   Size:        602     859
 
MspA1I CmG'CkG

Cuts at:      0     159    1286    1461 
   Size:        159    1127     175

  Fragments arranged by size: 

                1127     175     159
 
NaeI GCC'GGC

Cuts at:      0    1305    1461 
   Size:       1305     156
 
NgoMIV G'CCGG_C

Cuts at:      0    1303    1461 
   Size:       1303     158
 
NheI G'CTAG_C

Cuts at:      0      57     138    1461 
   Size:         57      81    1323

  Fragments arranged by size: 

                1323      81      57
 
NspI r_CATG'y

Cuts at:      0     326    1341    1461 
   Size:        326    1015     120

  Fragments arranged by size: 

                1015     326     120
 
PasI CC'CwG_GG

Cuts at:      0     966     988    1461 
   Size:        966      22     473

  Fragments arranged by size: 

                 966     473      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     132     159    1461 
   Size:        132      27    1302

  Fragments arranged by size: 

                1302     132      27
 
PmlI CAC'GTG

Cuts at:      0     638    1461 
   Size:        638     823
 
PpuMI rG'GwC_Cy

Cuts at:      0     429    1461 
   Size:        429    1032
 
PspOMI G'GGCC_C

Cuts at:      0     969    1461 
   Size:        969     492
 
PspXI vC'TCGA_Gb

Cuts at:      0     335    1407    1461 
   Size:        335    1072      54

  Fragments arranged by size: 

                1072     335      54
 
PstI C_TGCA'G

Cuts at:      0     257    1461 
   Size:        257    1204
 
PvuII CAG'CTG

Cuts at:      0     159    1461 
   Size:        159    1302
 
SanDI GG'GwC_CC

Cuts at:      0     429    1461 
   Size:        429    1032
 
SexAI A'CCwGG_T

Cuts at:      0     565    1461 
   Size:        565     896
 
SfcI C'TryA_G

Cuts at:      0     253    1206    1461 
   Size:        253     953     255

  Fragments arranged by size: 

                 953     255     253
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     170    1419    1461 
   Size:        170    1249      42

  Fragments arranged by size: 

                1249     170      42
 
SmaI CCC'GGG

Cuts at:      0    1231    1461 
   Size:       1231     230
 
SmlI C'TyrA_G

Cuts at:      0     335     402    1150    1407    1461 
   Size:        335      67     748     257      54

  Fragments arranged by size: 

                 748     335     257      67      54
 
SphI G_CATG'C

Cuts at:      0     326    1461 
   Size:        326    1135
 
StuI AGG'CCT

Cuts at:      0     368    1461 
   Size:        368    1093
 
StyI C'CwwG_G

Cuts at:      0     296     733     757    1461 
   Size:        296     437      24     704

  Fragments arranged by size: 

                 704     437     296      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1094    1461 
   Size:       1094     367
 
Tth111I GACn'n_nGTC

Cuts at:      0     567    1461 
   Size:        567     894
 
XbaI T'CTAG_A

Cuts at:      0     463    1461 
   Size:        463     998
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     595    1461 
   Size:        595     866
 
XhoI C'TCGA_G

Cuts at:      0     335    1407    1461 
   Size:        335    1072      54

  Fragments arranged by size: 

                1072     335      54
 
XmaI C'CCGG_G

Cuts at:      0    1229    1461 
   Size:       1229     232
 
XmnI GAAnn'nnTTC

Cuts at:      0    1198    1461 
   Size:       1198     263
 

 Enzymes that do cut:

     AarI     AcuI     AfeI     AloI    AlwNI     ApaI     AvaI    AvrII
    BamHI    BanII    BbvCI     BclI     BglI     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI    BstYI   DraIII
     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I
     MscI   MspA1I     NaeI   NgoMIV     NheI     NspI     PasI    PflMI
     PmlI    PpuMI   PspOMI    PspXI     PstI    PvuII    SanDI    SexAI
     SfcI     SfiI     SmaI     SmlI     SphI     StuI     StyI    TaqII
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
     BanI     BbeI     BbsI     BcgI    BciVI    BfrBI    BglII    BmgBI
    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI    BspEI    BsrDI    BsrGI
   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI
     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI     PpiI
    PshAI     PsiI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SrfI     SspI     SwaI     TatI     TstI     ZraI
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