Restriction map for pF1KB7740
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstXI       BstYI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraIII      DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HaeIII      HapII       Hin1I       HpaII       KasI        
KpnI        KspI        MaeI        MamI        MflI        
MluI        MluNI       MroI        MscI        MspI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspI        StuI        StyI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AccI          575  GTCGAC               GT^[AC][GT]AC
AlwI          389  GGATC                GGATC(4/5)
AlwNI         145  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          826  CCTCAGG              CC^T[ACGT]AGG
BciVI         436  GGATAC               GTATCC(6/5)
BpmI         1205  CTCCAG               CTGGAG(16/14)
BseRI        1168  GAGGAG               GAGGAG(10/8)
BspMI         130  GCAGGT               ACCTGC(4/8)
BsrDI         179  GCAATG               GCAATG(2/0)
BstBI        1085  TTCGAA               TT^CGAA
Bsu36I        826  CCTCAGG              CC^T[ACGT]AGG
CfoI          374  GCGC                 GCG^C
DraI          849  TTTAAA               TTT^AAA
DraII         132  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DrdI          732  GACAACAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        826  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      132  AGGTCCT              [GA]G^G[ACGT]CC[CT]
HgaI          376  GCGTC                GACGC(5/10)
HhaI          374  GCGC                 GCG^C
HinP1I        374  GCGC                 G^CGC
HindIII      1246  AAGCTT               A^AGCTT
HpaI          621  GTTAAC               GTT^AAC
MaeII        1439  ACGT                 A^CGT
MfeI         1190  CAATTG               C^AATTG
MunI         1190  CAATTG               C^AATTG
NspV         1085  TTCGAA               TT^CGAA
PpuMI         132  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI           51  CTGCAG               CTGCA^G
SalI          575  GTCGAC               G^TCGAC
SfcI           51  CTGCAG               C^T[GA][CT]AG
SfuI         1085  TTCGAA               TT^CGAA
SmlI         1163  CTCAAG               C^T[CT][GA]AG
SphI          672  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         102  CGCG                 CG^CG
              375  CGCG                
Asp700I       874  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1078  GAAAGAATTC          
AvaII         133  GGTCC                G^G[AT]CC
              963  GGACC               
BbsI          694  GAAGAC               GAAGAC(2/6)
              970  GAAGAC              
BpuAI         694  GAAGAC               GAAGAC(2/6)
              970  GAAGAC              
BsgI          866  GTGCAG               GTGCAG(16/14)
             1441  GTGCAG              
BsmFI         141  GGGAC                GGGAC(10/14)
             1436  GGGAC               
BsmI          613  GAATGC               GAATGC(1/-1)
             1059  GCATTC              
BstUI         102  CGCG                 CG^CG
              375  CGCG                
Cfr13I        133  GGTCC                G^G[ACGT]CC
              963  GGACC               
EarI          782  CTCTTC               CTCTTC(1/4)
              262  GAAGAG              
FauI          100  CCCGC                CCCGC(4/6)
              365  CCCGC               
HincII        575  GTCGAC               GT[CT]^[GA]AC
              621  GTTAAC              
HindII        575  GTCGAC               GT[CT]^[GA]AC
              621  GTTAAC              
Ksp632I       782  CTCTTC               CTCTTC(1/4)
              262  GAAGAG              
MslI           78  CATGGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              442  CACTATAATG          
MvnI          102  CGCG                 CG^CG
              375  CGCG                
MwoI         1000  GCGGTTGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1248  GCTTCAGGAGC         
NlaIV         159  GGAACC               GG[ACGT]^[ACGT]CC
             1254  GGAGCC              
NspI          551  ACATGC               [GA]CATG^[CT]
              672  GCATGC              
Sau96I        133  GGTCC                G^G[ACGT]CC
              963  GGACC               
TfiI          802  GATTC                G^A[AT]TC
             1290  GATTC               
Tsp45I        710  GTGAC                ^GT[GC]AC
              724  GTGAC               
XmnI          874  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1078  GAAAGAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsaJI       BsiYI       BslI        BsmAI       
Bsp1407I    BsrGI       BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       EcoRI       EcoRII      
Fnu4HI      FokI        HinfI       HphI        ItaI        
MaeIII      MboI        MboII       MnlI        MseI        
MspA1I      MvaI        NdeII       NlaIII      PleI        
PvuII       RsaI        Sau3AI      ScrFI       SfaNI       
SspBI       TaqI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     
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